Application Notes

Are you looking for a microliter or sub-microliter and high precision syringe that holds needles as small as 36 gauge (G), in addition to having the capability to connect to quartz tubing? WPI’s NanoFil is the answer. We offer NanoFil syringes with NanoFil needles or the option to connect the NanoFil syringe to quartz tubing to use in research studies, mainly involving sub-microliter volume injections into animal tissues. Why Select WPI’s NanoFil (Syringe and Needle) System? Low Dead Volume The dead volume of NanoFil's is minute or neglig

Parts List Qty Order code Description 2 13163 MALE LUER FITTING 5/32 2 13161 MALE LUER FITTING FOR 3/32 2 13157

A low-noise amplifier like the DAM50 is an excellent choice for EEG recording in rodents. WPI's amplifiers were engineered for the bio-medical researcher. While 20-30μV of noise is common in bio-amplifiers, WPI’s DAM series amplifiers generate 0.4μV RMS (root mean squared) at 0.1-100Hz. (That’s equal to about 2μV peak to peak.) This setup shows one way such recordings could be made. The RC1 electrode works well for rats, and the EP1 is more suitable for mouse cranial application. For this application, you will need the following equipment: (1) DAM50 amplifier (1) MD4R micromanipulator (2) M3301EH electrode holders (2) Profile A single tungsten metal electrodes (1) M10 stand (1) 5052 steel base plate (1) 5470 cable (1) electrode (RC1 or EP1) Rat Cranial Applications The RC1 electrode comes with a 4' unterminated wire attached. That needs to be soldered to the green-white ground wire of the 5470 cable. The red wire from the 5

Watch how researchers from the University of Chicago inject adult zebrafish using a 10μl NanoFil microsyringe controlled by a Micro4 controller and UltraMicroPump III (UMP3-1 includes one UMP3 pump and a Micro4 controller).  More Info References Warmerdam,

In this JoVE video you can observe as the researchers perform an intraluminal middle cerebral artery occlusion (MCAO) on a mouse. The MCAO model is the most frequent used model among experimental ischemic stroke models. WPI instruments used in this application include: SuperCut Iris scissor, straight 14218 Vannas micro-scissor, straight 14003 Dumont fine tip forceps 503290 Vessel clip 14120 High Frequency Desiccator 900 504043 Silicone RTV adhesive 1571 Thermal Cautery Unit 501292 BAT-12 BAT-12R Ansari, S., Azari, H., McConnell, D. J., Afzal, A., Mocco, J. Intraluminal Middle Cerebral Artery Occlusion (MCAO) Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Mice. J. Vis. Exp. (51), e2879, doi:10.3791/2879 (2011).

Chiara Cianciolo Cosentino, at the University of Pittsburgh, describes how she uses intravenous microinjections of zebrafish larvae to study acute kidney injury in this JoVE video. You can also watch this video on JoVE. WPI equipment shown in this video includes: MMP Manual Microsyringe Pump PV820 Pneumatic PicoPump M3301 Manual Micromanipulator M10 Magnetic Stand 5052 Steel Base Plate

WPI surgical instruments were recently featured in a JoVE video that demonstrates a new method for cross pollinating grasses.   More Info Jiang, H., Barbier, H., Brutnell, T. Methods for Performing Crosses in Setaria viridis, a New Model System for the Grasses. J. Vis. Exp. (80), e50527, doi:10.3791/50527 (2013).

Researchers at the University of Michigan are using WPI's PV820 for injecting a morpholino solution into the lumen of the otic vesicle of 1-day old zebrafish embryos.  Then, they use electroporation to introduce mif and mif-like morpholinos into the developing inner ear tissues. Check out the JOVE video

In this video, Mike Pizza demonstrates how to front fill a Nanofil syringe using MicroFil.   More Info

Abstract Concentrations of DNA in solution (31µg/mL and 561µg/mL) were measured with a spectrometer and UV/Vis light source in a cuvette. A 2mm pathlength cuvette does not require a pre-measurement dilution within this concentration range, thus a potential source of error was eliminated. Although a 2mm cuvette has a total internal volume of 0.7mL, only 350µL is required to obtain an accurate measurement. Experimental Procedure Standard solutions of DNA (Sigma D1626) were prepared gravimetrically using 18.2MΩ/cm ultrapurified water as a solvent. Solutions were prepared between 0.0µg/mL and 561.1µg/mL. Measurements were taken in triplicate using a 2mm cuvette (WPI #CUV2102-1) in a standard cuvet