1. All Topics
  2. Videos
  3. Testimonial
  4. Virus Research
  5. 900A Videos

Epithelial Physiology

  1. Why Choose an EVOM3 Over an EVOM2 for TEER Measurement
    May 15, 2020
    After 12 years, the next generation of the EVOM is born. Used for measuring Transepithelial Electrical Resistance (TEER) and monitoring the confluence of cell monolayers in culture well plates the EVOM3 TEER Measurement System enables researchers to carry out experiments more efficiently by improving the workflow, and increasing the stability and accuracy of readings over that of the EVOM2.  Here are six of the EVOM3 improvements.    
  2. 7 Reasons to Love the New EVOM3 for TEER Measurement
    May 15, 2020
    The NEW EVOM3 Epithelial Volt/Ohm Meter delivers improved workflow efficiency, increased stability and more repeatable measurements than traditional Trans Epithelial Electrical Resistance (TEER) meters. Here are seven things you will love about the new EVOM3.     1. It has an intuitive Touchscreen Display Providing users with vital feedback during experimental measurements, the EVOM3’s large touch screen offers a range of informational views. The new graphical displays for trend analysis and measurement values helps you deliver a simple, stepwise methodology during experimental measurements. The touch screen interface has an intuitive, easy-to-use menu for configuration and calibration that is designed to eliminate human errors and reduce experimental processing time. 2. EVOM3 offers Auto Data Logging Eliminating the need to log data by hand, the EVOM3 writes the resistance or voltage information to a USB drive in CSV format for easy transfer to spreadsheets and data analysis programs. 3. With the EVOM3 you get Hands Free Recording When used with the footswitch, it enables hands-free recording of measurements. 4. You can make Quick, Easy, Reliable Readings At the heart of the EVOM3 is our latest processor and circuitry, providing users with quick, easy and reliable readings due to its fast stabilization, automatic twenty times sampling average and low noise d
  3. Significance of Transepithelial/ Transendothelial Electrical Resistance (TEER) Measurement in Lung In vitro Models 
    April 13, 2020
      Epithelial and endothelial cells are known for their barrier function like selective permeability. Lung tissue is comprised of epithelial cellular layers, residing adjacent to endothelial layers and allowing exchange of oxygen and carbon dioxide between lung and blood. Currently, the biomedical research community’s major focus area is to understand the details of the infection caused by this novel coronavirus (2019-nCoV) or by similar viruses. This new virus can disrupt the lung’s normal cleaning ability to get rid of foreign particles in the lung, and the selective permeability or barrier function of the lung may become severely impaired. The adverse physiological effect may be sustained once the inflammatory cascade is initiated. Researchers in collaboration with clinicians are trying to develop vaccines and drugs to
  4. 7 Reasons to Love the NEW EndOhm Chambers
    May 02, 2019
    EndOhm Chambers Re-engineered EndOhm chambers are designed for making accurate TEER measurement of epithelial and endothelial cell cultures. The design keeps the top and bottom electrodes at a fixed gap and maintains the sample (cell culture insert) in a centered position inside. This design minimizes the variability associated with electrode positioning and gap, making EndOhm chambers more accurate and capable of generating reproducible results. WPI made some recent upgrades to the EndOhm chambers. The NEW EndOhm Chamber is made of GLASS, making it easier to clean and more crack resistant. The chamber can be cleaned with ethanol, isopropanol, bleach, etc. The chambers are still compatible with the original EVOM and EVOM2™ meters. The apical electrode height is adjustable. The chamber comes with a spacer disk. (Video) The crystal clear glass chamber allows better visualization of apical electrode for accurate sample positioning. A new insert holder on the top of the chambers has tri-supports for three leg inserts. This helps keep your cell culture wells in the proper position (centered) when you are making measurements. Three sizes cover a range of well cup sizes from a variety of manufacturers. Make Precise Measurements Using WPI’s EVOM2™ resistance meter/epithelial volt meter, Endohm chambers provide reproducible resistance measurements of endothelial and epithelial monolayers grown in culture cups or inserts. Transfer cups from their culture wells to the Endohm chamber for measurement rather than using hand-held electrodes. The chamber and the cap each contain a pair of concentric electrodes: a voltage-sensing silver/silver chloride pellet in the center plus an annular current electrode. The height of the top electrode can be adjusted to fit cell culture cups from different manufacturers. Endohm’s circular disc electrodes, situated above and beneath the membrane, allow a more uniform current density across the membrane than a system using the STX2 electrodes. Compared with other resistance measurement methods, Endohm with EVOM2™ offers a much more convenient and economic solution to monitor the health of cellular monolayers in terms of TEER values. The EndOhm chamber (combined with the EVOM2™) applies an alternating form of current,  and the chamber design guides the current, creating a more uniform current density across the sample. The EndOhm and EVOM2™ system can produce reliable read outs, because it eliminates most common errors associated with electrode polarization and membrane capacitance. Endohm together with EVOM2™ offers an accurate and economical system to measure resistance of cellular monolayers.  More Info
  5. What's the Difference between Hanging and Standing Cell Culture Inserts used in EndOhm Chambers
    February 22, 2019
    WPI EndOhm chambers are used with WPI's EVOM2 meter for making TEER (transepithelial electrical resistance) measurements. Here Subhra shows the difference between hanging cell culture inserts and standing cell culture inserts.
  6. How to Set the Gap on your WPI EndOhm Chambers
    February 21, 2019
    WPI EndOhm chambers are used with WPI's EVOM2 meter for making TEER (transepithelial electrical resistance) measurements. Each EndOhm chamber comes with a "spacer" disk for calibrating the gap between the two chamber electrodes. A consistent gap ensures reliable measurements. Here Subhra shows how to calibrate your chambers.
  7. Resurfacing Electrodes in a WPI EndOhm Chamber
    February 21, 2019
    WPI EndOhm chambers are used with WPI's EVOM2 meter for making TEER (transepithelial electrical resistance) measurements. After heavy use your background resistance measurement may start to rise. Your electrodes may need to be resurfaced to remove buildup of proteins, sugars and biological materials. Here Subhra demonstrates how to clean and resurface your EndOhm chambers.
  8. Choosing an EndOhm Chamber Compatible with your Cell Culture Inserts
    February 21, 2019
    WPI's EndOhm chambers are used with WPI's EVOM2 meter to measure TEER (transepithelial electrical resistance) in cell culture inserts. Three chamber sizes are available for 6-well plates, 12-well plates and 24-well plates. Here Subhra demonstrates how to choose an EndOhm chamber to match the cell culture inserts you are using.
  9. Prevent Damage to Snapwell Inserts by Using STX3 Electrode
    February 21, 2019
    The WPI EVOM2 meter is used to measure TEER (transepithelial electrical resistance) in cell culture inserts. The STX2 electrodes with a static length may cause damage to Snapwell inserts. Here Subhra demonstrates how to use the adjustable STX3 electrode to prevent damage to your cell culture inserts.
  10. Monitor the Growth of Epithelial Cells in Vitro Manually
    November 20, 2015
    For small batches of cell culture Are you ready to perform experiments involving a cultured cell monolayer? If your monolayer has not reached confluence, you may have holes or gaps in the monolayer that would prevent collection of valid experimental data. When confluence is reached, the electrical resistance across the monolayer peaks and then plateaus. The EVOM2 meter was designed specifically for this application. The EVOM2 is the ideal meter for use when you are making manual TEER measurements on small batches of cell cultures and do not require a high throughput system. EVOM2 - Perfect for TEER The EVOM2
Page
©Copyright 2021. World Precision Instruments. All rights reserved.

Apply for Tax Exempt Status
WPI collects tax in AL, AZ, CA, CO, CT, DC, FL, GA, IL, IN, MA, ME, MD, MI, MN, MO, NC, NV, NJ, NY, OH, OK, PA, SC, TN, TX, VA, VT, WA and WI