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- Microinjection processes use either metal microinjection needles or glass micropipettes to inject small liquid volumes. For example, genetic material may be inserted into a living cell, a drug introduced into an eye or brain, or fluid injected into a muscle. Typically, microinjection is performed under a microscope. A stereotaxic frame setup may be required.
Recently, WPI introduced its customizable Microinjection System with everything you need to get started. We can help you customize your system with many options and microinjection system accessories. The basic system includes:
Microinjection pump like the PV850 Microinjector with External Pressure Source
LED lighted microscope base with a PZMIII Stereo Microscope and an articulating mirror
WPI offers a variety of pumps along with special syringes, stereotaxic frames, glass capillaries and needles. The setup you choose depends on the size of your microinjection aliquots, the volume to be injected and the size of needle or glass tip you choose. In addition, we offer an electroporator for transfection procedures.
Whether you are working with Danio rerio (zebrafish), Xenopus, Drosophila or Caenorhabditis elegans, we can help you customize a microinjection system for your application.
- During the last 30 years, TEER measurements have become universally established as the most convenient, reliable and non-destructive method of evaluating and monitoring the growth of epithelial tissue culture in vitro. The confluence of the cellular monolayer is quickly determined by a sharp increase in TEER. TEER measurement technology, first introduced to tissue and cell biologist by WPI in the mid '80s has since been perfected and expanded to include a range of TEER related manual and automatic instrumentation.
For epithelial physiology studies, our Ussing systems offer a quick, effective means of making low-resistance electrical connections to the Ussing chamber without the need of long agar bridges. Ag/AgCl half-cells screw into short tubes which plug firmly into place in the chamber's luer ports, eliminating the inconvenience and expense of Calomel half-cells in open liquids.
- Better Imaging Results with Optical-Grade Glass Bottom Dishes
WPI's optical quality glass bottom FluoroDish Cell Culture dishes offer better imaging quality. There are several varieties that let life science researchers work with small sample volumes. This is necessary when researchers are working with expensive chemicals or experimental drugs. They are designed with the lowest access angle for easier insertion of a micropipette during cellular microinjection. They are perfect for live cell imaging and embryo research.
Cited in hundreds of reference articles, our optical grade, glass bottom dishes are unique in the marketplace and conform to strict quality control standards.
- Coating surgical instruments with a black ceramic adds a thin layer to the metal instrument, making the instrument harder and giving you greater precision. This anti-glare surface minimizes reflections off the surface of your instruments.
- Surgical tweezer, forceps, needle pullers – here's some information on selecting the proper forceps for your application.
Choosing Surgical Forceps
Surgical forceps may be broadly divided into two categories, thumb forceps (frequently called surgical tweezers or pinning forceps) and ring forceps (also called hemostats, hemostatic forceps and locking forceps).
Thumb forceps are spring forceps used by compression between your thumb and forefinger and are used for grasping, holding or manipulating body tissue. They are non-ratchet style. For example, you could use thumb forceps to hold or move tissue during surgery or to move dressings.
Hemostatic forceps are hinged forceps that look more like scissors. Hinged forceps may come with or without a "lock" for clamping.
Thumb forceps are available with a variety of tips. The tips may be flat, serrated, cupped, ringed, grooved, diamond dusted or have teeth. The tips may also be straight, curved or angled. See the images below. Serrated tweezers (thumb forceps) are designed for use with tissues. The serrations or teeth actually cause less damage than flat forceps, because it requires less pressure to maintain a firm grip. Use smooth or cross-hatched forceps for removing sutures, moving dressing or other drapes.
Commonly used thumb forceps include Adson forceps, Iris forceps and Foester forceps.
Locking forceps may be called clamps and are used to securely hold tissue. When they are used to control blood flow, they are called hemostats. When used to grasp and manipulate needles, they are called needle holders.
Spring scissors are uniquely designed for ambidextrous use. The action on these surgical scissors allows you to pinch the two blades together. The way they fit into your hand offers great visibility and incredible control.
Here are some tips to keep in mind when selecting spring scissors:
May be used with either left hand or right hand
Designed for neurosurgical, vascular and microsurgical uses
Cuts by shearing, reduces crushing of tissue
Fine tips are ideal for use in very restricted spaces
Curved tips are useful to avoid cutting of underlying tissue
Heavier blades are helpful when a thick tissue or vessel is to be cut
Length of the scissor tips can help with the depth of the incision:
Shorter tips for near-to-surface incision
Longer tips for deep incision
- WPI offers a broad category of infection control products to help you maintain a safe and compliant work area. Infection control products are designed to reduce the risk of disease contanimation from one person to another.
In our personal protective equipment line we offer a robust reusable face masks with disposable filters.
WPI also carries many sterilization cassettes, sterilization baskets and steriliazation trays which are perfect for sorting, sterilzing and storing surgical instruments and other small components.
As far as sterilizing surgical instruments and other PPE, you can see everything from autoclaves and ultrasonic cleaners to ultraviolet (UV) sterilizers.
When the threats of contaminents are a concern, take a look at the infection control products offered here.
- High level disinfection (HLD) removes most microbial life on surgical instuments, biosensors, etc. If instruments or equipment may be damaged by autoclaving, chemical disinfection may be used. Some chemicals that may be considered include:
Ortho-phthalaldehyde (OPA) like Rapicide for 5–12 minutes
Glutaraldehyde 2% for 20 minutes
Hydrogen peroxide 6%–7.5% for 20–30 minutes
Peracetic acid 0.2–0.35% for 5 minutes
Rapicide OPA/28 is our disinfectant of choice. It is the fastest disinfection time in the U.S. market and twice the reuse period of other OPA brands. Protect yourself by limiting your exposure to disinfectant chemicals. The low percentage of OPA and low vapor pressure reduces the irritation to your eyes, nose and throat, as compared to Glutaraldehyde.
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May 11, 2021by Carmen Raymond Stainless steel and polymers are two of the most popular material options for sterlization trays used in the pharmaceutical and medical industries. In terms of toughness, long shelf life and resistance to contaminant absorption, surgical stainless steel outperforms plastic trays, especially for medical usage. Stainless steel trays also have a lower total cost over time. Plastics can become discolored, can absorb fluids, have limited life shelf and are expensive over time. The CDC advises using a temperature between 121°C (250°F) to 132°C (270°F) for steam sterilization of surgical instruments. (Guideline for Disinfection and
May 10, 2021by Carmen Raymond Stainless steel sterilization baskets require a proper finish to increase the basket's versatility. The correct finish of stainless steel enchances it chemical resistance, handling characteristics and other key operational features. Let's take a look at the two most common finishes for stainless steel. Passivation Passivation is a corrosion-prevention treatment of stainless steel. This chemical process involves removing free iron from the metal's surface with citric acid or nitric acid. The process of passivation creates a thick protective oxide coating on the stainless steel, which makes it resistant to corrosive chemicals. The process of passivation cleans the stainless steel surface, removing all grease, oils and dirt. This results in a uniform, smooth appearance and finish. A smooth pasivation finish minimizes the chance of rust damage of your sterilization basket, surgical instrument or stainless steel product. It also eliminates extra maintenance steps to protect your investments. Electropolishing Electropolishing is a method of dissolving the surface layer of a substance using a temperature-controlled electrolyte bath and an electric current. Stainless steel has a smooth, featureless surface after it is electropolished. The process of electropolishing removes all the microscopic debris and defects that would usually adhere to the surface of the steel, and it leaves the material with a smooth, non-stick finish. Electropolishing improves "weldability" without compromising the surface's hardness or causing peeling or abrading. It also creates a smooth surface that prevents pollutants from hiding in microscopic pits. By removing sharp edges and burrs created by welds or other metal forming processes, electropolishing makes equipment safer for manual use. In any application, an electropolished finish reduces minor injuries, as well as the risk of infection from open cuts. When choosing sterlization baskets, consider the benefits of a proper finish. Both passivation and electropolishing make your surgical instrument baskets safer and easier to use and ensure a longer life of the product. See Sterilization Baskets & Trays
April 27, 2021The PUL-1000 is a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes. Here is how to run a program that will pull two identical microelectrodes. Loading a Program We will use the keypad to navigate through the display. Use the arrows to navigate to LOAD (00). The top and bottom arrows on the keypad navigate numbers in increments of 1. The left and right arrows on the keypad navigate numbers in increments of 10. Press ENTER. The number will start blinking. Use the arrows to navigate to the program number you would like load. Once you reach the desired program, press ENTER to run the program and pull your glass. Creating a New Program Use the arrows to navigate to STEP 1 - HEAT. Press ENTER. The number will start blinking. Use the arrows to set the desired value for the heat. Press ENTER. Navigate to STEP 1 – FORCE. Press ENTER. The number will start blinking. Use the arrows to set the desired value of force. Press ENTER. Navigate to STEP 1 – DISTANCE. Press ENTER. The number will start blinking. Use the arrows to set the desired value for the pulling distance. Press ENTER. If you are only using 1 stage, you do not need a delay. If you need more than one stage, set your delay and repeat the above for stage 2. Once you have successfully set all numbers of your desired settings, navigate to the SAVE (00). Press ENTER. The number will start blinking. Use the arrows to navigate to the program number where you want to save your new program. Once you reach the desired display press ENTER. Modifying an Existing Protocol
April 23, 2021The PUL-1000 is a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes. Here is a quick overview to get you started quickly. Quick Start Setup Plug the power cord into the wall outlet. An LED light on the power supply illuminated when the unit is connected to power. Plug the power cord into the rear panel of the PUL-1000. Then, the power switch on the side panel illuminates. Press the Power button to turn on the display. To open the glass capillary clamps, place your thumb under the clamp and depress the release button with your index finger. This allows the carriages to move freely. Push the carriages together toward the center. Install the silicone glass capillary