This website uses cookies to ensure you get the best experience on our website.
Read more
You've placed your trust in our TEER measurement line for nearly 40 years as the premiere innovator and
manufacturer of EVOM™ meters, REMS, and Millicell ERS2. Learn more about the gold standard in TEER
technology that has been cited in over 16,000 peer-reviewed research articles.
How EVOM™ Technology Works
Electrical resistance (i.e., TEER) of a cellular layer is the inverse presentation of the electrical
conductance through the cellular layer. A high TEER value of the cellular layer is indicative of an intact cellular monolayer and suggests low or restricted permeability of ions and molecules (i.e., low conductance). Similarly, a decrease in the TEER value suggests a compromised barrier function and indicates increased permeability. Tissue permeability studies require a confluent cellular layer, and TEER measurement is generally used to confirm the formation of a confluent monolayer.
Initially, 24 hours after cell seeding transwell, TEER values are generally low, because the current passes can pass easily between the cells. Over time, the cells multiply and start covering the gaps. Finally, a confluent cellular monolayer is formed. At that point, the permeable membrane is fully covered with cells and does not allow easy passage of electrical current. This results in a high TEER value.
TEER Measurement of Leaky & Tight Cell Types
TEER values of confluent cellular monolayers can vary depending on the cell type. Monolayers of certain cell
types (e.g., cell type A), which normally show low TEER values, generally have relatively leaky tight junctions. Monolayers of other cell types (e.g., cell type B) show high TEER values, and these cell types are known to have tight junctions. Ions and molecules are known to pass rather easily across leaky cellular layers as compared to tighter cellular layers. The presence of more transcellular ion channels on cells can further allow easier flow of ions or electrical current through the transcellular pathway, which can additionally lower TEER values.
Cell type A allows greater amounts of current and ions to pass between the cells and yields a low TEER value. With its tighter junctions, cell monolayers of type B cells will show a higher TEER value. While both monolayers are confluent, the TEER resistance values can be markedly different based on the nature of the cells themselves.
Related Applications