New Products See more
Popular Lab Supplies
- Microinjection processes use either metal microinjection needles or glass micropipettes to inject small liquid volumes. For example, genetic material may be inserted into a living cell, a drug introduced into an eye or brain, or fluid injected into a muscle. Typically, microinjection is performed under a microscope. A stereotaxic frame setup may be required.
Recently, WPI introduced its customizable Microinjection System with everything you need to get started. We can help you customize your system with many options and microinjection system accessories. The basic system includes:
Microinjection pump like the PV820 Pneumatic PicoPump
LED lighted microscope base with a PZMIII Stereo Microscope and an articulating mirror
WPI offers a variety of pumps along with special syringes, stereotaxic frames, glass capillaries and needles. The setup you choose depends on the size of your microinjection aliquots, the volume to be injected and the size of needle or glass tip you choose. In addition, we offer an electroporator for transfection procedures.
Whether you are working with Danio rerio (zebrafish), Xenopus, Drosophila or Caenorhabditis elegans, we can help you customize a microinjection system for your application.
Our free radical analyzers are used in biosensing experiments to detect oxygen, nitric oxide, carbon dioxide, hydrogen peroxide, hydrogen sulfide and glucose, using WPI ion-species specific biosensors. Our TBR series biosensing analyzers can be set to monitor the specific species of interest, and the 4-channel TBR4100 can monitor four biosensors simultaneously. While the TBR biosensing device is commonly used as a nitric oxide analyzer, it is perfect for real-time detection and measurement of a variety of redox-reactive species using the electrochemical (amperometric) detection principle. This series of optically isolated biosensing analyzers has ultra low noise and independently operated channels
- These products have been discontinued and are steeply discounted for a quick sale. Find a bargain today.
Latest Posts See more
March 02, 2020With the first approved human gene therapy trial in 19891 (Rosenberg, et.al.), gene therapy has come a long way in modern medicine and is making inroads in clinics and the market in general.2,3 2017 was an important year for gene therapy when Luxurna, the first human gene therapy drug for an inherited retinal dystrophy, was approved by Food and Drug Administration (USA).4 Now several drugs are undergoing clinical trials. With an estimated $11 billion (USD) market in the next 10 years, both clinical trials and the pharmaceutical industry are expected to benefit immensely from gene therapy.5 With improved use of viral vectors (Adeno-associated virus (AAV), adenovirus, lentivirus, retrovirus, HSV) or non-viral vectors, research and clinical trials on the development of therapeutic genes have witnessed great success. Viral vectors
February 25, 2020Nitric oxide (NO) is an essential signaling molecule and is known to play a significant role in a multitude of physiological systems including the central nervous system (CNS), the cardiovascular system, the gastrointestinal tract, the immune system, and the renal system. 1-5 However, being highly reactive, detection and quantification of NO is very difficult.6,7 It requires a sensor that is sensitive, selective to NO, and easy to calibrate. WPI’s Free Radical Analyzer (4-channel TBR4100 and single-channel TBR1025) and the LabTrax Data Acquisition System with the options o
January 24, 2020Microinjection is the process of transferring genetic materials into a living cell using glass micropipettes or metal microinjection needles. Glass micropipettes can be of various sizes with tip diameters ranging from 0.1 to 10 µm. DNA or RNA is injected directly into the cell’s nucleus. Microinjection has been successfully used with large frog eggs, mammalian cells, mammalian embryos, plants and tissues. Microinjection has been expensive, can be a slow process and requires skilled personnel, but new technologies are making it even more reliable, repeatable and affordable. Pronuclear injection, inserting DNA or RNA into the nucleus of a fertilized oocyte to create transgenic organisms, lets researchers study the role of particular genes. This horizontal gene transfer can insert genetic material from the same species or a
December 20, 2019The purpose of this article is to describe a new cleaning procedure for thoroughly cleaning WPI flowcells, including Liquid Waveguide Capillary Cells (LWCCs), UltraPath flowcells and optical cuvettes. The image (right) shows a 3000 series LWCC with a MiniStar pump and the LWCC injection system placed on top of it. Preparation of Chemicals All chemical reagents should be of at least ACS-Grade, preferably HPLC-Grade. This procedure involves the use of caustic and flammable reagents. Consult the manufacturer’s MSDS for necessary safety precautions. Cleaning Solution #1: 0.5M Potassium Hydroxide in 100% Ethanol (e.g.: 7 g KOH in 250mL EtOH). After thoroughly mixing, filter the solution through a 20µm pore size filter. Cleaning Solution #2: 100% Methanol, HPLC grade Cleaning So