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Pullers

  1. How to Pull Glass using the PUL-1000 Micropipette Puller
    April 27, 2021
    The PUL-1000 is a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes. Here is how to run a program that will pull two identical microelectrodes.   Loading a Program   We will use the keypad to navigate through the display. Use the arrows to navigate to LOAD (00). The top and bottom arrows on the keypad navigate numbers in increments of 1. The left and right arrows on the keypad navigate numbers in increments of 10. Press ENTER. The number will start blinking. Use the arrows to navigate to the program number you would like load. Once you reach the desired program, press ENTER to run the program and pull your glass.   Creating a New Program   Use the arrows to navigate to STEP 1 - HEAT. Press ENTER. The number will start blinking. Use the arrows to set the desired value for the heat. Press ENTER. Navigate to STEP 1 – FORCE. Press ENTER. The number will start blinking. Use the arrows to set the desired value of force. Press ENTER. Navigate to STEP 1 – DISTANCE. Press ENTER. The number will start blinking. Use the arrows to set the desired value for the pulling distance. Press ENTER. If you are only using 1 stage, you do not need a delay. If you need more than one stage, set your delay and repeat the above for stage 2. Once you have successfully set all numbers of your desired settings, navigate to the SAVE (00). Press ENTER. The number will start blinking. Use the arrows to navigate to the program number where you want to save your new program. Once you reach the desired display press ENTER.   Modifying an Existing Protocol
  2. Getting Started with your PUL-1000 Micropipette Puller
    April 23, 2021
    The PUL-1000 is a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes. Here is a quick overview to get you started quickly.     Quick Start Setup Plug the power cord into the wall outlet. An LED light on the power supply illuminated when the unit is connected to power. Plug the power cord into the rear panel of the PUL-1000. Then, the power switch on the side panel illuminates. Press the Power button to turn on the display. To open the glass capillary clamps, place your thumb under the clamp and depress the release button with your index finger. This allows the carriages to move freely. Push the carriages together toward the center. Install the silicone glass capillary
  3. How to Run a Glass Softening Test on the PUL-1000 Puller
    April 22, 2021
    The PUL-1000 is a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes. We will show you how to run a glass softening test.       You should run the Softening Test when: You change the filament Lot numbers or capillary types change You create or modify a program The ambient environment changes Press the STOP key to quit any running program. The following window displays. Mount a glass capillary on the carriage. Press the START key to run the Glass Capillary Softening Test. The heating power increases gradually. The heating stops when the glass begins to move. Record the heating power. This is the baseline heating value for the glass type tested. It is a good starting point for the first stage of your program.   If you have any questions, just give us a call.   Buy Microelectrode Puller  
  4. Resolving Common Issues with the PUL-1000 Puller
    April 22, 2021
    The PUL-1000 is a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes. Here are the resolutions for some common issues with the puller.     Puller Stops If the puller stops in the middle of a pull and the unit beeps, one of two issues may be the culprit. The unit may be over heating. Allow the unit time to cool down before attempting to pull more glass. If a program fails in the middle of a pull, you may have exceeded the parameters of the unit. For example, when you add up the distance of travel for all the stages, you may have exceeded the maximum range of travel. Check your program. You may need to alter the heating or travel parameters to conform with the unit’s maximums.   Glass Doesn’t Pull Well If the glass does not pull well, look at one of these possible causes. The parameter may not be set properly for the properties of the new glass. Run the Glass Softening Test to establish a new baseline heating parameter. The filament may be wearing out or may have broken. Install a new filament.   Cannot Select a Program If you cannot select a program, the puller may be in Edit mode. It will not execute a program until you exit the Edit mode. Press the Exit key to exit the program editing mode without saving the program. Press Start to run your program.   Unit Beeps If the unit beeps and the program fails to start, the carriages may be too far apart. Remove the glass, slide the carriages together and reposition the glass.   If you have any questions, just give us a call.  
  5. How to Load Capillary Glass in a PUL-1000 Micropipette Puller
    April 22, 2021
    The PUL-1000 is a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes. Here we will see how to load a piece of glass into the carriage.     The glass capillary is held by clamps mounted on two movable carriages. Both carriages synchronously slide as a program is executed. Manually slide the carriages together when you are loading the glass. To open the clamps, place your thumb under the clamp and depress the release button with your index finger. Slide the glass capillary into the groove from one side. Slide it through the center of heating filament onto the other
  6. Get Familiar with Your New PUL-1000 Micropipette Puller
    April 09, 2021
    Let’s look at the PUL-1000, a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes. Here we will examine the design of the unit.         Tempered Glass Lid For safety, the lid should be closed whenever you are pulling glass or moving the unit. Capillary Glass Clamp Pads These two silicone clamp pads securely hold the glass when you are pulling pipettes. Check the glass OD and install appropriate pads accordingly. Heating Filament The PUL-1000 comes with a circular platinum/iridium box filament, which may be easily replaced w
  7. Why Buy a PUL-1000 Research Puller for Making Micropipettes?
    February 25, 2021
    PUL-1000 is a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes used in intracellular recording, patch clamp, microperfusion and microinjection. The PUL-1000 is capable of producing a vast array of pipette shapes, such as a long graduated taper, a short graduated taper and a short taper bee stingers. Here's a quick introduction the PUL-1000 research puller. The PUL-1000 puller was designed with tight mechanical specifications and precision electronics for complete control of the pulling process and accurate reproducibility. It offers programmable sequences of up to four steps with heating, force, movement and cooling time. A glass capillary is heated by a platinum/iridium filament and pulled by a controlled force. The PUL-1000 is capable of producing a vast array of pipette shapes, such as a long graduated taper, a short graduated taper and a short taper bee stingers. Programming The settings for a 4-stage pull may be stored in memory. Up to 95 programs can be permanently stored in memory. The instrument contains 15 factory installed programs. Choose from the factory installed programs for a quick start or create your own for precise, custom results.  Construction Tempered Glass Cover The cover of the pulling chamber is made with tempered glass to minimize the effects of humidity and air currents on the reproducibility of pulled pipettes.  Switching Power Supply
  8. Five Factors That Affect the Pulling of Glass Micropipettes
    February 22, 2021
    Pulling micropipettes or microelectrodes is a science and an art, requiring some finesse. Here we discuss the five major factors which can affect the shape of a pulled glass micropipette or microelectrode. The PUL-1000 is a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes. Here we will look at factors that affect the pulling of glass.  As the temperature of glass increases, the glass transitions gradually from a hard and relatively brittle solid state into a soft and viscous state. To form glass into certain shapes using a puller, heat is applied through a filament. Many factors affect the heat transferred from a filament to a glass capillary. The filament holders heat up when current passes through the filament, which
  9. Using WPI's PUL-1000 Micropipette Puller
    March 06, 2015
    World Precision Instruments' PUL-1000 is a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes used in intracellular recording, microperfusion and microinjection. It offers programmable sequences of up to four steps with complete control over the heating, force, movement and cooling time. This allows graduated cycles for a variety of applications. PUL-1000 can produce pipettes with tip diameters from less than 0.1µm to 10+ µm.  
  10. Microforges, Pullers, Bevelers Application Guide
    May 01, 2013
    Order code Description DMF1000 Description: Complete Microforge System; includes Programmable Digital Controller and Microscope
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