Microinjection in Zebrafish Otocytes

Researchers at the University of Michigan are using WPI's PV820 for injecting a morpholino solution into the lumen of the otic vesicle of 1-day old zebrafish embryos.  Then, they use electroporation to introduce mif and mif-like morpholinos into the developing inner ear tissues.

Microinjection in Zebrafish Oocytes

Check out the JOVE video to see:

  • How to make electrodes for electroporation
  • How to setup the electroporation
  • How to inject the morpholinos
  • Examine some of the results

More Info



JOVE videoHolmes, K. E., Wyatt, M. J., Shen, Y., Thompson, D. A., Barald, K. F. "Direct Delivery of MIF Morpholinos Into the Zebrafish Otocyst by Injection and Electroporation Affects Inner Ear Development". J. Vis. Exp. (47), e2466, doi:10.3791/2466 (2011).

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