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TEER

  1. What's the Difference between Hanging and Standing Cell Culture Inserts used in EndOhm Chambers
    February 22, 2019
    WPI EndOhm chambers are used with WPI's EVOM2 meter for making TEER (transepithelial electrical resistance) measurements. Here Subhra shows the difference between hanging cell culture inserts and standing cell culture inserts.
  2. Prevent Damage to Snapwell Inserts by Using STX3 Electrode
    February 21, 2019
    The WPI EVOM2 meter is used to measure TEER (transepithelial electrical resistance) in cell culture inserts. The STX2 electrodes with a static length may cause damage to Snapwell inserts. Here Subhra demonstrates how to use the adjustable STX3 electrode to prevent damage to your cell culture inserts.
  3. Choosing an EndOhm Chamber Compatible with your Cell Culture Inserts
    February 21, 2019
    WPI's EndOhm chambers are used with WPI's EVOM2 meter to measure TEER (transepithelial electrical resistance) in cell culture inserts. Three chamber sizes are available for 6-well plates, 12-well plates and 24-well plates. Here Subhra demonstrates how to choose an EndOhm chamber to match the cell culture inserts you are using.
  4. Resurfacing Electrodes in a WPI EndOhm Chamber
    February 21, 2019
    WPI EndOhm chambers are used with WPI's EVOM2 meter for making TEER (transepithelial electrical resistance) measurements. After heavy use your background resistance measurement may start to rise. Your electrodes may need to be resurfaced to remove buildup of proteins, sugars and biological materials. Here Subhra demonstrates how to clean and resurface your EndOhm chambers.
  5. How to Set the Gap on your WPI EndOhm Chambers
    February 21, 2019
    WPI EndOhm chambers are used with WPI's EVOM2 meter for making TEER (transepithelial electrical resistance) measurements. Each EndOhm chamber comes with a "spacer" disk for calibrating the gap between the two chamber electrodes. A consistent gap ensures reliable measurements. Here Subhra shows how to calibrate your chambers.
  6. How To Select Electrodes For Making TEER Measurements
    November 28, 2018
    Selection of Electrodes for TEER Measurements Transepithelial electrical resistance (TEER), also referred as the transepithelial resistance (TER) is used to monitor cellular health. TEER is comprised of measurements of the transcellular pathway (i.e., resistance due to an individual cell) and paracellular pathway (i.e., resistance due to the formation of the cellular junctions). TEER is commonly used to monitor cellular confluence. TEER values can indicate changes in the cellular monolayer permeability, showing the monolayer barrier function of cells such as, endothelial (brain microvessel) and epithelial (alveolar, kidney, and intestinal) cells. High TEER values generally reflect tighter cellular monolayers or cellular junctions (Lewis 1996, Matter and Balda 2003, Denker and Sabath 2011). A few major benefits of WPI TEER measurement systems are described below. The TEER values (electrophysiological analysis) can be combined with other analysis methods to further understand
  7. Monitor the Growth of Epithelial Cells in Vitro Manually
    November 20, 2015
    For small batches of cell culture Are you ready to perform experiments involving a cultured cell monolayer? If your monolayer has not reached confluence, you may have holes or gaps in the monolayer that would prevent collection of valid experimental data. When confluence is reached, the electrical resistance across the monolayer peaks and then plateaus. The EVOM2 meter was designed specifically for this application. The EVOM2 is the ideal meter for use when you are making manual TEER measurements on small batches of cell cultures and do not require a high throughput system. EVOM2 - Perfect for TEER The EVOM2 was designed to measure TEER resistance without damaging the epithelial cells. It passes a constant current
  8. Setting Up Your Automated TEER Measurement Robot
    April 14, 2015
    Now that your REMS system has arrived, are you trying to figure out how to set it up? Check out this 5-minute video where we show you how, step-by-step. More Info
  9. FAQs about TEER Measurement
    February 02, 2015
    Here are some frequently asked questions (FAQ) about TEER measurement using an EVOM2. Are the electrical resistance and transepithelial electrical resistance (TEER) the same thing? What is an EndOhm chamber? How is an EVOM2 used for measuring confluence? Why use an EndOhm instead of a STX? How do I clean my electrode? What about electrode preconditioning? Can you give me a simple data acquisition system for TEER? What are the TEER measurement challenges that I may encounter? Can you suggest some experimental parameters that can be controlled to obtain consistent TEER results?
  10. Recording TEER Measurements from an EVOM2
    November 11, 2014
    The EVOM2 is the classic, hand-held instrument for making Trans Epithelial Electrical Resistance (TEER) measurements. The REMS system adds a robot and data recording for automated TEER measurements. There may be times when you don't have a robot, but would still like to have the data recording capabilities of the REMS system. With a little ingenuity, you can do just that. Here, we will show you how you can add data recording to your standard EVOM2
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