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Microinjection

  1. Get Familiar with Your New PUL-1000 Micropipette Puller
    April 09, 2021
    Let’s look at the PUL-1000, a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes. Here we will examine the design of the unit.         Tempered Glass Lid For safety, the lid should be closed whenever you are pulling glass or moving the unit. Capillary Glass Clamp Pads These two silicone clamp pads securely hold the glass when you are pulling pipettes. Check the glass OD and install appropriate pads accordingly. Heating Filament The PUL-1000 comes with a circular platinum/iridium box filament, which may be easily replaced w
  2. Pre-Pulled Micropipettes, Perfect for Closed Container Integrity Testing
    February 02, 2021
      WPI pre-pulled pipettes (MicroTips) are a popular choice for pharmaceutical companies carrying out CCIT (Closed Container Integrity Testing). Each MicroTip is individually tested and inspected and can be shipped with a certificate of compliance. CCIT is an important part in understanding the biological safety and suitability of primary packaging. It is used to assess the potential of the container closure system to maintain a sterile barrier or to prevent leakage resulting in contamination or loss of the drug. A good overview of Understanding Container
  3. Microinjection Tool Box
    January 29, 2021
    The system depicted includes components often favored by researchers: MICRO-ePUMP Pneumatic PicoPump with built in MICRO-ePORE™ Cell Penetrator to facilitate microinjections SU-P1000 Micropipette Puller M4C Stand M3301R Micromanipulator PZMTIII Microscope with Optional Lighted Base with Articulating Mirror and optional PRO-300 HDS Camera and View Screen E2XX  Micropipette Storage Jar Z-MOLDS Microinjection and Transplantation Molds 14003-G Vannas spring scissors Glass Capillaries 77020 Glass Tweezers FluoroDish Optical Grade Glass Bottom Culture Dishes Whatever your needs, WPI offers a range of equipment to fill your requirements. Download Microinjection Systems Brochure   Empowering Scientists with Reliable Instruments Serving scientists for over 50 years, WPI offers a variety of instruments for microinjection including pumps, pipetters, microscopes and more. One of our most popular pumps for microinjection is the PV850 Pneumatic PicoPump. The PV850, PV830, uPump and MICRO-ePUMP were designed to simplify intracellular injection. You get repeatable microinjection in volumes ranging from picoliters to nanoliters. The volume injected is controlled by the inside diameter of the glass tip, the pressure and the time. The PV850 offers eject and hold pressure. The hold pressure prevents backfilling of the pipette by capillary action, ensuring repeatable injections. In addition, the PV830 also has vacuum pressure which allows you to securely hold a cell with one pipette while you inject it with another.  The uPUMP and MICRO-ePUMP have a built in pressure source, eliminating the need for an external pressure supply. The MICRO-ePUMP with Integrated Cell Penetrator and Internal Pressure Source is perfect for intracellular injection. The Cell Penetrator delivers a highly localized voltage signal to a targeted injection site to facilitate penetration with minimal trauma. WPI h
  4. Instruments for Zebrafish Research - Oocytes, Embryos, Larvae and Adults
    January 28, 2021
    World Precision Instruments (WPI) offers a broad range of research instruments for zebrafish (Danio Rerio) research, from the time the genetics of the subject are modified through the point where the physiological effects of the change are monitored. Whether researchers are performing microinjection or making cardiovascular measurements, the WPI product line includes instruments for research on oocytes, embryos, larvae and adult zebrafish. Enhanced Microinjection Efficiency for Genetic Modification   The customizable Zebrafish Microinjection Toolbox
  5. Affordable New Injector: Configure the PV850 Software
    September 08, 2020
    [by Gabe Gonzalez] The PV850 Injector is designed to simplify intracellular injection and a variety of other microinjection tasks. The PV850 uses regulated air pressure for injecting cells with fluid. Injected volumes range from picoliters to nanoliters. The port supplies positive pressure for high-pressure ejection maximum of 8 7PSI. The PV850 Microinjector offers separate regulated compensation (back filling prevention) and ejection pressures with a precision timing circuit that switches from injection pressure to compensation pressure automatically. Timing, injection pressure and compensation pressure are adjusted independently using the intuitive touch-screen user interface. Time intervals can range from 2 seconds down to
  6. Affordable New Injector: Setup the PV850 System
    September 08, 2020
    [by Gabe Gonzalez] The PV850 Injector is designed to simplify intracellular injection and a variety of other microinjection tasks. The PV850 uses regulated air pressure for injecting cells with fluid. Injected volumes range from picoliters to nanoliters. The port supplies positive pressure for high-pressure ejection maximum of 87 PSI. The PV850 Microinjector offers separate regulated compensation (back filling prevention) and ejection pressures with a precision timing circuit that switches from injection pressure to compensation pressure automatically. Timing, injection pressure and compensation pressure are adjusted independently using the intuitive touch-screen user interface. Time intervals can range from 2 seconds down to
  7. Setting up the WPI PicoPump
    September 03, 2020
    Designed to simplify intracellular injection and a variety of other micro­in­jec­tion tasks, WPI’s PicoPumps (PV830 and PV820) use precisely regulated pres­sures for se­cur­ing cells and injecting them with fluid. Injected volumes range from picoliters to nanoliters. Separate ports supply positive and negative pressure—pos­i­tive pressure for high-pressure ejection, and suction for supporting the cell or for filling the pipette from the tip. In this quick series, you can see how to setup a PicoPump. 1. What's Included with a PV Pump In this first video (#1) you can see all the components of the system as they unpack the system.  
  8. Popular UMP3 Injector is Not Your Ordinary Syringe Pump
    July 13, 2020
    [by Alec Dickson] Easy to Configure Using the SmartTouch Interface The UMP3 UltraMicroPump is easy to configure using the SMARTouch interface. It is pre-programmed for popular microsyringes and allows for user-defined syringe parameters, too. This ensures nearly universal compatibility. You can control up to two pumps, either simultaneously or independently, and the optional foot switch means hands-free operation. You can instantly verify actual injection volume on the graphical display. You can even use the touch screen while wearing gloves. Ensure Reliable Injections of Small Volumes The extremely fine thread-pitch of the UMP3 drive shaft ensures reliable injections
  9. Protect Cell Survival and Improve Research Results with Fluorodishes Cell Culture Dishes
    June 16, 2020
    [by Alec Dickson]  WPI's FluoroDish™ tissue culture dishes provide exceptional imaging quality for many applications requiring the use of inverted microscopes such as high-resolution image analysis, microinjection and electrophysical recording of fluorescent-tagged cells. We have a 50 mm diameter dish and two types of 35 mm diameter dishes.        Better O
  10. CardioPhys ECG is Perfect for Affordable Cardiotoxicity Testing in Small Animals
    May 18, 2020
    [by Mikaël Berrou] CardioPhys™ and Cardiotoxicity When a new drug is discovered with an effect against a pathology, a natural public demand would ask to administrate it immediately. Although the new drug may be beneficial in many aspects, that new molecule could potentially have side effects that need to be evaluated. The most important potential side effect is cardiotoxicity. What is Cardiotoxicity? Cardiotoxicity is the damage to the heart by harmful chemicals (www.nccn.org/patients/resources/life_with_cancer/managing_symptoms/cardiac_toxicity.aspx), and its evaluation ha
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