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TEER

  1. Why Choose an EVOM3 Over an EVOM2 for TEER Measurement
    May 15, 2020
    After 12 years, the next generation of the EVOM is born. Used for measuring Transepithelial Electrical Resistance and monitoring the confluence of cell monolayers in culture well plates the EVOM3 TEER Measurement System enables researchers to carry out experiments more efficiently by improving the workflow, and increasing the stability and accuracy of readings over that of the EVOM2.  Here are six of the EVOM3 improvements.    
  2. 7 Reasons to Love the New EVOM3 for TEER Measurement
    May 15, 2020
    The NEW EVOM3 Epithelial Volt/Ohm Meter delivers improved workflow efficiency, increased stability and more repeatable measurements than traditional Trans Epithelial Electrical Resistance (TEER) meters. Here are seven things you will love about the new EVOM3.     1. It has an intuitive Touchscreen Display Providing users with vital feedback during experimental measurements, the EVOM3’s large touch screen offers a range of informational v
  3. Significance of Transepithelial/ Transendothelial Electrical Resistance (TEER) Measurement in Lung In vitro Models 
    April 13, 2020
      Epithelial and endothelial cells are known for their barrier function like selective permeability. Lung tissue is comprised of epithelial cellular layers, residing adjacent to endothelial layers and allowing exchange of oxygen and carbon dioxide between lung and blood. Currently, the biomedical research community’s major focus area is to understand the details of the infection caused by this novel coronavirus (2019-nCoV) or by similar viruses. This new virus can disrupt the lung’s normal cleaning ability to get rid of foreign particles in the lung, and the selective permeability or barrier function of the lung may become severely impaired. The adverse physiological effect may be sustained once the inflammatory cascade is initiated. Researchers in collaboration with clinicians are trying to develop vaccines and drugs to
  4. 7 Reasons to Love the NEW EndOhm Chambers
    May 02, 2019
    EndOhm Chambers Re-engineered EndOhm chambers are designed for making accurate TEER measurement of epithelial and endothelial cell cultures. The design keeps the top and bottom electrodes at a fixed gap and maintains the sample (cell culture insert) in a centered position inside. This design minimizes the variability associated with electrode positioning and gap, making EndOhm chambers more accurate and capable of generating reproducible results. WPI made some recent upgrades to the EndOhm chambers. The NEW EndOhm Chamber is made of GLASS, making it easier to clean
  5. What's the Difference between Hanging and Standing Cell Culture Inserts used in EndOhm Chambers
    February 22, 2019
    WPI EndOhm chambers are used with WPI's EVOM2 meter for making TEER (transepithelial electrical resistance) measurements. Here Subhra shows the difference between hanging cell culture inserts and standing cell culture inserts.
  6. How to Set the Gap on your WPI EndOhm Chambers
    February 21, 2019
    WPI EndOhm chambers are used with WPI's EVOM2 meter for making TEER (transepithelial electrical resistance) measurements. Each EndOhm chamber comes with a "spacer" disk for calibrating the gap between the two chamber electrodes. A consistent gap ensures reliable measurements. Here Subhra shows how to calibrate your chambers.
  7. Resurfacing Electrodes in a WPI EndOhm Chamber
    February 21, 2019
    WPI EndOhm chambers are used with WPI's EVOM2 meter for making TEER (transepithelial electrical resistance) measurements. After heavy use your background resistance measurement may start to rise. Your electrodes may need to be resurfaced to remove buildup of proteins, sugars and biological materials. Here Subhra demonstrates how to clean and resurface your EndOhm chambers.
  8. Choosing an EndOhm Chamber Compatible with your Cell Culture Inserts
    February 21, 2019
    WPI's EndOhm chambers are used with WPI's EVOM2 meter to measure TEER (transepithelial electrical resistance) in cell culture inserts. Three chamber sizes are available for 6-well plates, 12-well plates and 24-well plates. Here Subhra demonstrates how to choose an EndOhm chamber to match the cell culture inserts you are using.
  9. Prevent Damage to Snapwell Inserts by Using STX3 Electrode
    February 21, 2019
    The WPI EVOM2 meter is used to measure TEER (transepithelial electrical resistance) in cell culture inserts. The STX2 electrodes with a static length may cause damage to Snapwell inserts. Here Subhra demonstrates how to use the adjustable STX3 electrode to prevent damage to your cell culture inserts.
  10. How To Select Electrodes For Making TEER Measurements
    November 28, 2018
    Selection of Electrodes for TEER Measurements Transepithelial electrical resistance (TEER), also referred as the transepithelial resistance (TER) is used to monitor cellular health. TEER is comprised of measurements of the transcellular pathway (i.e., resistance due to an individual cell) and paracellular pathway (i.e., resistance due to the formation of the cellular junctions). TEER is commonly used to monitor cellular confluence. TEER values can indicate changes in the cellular monolayer permeability, showing the monolayer barrier function of cells such as, endothelial (brain microvessel) and epithelial (alveolar, kidney, and intestinal) cells. High TEER values generally reflect tighter cellular monolayers or cellular junctions (Lewis 1996, Matter and Balda 2003, Denker and Sabath 2011). A few major benefits of WPI TEER measurement systems are described below. The TEER values (electrophysiological analysis) can be combined with other analysis methods to further understand
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