Microinjection Videos

Do you want to take the next step in your CRISPR-CAS9 projects, making the injection of biomolecules in your cell lines more efficient? You can now increase the viability of injected embryos with WPI’s MICRO-ePORE,

The PUL-1000 is a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes. Here is how to run a program that will pull two identical microelectrodes.   Loading a Program   We will use the keypad to navigate through the display. Use the arrows to navigate to LOAD (00). The top and bottom arrows on the keypad navigate numbers in increments of 1. The left and right arrows on the keypad navigate numbers in increments of 10. Press ENTER. The number will start blinking. Use the arrows to navigate to the program number you would like load. Once you reach the desired program, press ENTER to run the program and pull your glass.   Creating a New Program   Use the arrows to navigate to STEP 1 - HEAT. Press ENTER. The number will start blinking. Use the arrows to set the desired value for the heat. Press ENTER. Navigate to STEP 1 – FORCE. Press ENTER. The number will start blinking. Use the arrows to set the desired value of force. Press ENTER. Navigate to STEP 1 – DISTANCE. Press ENTER. The number will start blinking. Use the arrows to set the desired value for the pulling distance. Press ENTER. If you are only using 1 stage, you do not need a delay. If you need more than one stage, set your delay and repeat the above for stage 2. Once you have successfully set all numbers of your desired settings, navigate to the SAVE (00). Press ENTER. The number will start blinking. Use the arrows to navigate to the program number where you want to save your new program. Once you reach the desired display press ENTER.   Modifying an Existing Protocol

The PUL-1000 is a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes. Here is a quick overview to get you started quickly.     Quick Start Setup Plug the power cord into the wall outlet. An LED light on the power supply illuminated when the unit is connected to power. Plug the power cord into the rear panel of the PUL-1000. Then, the power switch on the side panel illuminates. Press the Power button to turn on the display. To open the glass capillary clamps, place your thumb under the clamp and depress the release button with your index finger. This allows the carriages to move freely. Push the carriages together toward the center. Install the silicone glass capillary

The PUL-1000 is a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes. We will show you how to run a glass softening test.       You should run the Softening Test when: You change the filament Lot numbers or capillary types change You create or modify a program The ambient environment changes Press the STOP key to quit any running program. The following window displays. Mount a glass capillary on the carriage. Press the START key to run the Glass Capillary Softening Test. The heating power increases gradually. The heating stops when the glass begins to move. Record the heating power. This is the baseline heating value for the glass type tested. It is a good starting point for the first stage of your program.   If you have any questions, just give us a call.   Buy Microelectrode Puller  

The PUL-1000 is a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes. Here are the resolutions for some common issues with the puller.     Puller Stops If the puller stops in the middle of a pull and the unit beeps, one of two issues may be the culprit. The unit may be over heating. Allow the unit time to cool down before attempting to pull more glass. If a program fails in the middle of a pull, you may have exceeded the parameters of the unit. For example, when you add up the distance of travel for all the stages, you may have exceeded the maximum range of travel. Check your program. You may need to alter the heating or travel parameters to conform with the unit’s maximums.   Glass Doesn’t Pull Well If the glass does not pull well, look at one of these possible causes. The parameter may not be set properly for the properties of the new glass. Run the Glass Softening Test to establish a new baseline heating parameter. The filament may be wearing out or may have broken. Install a new filament.   Cannot Select a Program If you cannot select a program, the puller may be in Edit mode. It will not execute a program until you exit the Edit mode. Press the Exit key to exit the program editing mode without saving the program. Press Start to run your program.   Unit Beeps If the unit beeps and the program fails to start, the carriages may be too far apart. Remove the glass, slide the carriages together and reposition the glass.   If you have any questions, just give us a call.  

The PUL-1000 is a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes. Here we will see how to load a piece of glass into the carriage.     The glass capillary is held by clamps mounted on two movable carriages. Both carriages synchronously slide as a program is executed. Manually slide the carriages together when you are loading the glass. To open the clamps, place your thumb under the clamp and depress the release button with your index finger. Slide the glass capillary into the groove from one side. Slide it through the center of heating filament onto the other

Let’s look at the PUL-1000, a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes. Here we will examine the design of the unit.         Tempered Glass Lid For safety, the lid should be closed whenever you are pulling glass or moving the unit. Capillary Glass Clamp Pads These two silicone clamp pads securely hold the glass when you are pulling pipettes. Check the glass OD and install appropriate pads accordingly. Heating Filament The PUL-1000 comes with a circular platinum/iridium box filament, which may be easily replaced w

NanoFil™ is a unique low volume syringe developed for improved microinjection in mice and other small animals. Using a UMP3 UltraMicroPump combined with the syringe allows you to make repeatable injections in the nanoliter range. Here we'll show you how to install a NanoFil Syringe on the UMP3 UltraMicroPump.     NanoFil syringes are 10 µL and 100 µL syringes which can be installed on a UMP3 UltraMicroPump to give you repeatable injections in the nanoliter range. We will use the collar of the NanoFil syringe and the plunger as the two points to anchor the syringe to the pump.

NanoFil™ is a unique low volume syringe developed for improved microinjection in mice and other small animals. RPE and IO kits are specially designed for “hands-free” and oil-free sub-microliter injection. Originally developed for retinal pigment epithelium and intraocular injection, they can also be used for other applications.     The NanoFil syringe system utilizes a seal system that is based on a 0.46 mm shank diameter. Any needle or tubing with an outside diameter of 0.46 mm should fit the NanoFil’s seal system. Here is how to install your needle in a way that prevents damage to the gasket and keeps your needles straight.

NanoFil™ is a unique low volume syringe developed for improved microinjection in mice and other small animals. Here we'll show you how to install your needle in a way that prevents damage to the gasket and keeps your needles straight.     The NanoFil syringe system utilizes a seal system that is based on a 0.46 mm shank diameter. Any needle or tubing with an outside diameter of 0.46 mm should fit the NanoFil’s seal system. Here is how to install your needle in a way that prevents damage to the gasket and keeps your needles straight. PRO TIP: Always use surgical gloves to prevent the oils from your skin from affecting the