Laboratory Equipment Blog
The use of fluorescence for sensing and imaging of the cellular signaling pathways has emerged as an indispensable tool in modern physiology, providing dynamic information of quantity and localization of the molecules of interest. Using appropriate indicator dyes, molecules alter their fluorescent characteristics in response to ion binding or membrane integration, so that the optical signal from the indicator can be measured to monitor the amplitude and the time course of various metal ions like Na+, K+, Mg2+ and Ca2+, as well as pH and membrane potential, in cellular compartments.
Absorption of light correlates to the energy of a photon that is taken-up by electrons of the substance atom. The electromagnetic energy is transformed into internal energy of the absorbent substance. The absorbance of a substance quantifies how much of the incident light is absorbed by it (instead of being reflected or refracted). Precise measurements of the absorbance at many wavelengths allow the identification of a substance via absorption spectroscopy, where a sample is illuminated from one side, and the intensity of the light that exits from the sample in every direction is measured (see Fig. 1). A few examples of absorption are ultraviolet–visible (UV-Vis) spectroscopy or infrared (IR) spectroscopy.
The use of fluorescent probes in cell physiology has emerged as indispensable tool in the analysis of cell functioning over recent years. The physics underlying fluorescence is illustrated by the electronic-state diagram (so-called Jablonski diagram, see Fig. 1), showing the three-stage process to create the fluorescent signal (Excitation - Excited/State Lifetime - Fluorescence Emission) in a fluorophore/indicator and simplified described below.
The head of WPI's biosensing division has published over 350 paper in peer-reviewed journals, authored 8 books and hold 65 patents!
World Precision Instruments was selected to receive the FVMA (Florida Veterinary Medical Association) Industry Award for 2017.
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