1. All Topics
  2. Videos
  3. 900A Micropressure System
  4. Testimonial
  5. Product Information

Microinjection

  1. Using the 900A Micropressure System for Measuring Pressure in Small Blood Vessels and Cells
    July 16, 2018
    This 900A video series of 6 videos is designed to help familiarize you with the basic operation of the 900A Micropressure System. The 900A was designed for measuring pressures in kidney tubules, and it has many applications. It has recently been used for measuring ventricular blood pressure in larval fish. We suggest that you become familiar with the information in the instruction manual and the parts and techniques you need to operate the system effectively. Please read the manual and watch these videos prior to setting up and testing your 900A Micropressure System.  Instrument Description The 900A Micropressure System is designed to measure pressures from –200 to +400 mmHg in sma
  2. Replacing the Gasket in a Nanoliter2010
    February 13, 2018
    In this video you'll see how to replace the gasket in a Nanoliter2010.    More Info If you have questions, contact us at 866.606.1974 (US Toll Free) or at wpi@wpiinc.com. To request a formal quote, please email us at quotes@wpiinc.com.
  3. NanoFil for Sub-Microliter Injection in Small Animal Research
    June 25, 2015
    Are you looking for a unique, low-volume syringe that holds metal tips (with needles as small as 36 gauge) as well as glass or quartz tubing? NanoFil is the answer. Necessity is the mother of invention” This quote from the dialogue Republic by ancient Greek philosopher Plato, could be considered one of the mantras of scientific research and product development. Many specialized products used in research were developed out of a specific need that was not already addressed in the market place. We often use these products without even thinking about their history, their development, or the imagination of the person who invented them. WPI's NanoFil
  4. Application Kits for Use with the NanoFil for Microinjection
    June 25, 2015
      Application kits are specially designed for eye research for injecting retinal pigment epithelium (RPE) and for intraocular (IO) injections. In addition, these kits may be used for brain injection in mice. They need to be used with a NanoFil syringe and UMPIII to achieve accurate, repetitive and oil-free injection in the submicroliter range. Each kit includes two pieces of Silflex tubing (one for a spare), a holder assembly, spare gaskets, and an assortment of four tips - blunt for the RPE kit and beveled tips for the IO kit. Each kit comes with one each of 33, 34, 35 and 36 gauge tips so that first time users can find the best size for their application.The Silflex tubing is the most critical component of the kit. This 35cm long, flexible tubing has a very precise outer
  5. When a Bigger Bolus is Better
    June 12, 2015
    First, let's consider volumes. A milliliter (mL) is one thousandth the volume of a liter (L) or 10-3L A microliter (µL) is one thousandth the volume of a mL (10-6L) A nanoliter (nL) is one thousandth the volume of a µL (10-9L) A picoliter (pL) is one thousandth the volume of a nL (10-12L) This is graphically represented at the right. Notice that the mL is one trillion times larger than the picoliter. The table (right) shows that the side of a cube with a volume of 1mL is 1cm long. Likewise, the side of a cube with a volume of 1pL is 10µm long. Just for comparison, it shows that the diameter of a sphere with a volume of 1mL is 1.24cm, and the volume of a sphere with a volume of 1pL is 12.4µm. With these volume comparisons in
  6. Using WPI's PUL-1000 Micropipette Puller
    March 06, 2015
    World Precision Instruments' PUL-1000 is a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes used in intracellular recording, microperfusion and microinjection. It offers programmable sequences of up to four steps with complete control over the heating, force, movement and cooling time. This allows graduated cycles for a variety of applications. PUL-1000 can produce pipettes with tip diameters from less than 0.1µm to 10+ µm.  More Info
  7. Nanoliter 2010, Perfect for Microinjection
    July 21, 2014
    WPI's Nanoliter 2010 Microinjection Pump is ideal for many applications, including zebrafish, xenopus oocytes and drosophila. MICRO4, an optional microprocessor-based controller, can provide an "intelligent" and easy-to-use interface to up to four Nanoliter Injectors. Operating parameters are set with the membrane key-pad and LCD display. More Info
  8. Microinjection Tool Box
    July 10, 2014
    The system depicted includes components often favored by researchers: PV820 Pneumatic PicoPump, PUL-1000 Micropipette Puller, M4C stand,
  9. Zebrafish Microinjection using the UMP3/Micro4
    July 08, 2014
    Watch how researchers from the University of Chicago inject adult zebrafish using a 10μl NanoFil microsyringe controlled by a Micro4 controller and UltraMicroPump III (UMP3-1 includes one UMP3 pump and a Micro4 controller).  More Info References Warmerdam, T., Schröder, F., Wit, H., & Albers, F. (n.d.). Perilymphatic and endolymphatic pressures
  10. Zebrafish Microinjection Technique from JoVE
    June 25, 2014
    Chiara Cianciolo Cosentino, at the University of Pittsburgh, describes how she uses intravenous microinjections of zebrafish larvae to study acute kidney injury in this JoVE video. You can also watch this video on JoVE. WPI equipment shown in this video includes: MMP Manual Microsyringe Pump PV820 Pneumatic PicoPump M3301 Manual Micromanipulator M10 Magnetic Stand 5052 Steel Base Plate
Page
Copyright © World Precision Instruments. All rights reserved.