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- Microinjection processes use either metal microinjection needles or glass micropipettes to inject small liquid volumes. For example, genetic material may be inserted into a living cell, a drug introduced into an eye or brain, or fluid injected into a muscle. Typically, microinjection is performed under a microscope. A stereotaxic frame setup may be required.
Recently, WPI introduced its customizable Microinjection System with everything you need to get started. We can help you customize your system with many options and microinjection system accessories. The basic system includes:
Microinjection pump like the PV850 Microinjector with External Pressure Source
LED lighted microscope base with a PZMIII Stereo Microscope and an articulating mirror
WPI offers a variety of pumps along with special syringes, stereotaxic frames, glass capillaries and needles. The setup you choose depends on the size of your microinjection aliquots, the volume to be injected and the size of needle or glass tip you choose. In addition, we offer an electroporator for transfection procedures.
Whether you are working with Danio rerio (zebrafish), Xenopus, Drosophila or Caenorhabditis elegans, we can help you customize a microinjection system for your application.
- During the last 30 years, TEER measurements have become universally established as the most convenient, reliable and non-destructive method of evaluating and monitoring the growth of epithelial tissue culture in vitro. The confluence of the cellular monolayer is quickly determined by a sharp increase in TEER. TEER measurement technology, first introduced to tissue and cell biologist by WPI in the mid '80s has since been perfected and expanded to include a range of TEER related manual and automatic instrumentation.
For epithelial physiology studies, our Ussing systems offer a quick, effective means of making low-resistance electrical connections to the Ussing chamber without the need of long agar bridges. Ag/AgCl half-cells screw into short tubes which plug firmly into place in the chamber's luer ports, eliminating the inconvenience and expense of Calomel half-cells in open liquids.
- Better Imaging Results with Optical-Grade Glass Bottom Dishes
WPI's optical quality glass bottom FluoroDish Cell Culture dishes offer better imaging quality. There are several varieties that let life science researchers work with small sample volumes. This is necessary when researchers are working with expensive chemicals or experimental drugs. They are designed with the lowest access angle for easier insertion of a micropipette during cellular microinjection. They are perfect for live cell imaging and embryo research.
Cited in hundreds of reference articles, our optical grade, glass bottom dishes are unique in the marketplace and conform to strict quality control standards.
- Coating surgical instruments with a black ceramic adds a thin layer to the metal instrument, making the instrument harder and giving you greater precision. This anti-glare surface minimizes reflections off the surface of your instruments.
- Surgical tweezer, forceps, needle pullers – here's some information on selecting the proper forceps for your application.
Choosing Surgical Forceps
Surgical forceps may be broadly divided into two categories, thumb forceps (frequently called surgical tweezers or pinning forceps) and ring forceps (also called hemostats, hemostatic forceps and locking forceps).
Thumb forceps are spring forceps used by compression between your thumb and forefinger and are used for grasping, holding or manipulating body tissue. They are non-ratchet style. For example, you could use thumb forceps to hold or move tissue during surgery or to move dressings.
Hemostatic forceps are hinged forceps that look more like scissors. Hinged forceps may come with or without a "lock" for clamping.
Thumb forceps are available with a variety of tips. The tips may be flat, serrated, cupped, ringed, grooved, diamond dusted or have teeth. The tips may also be straight, curved or angled. See the images below. Serrated tweezers (thumb forceps) are designed for use with tissues. The serrations or teeth actually cause less damage than flat forceps, because it requires less pressure to maintain a firm grip. Use smooth or cross-hatched forceps for removing sutures, moving dressing or other drapes.
Commonly used thumb forceps include Adson forceps, Iris forceps and Foester forceps.
Locking forceps may be called clamps and are used to securely hold tissue. When they are used to control blood flow, they are called hemostats. When used to grasp and manipulate needles, they are called needle holders.
Spring scissors are uniquely designed for ambidextrous use. The action on these surgical scissors allows you to pinch the two blades together. The way they fit into your hand offers great visibility and incredible control.
Here are some tips to keep in mind when selecting spring scissors:
May be used with either left hand or right hand
Designed for neurosurgical, vascular and microsurgical uses
Cuts by shearing, reduces crushing of tissue
Fine tips are ideal for use in very restricted spaces
Curved tips are useful to avoid cutting of underlying tissue
Heavier blades are helpful when a thick tissue or vessel is to be cut
Length of the scissor tips can help with the depth of the incision:
Shorter tips for near-to-surface incision
Longer tips for deep incision
- WPI offers a broad category of infection control products to help you maintain a safe and compliant work area. Infection control products are designed to reduce the risk of disease contanimation from one person to another.
In our personal protective equipment line we offer a robust reusable face masks with disposable filters.
WPI also carries many sterilization cassettes, sterilization baskets and steriliazation trays which are perfect for sorting, sterilzing and storing surgical instruments and other small components.
As far as sterilizing surgical instruments and other PPE, you can see everything from autoclaves and ultrasonic cleaners to ultraviolet (UV) sterilizers.
When the threats of contaminents are a concern, take a look at the infection control products offered here.
- High level disinfection (HLD) removes most microbial life on surgical instuments, biosensors, etc. If instruments or equipment may be damaged by autoclaving, chemical disinfection may be used. Some chemicals that may be considered include:
Ortho-phthalaldehyde (OPA) like Rapicide for 5–12 minutes
Glutaraldehyde 2% for 20 minutes
Hydrogen peroxide 6%–7.5% for 20–30 minutes
Peracetic acid 0.2–0.35% for 5 minutes
Rapicide OPA/28 is our disinfectant of choice. It is the fastest disinfection time in the U.S. market and twice the reuse period of other OPA brands. Protect yourself by limiting your exposure to disinfectant chemicals. The low percentage of OPA and low vapor pressure reduces the irritation to your eyes, nose and throat, as compared to Glutaraldehyde.
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April 22, 2021The PUL-1000 is a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes. We will show you how to run a glass softening test. You should run the Softening Test when: You change the filament Lot numbers or capillary types change You create or modify a program The ambient environment changes Press the STOP key to quit any running program. The following window displays. Mount a glass capillary on the carriage. Press the START key to run the Glass Capillary Softening Test. The heating power increases gradually. The heating stops when the glass begins to move. Record the heating power. This is the baseline heating value for the glass type tested. It is a good starting point for the first stage of your program. If you have any questions, just give us a call. Buy Microelectrode Puller
April 22, 2021The PUL-1000 is a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes. Here are the resolutions for some common issues with the puller. Puller Stops If the puller stops in the middle of a pull and the unit beeps, one of two issues may be the culprit. The unit may be over heating. Allow the unit time to cool down before attempting to pull more glass. If a program fails in the middle of a pull, you may have exceeded the parameters of the unit. For example, when you add up the distance of travel for all the stages, you may have exceeded the maximum range of travel. Check your program. You may need to alter the heating or travel parameters to conform with the unit’s maximums. Glass Doesn’t Pull Well If the glass does not pull well, look at one of these possible causes. The parameter may not be set properly for the properties of the new glass. Run the Glass Softening Test to establish a new baseline heating parameter. The filament may be wearing out or may have broken. Install a new filament. Cannot Select a Program If you cannot select a program, the puller may be in Edit mode. It will not execute a program until you exit the Edit mode. Press the Exit key to exit the program editing mode without saving the program. Press Start to run your program. Unit Beeps If the unit beeps and the program fails to start, the carriages may be too far apart. Remove the glass, slide the carriages together and reposition the glass. If you have any questions, just give us a call.
April 22, 2021The PUL-1000 is a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes. Here we will see how to load a piece of glass into the carriage. The glass capillary is held by clamps mounted on two movable carriages. Both carriages synchronously slide as a program is executed. Manually slide the carriages together when you are loading the glass. To open the clamps, place your thumb under the clamp and depress the release button with your index finger. Slide the glass capillary into the groove from one side. Slide it through the center of heating filament onto the other
April 20, 2021by Benjamin Dubansky, PhD, Animal Physiologist/Researcher An Ussing Chamber is used when performing barrier studies across an excised epithelial tissue. Similar measurements can be made in well plates using an EVOM series TEER Measurement Meter, which is used for epithelial tissue that is grown to confluence in a cell culture well. While EVOM measurements are only qualitative, your throughput is much greater with an EVOM3. The EVOMs are portable and much more economical than an Ussing Chamber. Here Ben Dubansky, PhD explains the differences between EVOMs and Ussing chambers. The EVOM is a special kind of voltmeter for measuring electrical properties across a layer of cells in culture or a biological membrane. We are measuring TEER (TER) - Transepithelial Electrical Resistance. The