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- Microinjection processes use either metal microinjection needles or glass micropipettes to inject small liquid volumes. For example, genetic material may be inserted into a living cell, a drug introduced into an eye or brain, or fluid injected into a muscle. Typically, microinjection is performed under a microscope. A stereotaxic frame setup may be required.
Recently, WPI introduced its customizable Microinjection System with everything you need to get started. We can help you customize your system with many options and microinjection system accessories. The basic system includes:
Microinjection pump like the PV820 Pneumatic PicoPump
LED lighted microscope base with a PZMIII Stereo Microscope and an articulating mirror
WPI offers a variety of pumps along with special syringes, stereotaxic frames, glass capillaries and needles. The setup you choose depends on the size of your microinjection aliquots, the volume to be injected and the size of needle or glass tip you choose. In addition, we offer an electroporator for transfection procedures.
Whether you are working with Danio rerio (zebrafish), Xenopus, Drosophila or Caenorhabditis elegans, we can help you customize a microinjection system for your application.
- During the last 30 years, TEER measurements have become universally established as the most convenient, reliable and non-destructive method of evaluating and monitoring the growth of epithelial tissue culture in vitro. The confluence of the cellular monolayer is quickly determined by a sharp increase in TEER. TEER measurement technology, first introduced to tissue and cell biologist by WPI in the mid '80s has since been perfected and expanded to include a range of TEER related manual and automatic instrumentation.
For epithelial physiology studies, our Ussing systems offer a quick, effective means of making low-resistance electrical connections to the Ussing chamber without the need of long agar bridges. Ag/AgCl half-cells screw into short tubes which plug firmly into place in the chamber's luer ports, eliminating the inconvenience and expense of Calomel half-cells in open liquids.
- WPI's optical quality glass bottom FluoroDish Cell Culture dishes offer better imaging quality. There are several varieties that let life science researchers work with small sample volumes. This is necessary when researchers are working with expensive chemicals or experimental drugs. They are designed with the lowest access angle for easier insertion of a micropipette during cellular microinjection. They are perfect for live cell imaging and embryo research.
Cited in hundreds of reference articles, our optical grade, glass bottom dishes are unique in the marketplace and conform to strict quality control standards.
- Surgical tweezer, forceps, needle pullers – here's some information on selecting the proper forceps for your application.
Choosing Surgical Forceps
Surgical forceps may be broadly divided into two categories, thumb forceps (frequently called surgical tweezers or pinning forceps) and ring forceps (also called hemostats, hemostatic forceps and locking forceps).
Thumb forceps are spring forceps used by compression between your thumb and forefinger and are used for grasping, holding or manipulating body tissue. They are non-ratchet style. For example, you could use thumb forceps to hold or move tissue during surgery or to move dressings.
Hemostatic forceps are hinged forceps that look more like scissors. Hinged forceps may come with or without a "lock" for clamping.
Thumb forceps are available with a variety of tips. The tips may be flat, serrated, cupped, ringed, grooved, diamond dusted or have teeth. The tips may also be straight, curved or angled. See the images below. Serrated tweezers (thumb forceps) are designed for use with tissues. The serrations or teeth actually cause less damage than flat forceps, because it requires less pressure to maintain a firm grip. Use smooth or cross-hatched forceps for removing sutures, moving dressing or other drapes.
Commonly used thumb forceps include Adson forceps, Iris forceps and Foester forceps.
Locking forceps may be called clamps and are used to securely hold tissue. When they are used to control blood flow, they are called hemostats. When used to grasp and manipulate needles, they are called needle holders.
Spring scissors are uniquely designed for ambidextrous use. The action on these surgical scissors allows you to pinch the two blades together. The way they fit into your hand offers great visibility and incredible control.
Here are some tips to keep in mind when selecting spring scissors:
May be used with either left hand or right hand
Designed for neurosurgical, vascular and microsurgical uses
Cuts by shearing, reduces crushing of tissue
Fine tips are ideal for use in very restricted spaces
Curved tips are useful to avoid cutting of underlying tissue
Heavier blades are helpful when a thick tissue or vessel is to be cut
Length of the scissor tips can help with the depth of the incision:
Shorter tips for near-to-surface incision
Longer tips for deep incision
- WPI offers a broad category of infection control products to help you maintain a safe and compliant work area. Infection control products are designed to reduce the risk of disease contanimation from one person to another.
In our personal protective equipment line we offer a robust reusable face masks with disposable filters.
WPI also carries many sterilization cassettes, sterilization baskets and steriliazation trays which are perfect for sorting, sterilzing and storing surgical instruments and other small components.
As far as sterilizing surgical instruments and other PPE, you can see everything from autoclaves and ultrasonic cleaners to ultraviolet (UV) sterilizers.
When the threats of contaminents are a concern, take a look at the infection control products offered here.
- High level disinfection (HLD) removes most microbial life on surgical instuments, biosensors, etc. If instruments or equipment may be damaged by autoclaving, chemical disinfection may be used. Some chemicals that may be considered include:
Ortho-phthalaldehyde (OPA) like Rapicide for 5–12 minutes
Glutaraldehyde 2% for 20 minutes
Hydrogen peroxide 6%–7.5% for 20–30 minutes
Peracetic acid 0.2–0.35% for 5 minutes
Rapicide OPA/28 is our disinfectant of choice. It is the fastest disinfection time in the U.S. market and twice the reuse period of other OPA brands. Protect yourself by limiting your exposure to disinfectant chemicals. The low percentage of OPA and low vapor pressure reduces the irritation to your eyes, nose and throat, as compared to Glutaraldehyde.
Latest Posts See more
September 08, 2020[by Gabe Gonzalez] The PV850 Injector is designed to simplify intracellular injection and a variety of other microinjection tasks. The PV850 uses regulated air pressure for injecting cells with fluid. Injected volumes range from picoliters to nanoliters. The port supplies positive pressure for high-pressure ejection maximum of 8 7PSI. The PV850 Microinjector offers separate regulated compensation (back filling prevention) and ejection pressures with a precision timing circuit that switches from injection pressure to compensation pressure automatically. Timing, injection pressure and compensation pressure are adjusted independently using the intuitive touch-screen user interface. Time intervals can range from 2 seconds down to
September 08, 2020[by Gabe Gonzalez] The PV850 Injector is designed to simplify intracellular injection and a variety of other microinjection tasks. The PV850 uses regulated air pressure for injecting cells with fluid. Injected volumes range from picoliters to nanoliters. The port supplies positive pressure for high-pressure ejection maximum of 87 PSI. The PV850 Microinjector offers separate regulated compensation (back filling prevention) and ejection pressures with a precision timing circuit that switches from injection pressure to compensation pressure automatically. Timing, injection pressure and compensation pressure are adjusted independently using the intuitive touch-screen user interface. Time intervals can range from 2 seconds down to
September 03, 2020Designed to simplify intracellular injection and a variety of other microinjection tasks, WPI’s PicoPumps (PV830 and PV820) use precisely regulated pressures for securing cells and injecting them with fluid. Injected volumes range from picoliters to nanoliters. Separate ports supply positive and negative pressure—positive pressure for high-pressure ejection, and suction for supporting the cell or for filling the pipette from the tip. In this quick series, you can see how to setup a PicoPump. 1. What's Included with a PV Pump In this first video (#1) you can see all the components of the system as they unpack the system.