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- Microinjection processes use either metal microinjection needles or glass micropipettes to inject small liquid volumes. For example, genetic material may be inserted into a living cell, a drug introduced into an eye or brain, or fluid injected into a muscle. Typically, microinjection is performed under a microscope. A stereotaxic frame setup may be required.
Recently, WPI introduced its customizable Microinjection System with everything you need to get started. We can help you customize your system with many options and microinjection system accessories. The basic system includes:
Microinjection pump like the PV820 Pneumatic PicoPump
LED lighted microscope base with a PZMIII Stereo Microscope and an articulating mirror
WPI offers a variety of pumps along with special syringes, stereotaxic frames, glass capillaries and needles. The setup you choose depends on the size of your microinjection aliquots, the volume to be injected and the size of needle or glass tip you choose. In addition, we offer an electroporator for transfection procedures.
Whether you are working with Danio rerio (zebrafish), Xenopus, Drosophila or Caenorhabditis elegans, we can help you customize a microinjection system for your application.
- During the last 30 years, TEER measurements have become universally established as the most convenient, reliable and non-destructive method of evaluating and monitoring the growth of epithelial tissue culture in vitro. The confluence of the cellular monolayer is quickly determined by a sharp increase in TEER. TEER measurement technology, first introduced to tissue and cell biologist by WPI in the mid '80s has since been perfected and expanded to include a range of TEER related manual and automatic instrumentation.
For epithelial physiology studies, our Ussing systems offer a quick, effective means of making low-resistance electrical connections to the Ussing chamber without the need of long agar bridges. Ag/AgCl half-cells screw into short tubes which plug firmly into place in the chamber's luer ports, eliminating the inconvenience and expense of Calomel half-cells in open liquids.
- WPI's optical quality glass bottom FluoroDish Cell Culture dishes offer better imaging quality. There are several varieties that let life science researchers work with small sample volumes. This is necessary when researchers are working with expensive chemicals or experimental drugs. They are designed with the lowest access angle for easier insertion of a micropipette during cellular microinjection. They are perfect for live cell imaging and embryo research.
Cited in hundreds of reference articles, our optical grade, glass bottom dishes are unique in the marketplace and conform to strict quality control standards.
- Coating surgical instruments with a black ceramic adds a thin layer to the metal instrument, making the instrument harder and giving you greater precision. This anti-glare surface minimizes reflections off the surface of your instruments.
- Surgical tweezer, forceps, needle pullers – here's some information on selecting the proper forceps for your application.
Choosing Surgical Forceps
Surgical forceps may be broadly divided into two categories, thumb forceps (frequently called surgical tweezers or pinning forceps) and ring forceps (also called hemostats, hemostatic forceps and locking forceps).
Thumb forceps are spring forceps used by compression between your thumb and forefinger and are used for grasping, holding or manipulating body tissue. They are non-ratchet style. For example, you could use thumb forceps to hold or move tissue during surgery or to move dressings.
Hemostatic forceps are hinged forceps that look more like scissors. Hinged forceps may come with or without a "lock" for clamping.
Thumb forceps are available with a variety of tips. The tips may be flat, serrated, cupped, ringed, grooved, diamond dusted or have teeth. The tips may also be straight, curved or angled. See the images below. Serrated tweezers (thumb forceps) are designed for use with tissues. The serrations or teeth actually cause less damage than flat forceps, because it requires less pressure to maintain a firm grip. Use smooth or cross-hatched forceps for removing sutures, moving dressing or other drapes.
Commonly used thumb forceps include Adson forceps, Iris forceps and Foester forceps.
Locking forceps may be called clamps and are used to securely hold tissue. When they are used to control blood flow, they are called hemostats. When used to grasp and manipulate needles, they are called needle holders.
Spring scissors are uniquely designed for ambidextrous use. The action on these surgical scissors allows you to pinch the two blades together. The way they fit into your hand offers great visibility and incredible control.
Here are some tips to keep in mind when selecting spring scissors:
May be used with either left hand or right hand
Designed for neurosurgical, vascular and microsurgical uses
Cuts by shearing, reduces crushing of tissue
Fine tips are ideal for use in very restricted spaces
Curved tips are useful to avoid cutting of underlying tissue
Heavier blades are helpful when a thick tissue or vessel is to be cut
Length of the scissor tips can help with the depth of the incision:
Shorter tips for near-to-surface incision
Longer tips for deep incision
- WPI offers a broad category of infection control products to help you maintain a safe and compliant work area. Infection control products are designed to reduce the risk of disease contanimation from one person to another.
In our personal protective equipment line we offer a robust reusable face masks with disposable filters.
WPI also carries many sterilization cassettes, sterilization baskets and steriliazation trays which are perfect for sorting, sterilzing and storing surgical instruments and other small components.
As far as sterilizing surgical instruments and other PPE, you can see everything from autoclaves and ultrasonic cleaners to ultraviolet (UV) sterilizers.
When the threats of contaminents are a concern, take a look at the infection control products offered here.
- High level disinfection (HLD) removes most microbial life on surgical instuments, biosensors, etc. If instruments or equipment may be damaged by autoclaving, chemical disinfection may be used. Some chemicals that may be considered include:
Ortho-phthalaldehyde (OPA) like Rapicide for 5–12 minutes
Glutaraldehyde 2% for 20 minutes
Hydrogen peroxide 6%–7.5% for 20–30 minutes
Peracetic acid 0.2–0.35% for 5 minutes
Rapicide OPA/28 is our disinfectant of choice. It is the fastest disinfection time in the U.S. market and twice the reuse period of other OPA brands. Protect yourself by limiting your exposure to disinfectant chemicals. The low percentage of OPA and low vapor pressure reduces the irritation to your eyes, nose and throat, as compared to Glutaraldehyde.
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February 25, 2021NanoFil™ is a unique low volume syringe developed for improved microinjection in mice and other small animals. Using a UMP3 UltraMicroPump combined with the syringe allows you to make repeatable injections in the nanoliter range. Here we'll show you how to install a NanoFil Syringe on the UMP3 UltraMicroPump. NanoFil syringes are 10 µL and 100 µL syringes which can be installed on a UMP3 UltraMicroPump to give you repeatable injections in the nanoliter range. We will use the collar of the NanoFil syringe and the plunger as the two points to anchor the syringe to the pump.
February 25, 2021NanoFil™ is a unique low volume syringe developed for improved microinjection in mice and other small animals. RPE and IO kits are specially designed for “hands-free” and oil-free sub-microliter injection. Originally developed for retinal pigment epithelium and intraocular injection, they can also be used for other applications. The NanoFil syringe system utilizes a seal system that is based on a 0.46 mm shank diameter. Any needle or tubing with an outside diameter of 0.46 mm should fit the NanoFil’s seal system. Here is how to install your needle in a way that prevents damage to the gasket and keeps your needles straight.
February 25, 2021NanoFil™ is a unique low volume syringe developed for improved microinjection in mice and other small animals. Here we'll show you how to install your needle in a way that prevents damage to the gasket and keeps your needles straight. The NanoFil syringe system utilizes a seal system that is based on a 0.46 mm shank diameter. Any needle or tubing with an outside diameter of 0.46 mm should fit the NanoFil’s seal system. Here is how to install your needle in a way that prevents damage to the gasket and keeps your needles straight. PRO TIP: Always use surgical gloves to prevent the oils from your skin from affecting the
February 25, 2021NanoFil™ is a unique low volume syringe developed for improved microinjection in mice and other small animals. During our field trials, the 35-gauge needle was scientists’ most popular and preferred NanoFil tip. The combination of its strength, length, durability and clogging resistance creates a solid balance. The 33-gauge tip is similar to Hamilton's 7762 and 7803 series removable needles in both tip length and outer diameter. However, our beveled tip version is shorter, more durable, and penetrates better due to the special tri-surface grinding technique. 33-gauge tips used to be the smallest size sold, but our 35-gauge tip is much better for injections involving small ani