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Microinjection

  1. Affordable New Injector: Configure the PV850 Software
    September 08, 2020
    [by Gabe Gonzalez] The PV850 Injector is designed to simplify intracellular injection and a variety of other microinjection tasks. The PV850 uses regulated air pressure for injecting cells with fluid. Injected volumes range from picoliters to nanoliters. The port supplies positive pressure for high-pressure ejection maximum of 8 7PSI. The PV850 Microinjector offers separate regulated compensation (back filling prevention) and ejection pressures with a precision timing circuit that switches from injection pressure to compensation pressure automatically. Timing, injection pressure and compensation pressure are adjusted independently using the intuitive touch-screen user interface. Time intervals can range from 2 seconds down to
  2. Affordable New Injector: Setup the PV850 System
    September 08, 2020
    [by Gabe Gonzalez] The PV850 Injector is designed to simplify intracellular injection and a variety of other microinjection tasks. The PV850 uses regulated air pressure for injecting cells with fluid. Injected volumes range from picoliters to nanoliters. The port supplies positive pressure for high-pressure ejection maximum of 87 PSI. The PV850 Microinjector offers separate regulated compensation (back filling prevention) and ejection pressures with a precision timing circuit that switches from injection pressure to compensation pressure automatically. Timing, injection pressure and compensation pressure are adjusted independently using the intuitive touch-screen user interface. Time intervals can range from 2 seconds down to
  3. Setting up the WPI PicoPump
    September 03, 2020
    Designed to simplify intracellular injection and a variety of other micro­in­jec­tion tasks, WPI’s PicoPumps (PV830 and PV820) use precisely regulated pres­sures for se­cur­ing cells and injecting them with fluid. Injected volumes range from picoliters to nanoliters. Separate ports supply positive and negative pressure—pos­i­tive pressure for high-pressure ejection, and suction for supporting the cell or for filling the pipette from the tip. In this quick series, you can see how to setup a PicoPump. 1. What's Included with a PV Pump In this first video (#1) you can see all the components of the system as they unpack the system.  
  4. Popular UMP3 Injector is Not Your Ordinary Syringe Pump
    July 13, 2020
    [by Alec Dickson] Easy to Configure Using the SmartTouch Interface The UMP3 UltraMicroPump is easy to configure using the SMARTouch interface. It is pre-programmed for popular microsyringes and allows for user-defined syringe parameters, too. This ensures nearly universal compatibility. You can control up to two pumps, either simultaneously or independently, and the optional foot switch means hands-free operation. You can instantly verify actual injection volume on the graphical display. You can even use the touch screen while wearing gloves. Ensure Reliable Injections of Small Volumes The extremely fine thread-pitch of the UMP3 drive shaft ensures reliable injections
  5. Protect Cell Survival and Improve Research Results with Fluorodishes Cell Culture Dishes
    June 16, 2020
    [by Alec Dickson]  WPI's FluoroDish™ tissue culture dishes provide exceptional imaging quality for many applications requiring the use of inverted microscopes such as high-resolution image analysis, microinjection and electrophysical recording of fluorescent-tagged cells. We have a 50 mm diameter dish and two types of 35 mm diameter dishes.        Better O
  6. CardioPhys ECG is Perfect for Affordable Cardiotoxicity Testing in Small Animals
    May 18, 2020
    [by Mikaël Berrou] CardioPhys™ and Cardiotoxicity When a new drug is discovered with an effect against a pathology, a natural public demand would ask to administrate it immediately. Although the new drug may be beneficial in many aspects, that new molecule could potentially have side effects that need to be evaluated. The most important potential side effect is cardiotoxicity. What is Cardiotoxicity? Cardiotoxicity is the damage to the heart by harmful chemicals (www.nccn.org/patients/resources/life_with_cancer/managing_symptoms/cardiac_toxicity.aspx), and its evaluation ha
  7. Versatile Microinjection Syringe Pump (UMP3T) for Injections in the Nanoliter to Milliliter Range
    May 15, 2020
    The UltraMicroPump3 is a versatile microinjection syringe pump designed to support the needs of a busy lab. The UMP3 Microinjector is suitable for delivery of media from nanoliters to milliliters. the UMP3 injecotor’s user interface and system design enables delivery of highly accurate and repeatable injections. The choice for thousands of scientists, it is widely cited in research papers.   Intuitive Touchscreen Display for Easy Setup The SMARTouch controller has an intuitive touch screen interface and an easy-to-use menu for quick configuration of the syringe size, volumes and f
  8. 8 Good Reasons to Choose the MICRO-ePUMP Microinjector for your Applications
    April 10, 2020
    Do you need a precision microinjector with an integrated pressure source for injections in the picoliter range? WPI’s new MICRO-ePUMP, which is comparable to the Ependorf Femtojet, is a portable solution for injecting DNA, RNA and proteins, as well as pre- and post- implantation in embryos. Whether you are doing disease modeling with zebrafish or viral research for SARS-CoV2, the MICRO-ePUMP has much to offer. Here are eight good reasons to choose the MICRO-ePUMP for your applications.   Convenient internal pressure source The convenient internal pressure source i
  9. Genetic Transfer (viral or non-viral vector) Using Microprocessor-Controlled Injector
    March 02, 2020
    With the first approved human gene therapy trial in 19891 (Rosenberg, et.al.), gene therapy has come a long way in modern medicine and is making inroads in clinics and the market in general.2,3 2017 was an important year for gene therapy when Luxurna, the first human gene therapy drug for an inherited retinal dystrophy, was approved by Food and Drug Administration (USA).4 Now several drugs are undergoing clinical trials. With an estimated $11 billion (USD) market in the next 10 years, both clinical trials and the pharmaceutical industry are expected to benefit immensely from gene therapy.5 With improved use of viral vectors (Adeno-associated virus (AAV), adenovirus, lentivirus, retrovirus, HSV) or non-viral vectors, research and clinical trials on the development of therapeutic genes have witnessed great
  10. New Technology Makes Microinjection More Reliable, Repeatable and Affordable
    January 24, 2020
    Microinjection is the process of transferring genetic materials into a living cell using glass micropipettes or metal microinjection needles. Glass micropipettes can be of various sizes with tip diameters ranging from 0.1 to 10 µm. DNA or RNA is injected directly into the cell’s nucleus. Microinjection has been successfully used with large frog eggs, mammalian cells, mammalian embryos, plants and tissues. Microinjection has been expensive, can be a slow process and requires skilled personnel, but new technologies are making it even more reliable, repeatable and affordable. Pronuclear injection, inserting DNA or RNA into the nucleus of a fertilized oocyte to create transgenic organisms, lets researchers study the role of particular genes. This horizontal gene tr
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