Application Notes

Are you looking for a unique, low-volume syringe that holds metal tips (with needles as small as 36 gauge) as well as glass or quartz tubing? NanoFil is the answer. Necessity is the mother of invention” This quote from the dialogue Republic by ancient Greek philosopher Plato, could be considered one of the mantras of scientific research and product development. Many specialized products used in research were developed out of a specific need that was not already addressed in the market place. We often use these products without even thinking about their history, their development, or the imagination of the person who invented them. WPI's NanoFil

Parts List Qty Part Number Description 2 13163 MALE LUER FITTING 5/32 2 13161 MALE LUER FITTING FOR 3/32 2 13157

A low-noise amplifier like the DAM50 is an excellent choice for EEG recording in rodents. WPI’s amplifiers were engineered for the bio-medical researcher. While 20-30μV of noise is common in bio-amplifiers, WPI’s DAM series amplifiers generate 0.4μV RMS (root mean squared) at 0.1-100Hz. (That’s equal to about 2μV peak to peak.) This setup shows one way such recordings could be made. The RC1 electrode works well for rats, and the EP1 is more suitable for mouse cranial application. For this application, you will need the following equipment: (1) DAM50 amplifier (1) MD4R micromanipulator (2) M

Watch how researchers from the University of Chicago inject adult zebrafish using a 10μl NanoFil microsyringe controlled by a Micro4 controller and UltraMicroPump III (UMP3-1 includes one UMP3 pump and a Micro4 controller).  More Info References Warmerdam, T., Schröder, F., Wit, H., & Albers, F. (n.d.). Perilymphatic and endolymphatic pressures

In this JoVE video you can observe as the researchers perform an intraluminal middle cerebral artery occlusion (MCAO) on a mouse. The MCAO model is the most frequent used model among experimental ischemic stroke models. WPI instruments used in this application include: SuperCut Iris scissor, straight 14218 Vannas micro-scissor, straight 14003 Dumont fine tip forceps 503290 Vessel clip

Chiara Cianciolo Cosentino, at the University of Pittsburgh, describes how she uses intravenous microinjections of zebrafish larvae to study acute kidney injury in this JoVE video. You can also watch this video on JoVE. WPI equipment shown in this video includes: MMP Manual Microsyringe Pump PV820 Pneumatic PicoPump M3301 Manual Micromanipulator M10 Magnetic Stand 5052 Steel Base Plate

Muscle contraction and relaxation is caused by the attachment and detachment of two types of molecules (actin and myosin) to each other within the fibers that compose a muscle. These molecules are arranged as overlapping filaments within the functional units of the muscle fibers that are known as sarcomeres. Each crossbridge that is made between the end of a myosin molecule and a binding site on an actin molecule requires a molecule of ATP, an energy source, to be hydrolyzed when the end of the myosin molecule is released from the actin filament. After the release, the myosin molecule is ready to move down to another actin binding site causing the sarcomere, and the muscle, to shorten. When ATP is regenerated through a series of enzymatic reactions, another molecule, NADH, provides the energy needed for the regeneration of the ATP. The NADH fluoresces when exposed to ultraviolet (UV) light. The oxidized form of the molecule NAD+, which has lost its stored energy, does not fluoresce.

The new SI-BF-100 is an LED-based fluorometer for life science applications. With up to seven LED modules (wavelengths), the SI-BF-100 covers many fluorometric applications in both Neuroscience and Cell Biology. This technology significantly cuts the cost of fluorescent imaging without sacrificing resolution or quality. WPI's recent introduction of the high intensity LED version of the Biofluorometer opens a host of new applications for life science researchers.  In Vivo Applications Monitor electrical activity over large areas of intact brain without the limitations of microelectrode arrays Simultaneously monitor electrical activity an

WPI surgical instruments were recently featured in a JoVE video that demonstrates a new method for cross pollinating grasses.   More Info Jiang, H., Barbier, H., Brutnell, T. Methods for Performing Crosses in Setaria viridis, a New Model System for the Grasses. J. Vis. Exp. (80), e50527, doi:10.3791/50527 (2013).

Researchers at the University of Michigan are using WPI's PV820 for injecting a morpholino solution into the lumen of the otic vesicle of 1-day old zebrafish embryos.  Then, they use electroporation to introduce mif and mif-like morpholinos into the developing inner ear tissues. Check out the JOVE video