Laboratory Equipment Blog
Selecting surgical instruments for a procedure? Get answers to your questions here and suggestions for appropriate instruments.
- What procedure are you performing?
- What is the size of your subject?
- How often will the instrument be used?
In this article we will consider some of these factors and offer a few tips for selecting an appropriate pair of scissors, tweezers and forceps.
Surgical instruments are crucial in your research, and proper cleaning, handling and storage will ensure your instruments last for many years to come. Our 11x17 Instrument Care Poster is suitable for printing. Download your free copy of the Instrument Care Poster so you can post the best practices for instrument cleaning in your laboratory.
The use of fluorescence for sensing and imaging of the cellular signaling pathways has emerged as an indispensable tool in modern physiology, providing dynamic information of quantity and localization of the molecules of interest. Using appropriate indicator dyes, molecules alter their fluorescent characteristics in response to ion binding or membrane integration, so that the optical signal from the indicator can be measured to monitor the amplitude and the time course of various metal ions like Na+, K+, Mg2+ and Ca2+, as well as pH and membrane potential, in cellular compartments.
Absorption of light correlates to the energy of a photon that is taken-up by electrons of the substance atom. The electromagnetic energy is transformed into internal energy of the absorbent substance. The absorbance of a substance quantifies how much of the incident light is absorbed by it (instead of being reflected or refracted). Precise measurements of the absorbance at many wavelengths allow the identification of a substance via absorption spectroscopy, where a sample is illuminated from one side, and the intensity of the light that exits from the sample in every direction is measured (see Fig. 1). A few examples of absorption are ultraviolet–visible (UV-Vis) spectroscopy or infrared (IR) spectroscopy.
The use of fluorescent probes in cell physiology has emerged as indispensable tool in the analysis of cell functioning over recent years. The physics underlying fluorescence is illustrated by the electronic-state diagram (so-called Jablonski diagram, see Fig. 1), showing the three-stage process to create the fluorescent signal (Excitation - Excited/State Lifetime - Fluorescence Emission) in a fluorophore/indicator and simplified described below.
The EVOM2 is the ideal meter for use when you are making manual TEER measurements on small batches of cell cultures and do not require a high throughput system.