Microinjection Videos

Let’s look at the PUL-1000, a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes. Here we will examine the design of the unit.         Tempered Glass Lid For safety, the lid should be closed whenever you are pulling glass or moving the unit. Capillary Glass Clamp Pads These two silicone clamp pads securely hold the glass when you are pulling pipettes. Check the glass OD and install appropriate pads accordingly. Heating Filament The PUL-1000 comes with a circular platinum/iridium box filament, which may be easily replaced w

NanoFil™ is a unique low volume syringe developed for improved microinjection in mice and other small animals. Using a UMP3 UltraMicroPump combined with the syringe allows you to make repeatable injections in the nanoliter range. Here we'll show you how to install a NanoFil Syringe on the UMP3 UltraMicroPump.     NanoFil syringes are 10 µL and 100 µL syringes which can be installed on a UMP3 UltraMicroPump to give you repeatable injections in the nanoliter range. We will use the collar of the NanoFil syringe and the plunger as the two points to anchor the syringe to the pump.

NanoFil™ is a unique low volume syringe developed for improved microinjection in mice and other small animals. RPE and IO kits are specially designed for “hands-free” and oil-free sub-microliter injection. Originally developed for retinal pigment epithelium and intraocular injection, they can also be used for other applications.     The NanoFil syringe system utilizes a seal system that is based on a 0.46 mm shank diameter. Any needle or tubing with an outside diameter of 0.46 mm should fit the NanoFil’s seal system. Here is how to install your needle in a way that prevents damage to the gasket and keeps your needles straight.

NanoFil™ is a unique low volume syringe developed for improved microinjection in mice and other small animals. Here we'll show you how to install your needle in a way that prevents damage to the gasket and keeps your needles straight.     The NanoFil syringe system utilizes a seal system that is based on a 0.46 mm shank diameter. Any needle or tubing with an outside diameter of 0.46 mm should fit the NanoFil’s seal system. Here is how to install your needle in a way that prevents damage to the gasket and keeps your needles straight. PRO TIP: Always use surgical gloves to prevent the oils from your skin from affecting the

NanoFil™ is a unique low volume syringe developed for improved microinjection in mice and other small animals. During our field trials, the 35-gauge needle was scientists’ most popular and preferred NanoFil tip. The combination of its strength, length, durability and clogging resistance creates a solid balance. The 33-gauge tip is similar to Hamilton's 7762 and 7803 series removable needles in both tip length and outer diameter. However, our beveled tip version is shorter, more durable, and penetrates better due to the special tri-surface grinding technique. 33-gauge tips used to be the smallest size sold, but our 35-gauge tip is much better for injections involving small ani

NanoFil™ is a unique low volume syringe developed for improved microinjection in mice and other small animals. NanoFil needles are specially designed with a small outside diameter and spear point for shallow penetration and to cause the least amount of tissue damage.    Specially designed tips as small as 36 gauge (110 micron OD) are offered in both blunt and beveled styles. These tips will cause less trauma to the tissue than any other form of micro syringe currently in use. NanoFil can be used with many forms of small tubing and tips coupled with the syringe barrel. The blunt tip is used for injection into soft tissue a

NanoFil™ is a unique low volume syringe developed for improved microinjection in mice and other small animals. Here we show you several ways in which the NanoFil can be used for making injections. You can install the syringe on WPI’s UMP3 UltraMicroPump, using SilFlex tubing and a holder, so you can accurately inject by hand. You can install it in a stereotaxic frame. You can also directly inject by hand. NanoFil can be used in several different configurations: Install it on WPI’s UMP3 UltraMicroPump, which allows nanoliter resolution and reproducibility. For neural system injection, you can m

NanoFil™ is a unique low volume syringe developed for improved microinjection in mice and other small animals. It comes in both 10 and 100 microliter volumes and can be paired with a UMP3 UltraMicroPump for making accurate microliter injections. NanoFil stainless steel needles range in size from 33 to 36 gauge. NanoFil™ is a unique low volume syringe developed for improved microinjection in mice and other small animals. It makes quantitative nanoliter injection much easier and more accurate than any other method. NANOFIL is a 10microliter syringe, and NANOFIL-100 is a 100 µL syringe. The large volu

PUL-1000 is a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes used in intracellular recording, patch clamp, microperfusion and microinjection. The PUL-1000 is capable of producing a vast array of pipette shapes, such as a long graduated taper, a short graduated taper and a short taper bee stingers. Here's a quick introduction the PUL-1000 research puller. The PUL-1000 puller was designed with tight mechanical specifications and precision electronics for complete control of the pulling process and accurate reproducibility. It offers programmable sequences of up to four steps with heating, force, movement and cooling time. A glass capillary is heated by a platinum/iridium filament and pulled by a controlled force. The PUL-1000 is capable of producing a vast array of pipette shapes, such as a long graduated taper, a short graduated taper and a short taper bee stingers. Programming The settings for a 4-stage pull may be stored in memory. Up to 95 programs can be permanently stored in memory. The instrument contains 15 factory installed programs. Choose from the factory installed programs for a quick start or create your own for precise, custom results.  Construction Tempered Glass Cover The cover of the pulling chamber is made with tempered glass to minimize the effects of humidity and air currents on the reproducibility of pulled pipettes.  Switching Power Supply

Pulling micropipettes or microelectrodes is a science and an art, requiring some finesse. Here we discuss the five major factors which can affect the shape of a pulled glass micropipette or microelectrode. The PUL-1000 is a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes. Here we will look at factors that affect the pulling of glass.  As the temperature of glass increases, the glass transitions gradually from a hard and relatively brittle solid state into a soft and viscous state. To form glass into certain shapes using a puller, heat is applied through a filament. Many factors affect the heat transferred from a filament to a glass capillary. The filament holders heat up when current passes through the filament, which