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Lab Supplies

  1. Get Familiar with Your New PUL-1000 Micropipette Puller
    April 09, 2021
    Let’s look at the PUL-1000, a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes. Here we will examine the design of the unit.         Tempered Glass Lid For safety, the lid should be closed whenever you are pulling glass or moving the unit. Capillary Glass Clamp Pads These two silicone clamp pads securely hold the glass when you are pulling pipettes. Check the glass OD and install appropriate pads accordingly. Heating Filament The PUL-1000 comes with a circular platinum/iridium box filament, which may be easily replaced w
  2. How to Read a Vernier Scale
    April 08, 2021
    Vernier scales can be used on microscopes, stereotaxic frames and micromanipulators. The vernier scale was invented by French mathematician Pierre Vernier in 1631 as an upgrade on Pedro Nunes' measurement system for precision astrolobes. With a main scale and a sliding secondary scale, a vernier is used for making precise measurements.    How a Vernier Scale Works The vernier scale is marked with divisions slightly smaller than the divisions of the main scale. For example, a vernier scale could have 11 markings for every 10 on the main scale. That's 10 divisions on the vernier scale for every 9 on the main scale. This means that the vernier divisions are each 90% of the main scale divisions. In this case, the 0-line and the 10-line on the vernier could pair up with marks on the main scale, but none of the other divisions on the vernier would match a line of the main scale. For example, the 0 and 10-lines of the vernier scale could pair up with the 0 and 9-lines on the main scale. If the 0-line pairs up with a mark, the first division of the vernier (1 mark) would be 10% short of reaching a mark of the main scale, the second division (2 mark) would miss a mark on the main scale by 20%, the third division (3 mark) would miss a mark on the main scale by 30%, etc.   How to Read a Linear Vernier Scale Follow these steps to read the vernier scale: Read the main scale. Look for the last whole increment visible before the 0 (zero) mark. Read the secondary scale (Vernier) measurement. This is the division tick mark that lines up best with a mark on the main scale. Add the two measurements together. The image at the right shows a linear scale. The 0 on the vernier scale lines up with the 4 on the main scale. Notice that the 10 on the vernier scale also lines up with a mark on the main scale (4.9). We ignore the second mark that lines up. So, the measurement shown is 4.00mm. The second
  3. How to Safely Use a Peristaltic Pump
    April 06, 2021
    When using the Peri-Star Pro, solutions pumped should never come into contact with the pump. Here we will give you some guidelines for operating your peristaltic pump in a way that is safe for both the laboratory personnel and for the machinery.   Some solvents can permanently damage a peristaltic pump. The following rules are recommended to avoid leakage when running your Peri-Star Pro peristaltic pump. Follow safety rules when handling aggressive solvents. Pull a fresh section of tubing into the tubing fixtures regularly. If the pump gets heavy use, do this weekly. A fresh section of tubing should be pulled through the pump rollers before the pump is left to run unattended. Change the tubing immediately, if it is visibly worn. Be sure the
  4. How to Choose Tubing for Your Peristaltic Pump
    April 06, 2021
    A peristaltic pump is a popular appliance in any laboratory. Here you will find some tips for choosing the correct tubing to use for your application with your Peri-Star Pro peristaltic pump.     Size (ID) of Tubing In a peristaltic pump, the flow rate is determined by both the size of the tubing and the rotor speed. WPI provides two sizes of silicone tubing: 1/16-inch ID tubing for low flow rate at 0.2-18mL/min. 1/4-inch ID tubing for high flow rate at 3.5-280mL/min.   Types of Tubing Silicone tubing has a long service life and good chemical compatibility for aqueous and many organic solvents. Fluoroelastomer tubing should be used when pumping halogenated solvents. Vinyl tubing is
  5. Five Reasons to choose the Peri-Star Pro Peristaltic Pump
    April 06, 2021
    A peristaltic pump is a type of positive displacement pump used for pumping a variety of fluids, particularly clean, sterile or aggressive fluids. Here are five reasons to choose the Peri-Star Pro peristaltic pump.   The fluid contacts only the interior of the flexible tube and thus avoids contamination from the pump or damage to the pump. This makes it easy to maintain and clean. The Peri-Star Pro peristaltic pump provides accurate and precise fluid delivery. It can run at flow rates from 0.1mL/min. to 280mL/min. with better than 0.5% accuracy. Peri-Star Pro has a backlit digital LCD display and a water-resistant membrane keypad. A unique planetary gear design with eight actively driven rollers (four rollers in the higher flow rate model), together
  6. How to Install NanoFil Syringe on the UMP3
    February 25, 2021
    NanoFil™ is a unique low volume syringe developed for improved microinjection in mice and other small animals. Using a UMP3 UltraMicroPump combined with the syringe allows you to make repeatable injections in the nanoliter range. Here we'll show you how to install a NanoFil Syringe on the UMP3 UltraMicroPump.     NanoFil syringes are 10 µL and 100 µL syringes which can be installed on a UMP3 UltraMicroPump to give you repeatable injections in the nanoliter range. We will use the collar of the NanoFil syringe and the plunger as the two points to anchor the syringe to the pump.
  7. How to Use NanoFil Syringe for RPE and IO Injections
    February 25, 2021
    NanoFil™ is a unique low volume syringe developed for improved microinjection in mice and other small animals. RPE and IO kits are specially designed for “hands-free” and oil-free sub-microliter injection. Originally developed for retinal pigment epithelium and intraocular injection, they can also be used for other applications.     The NanoFil syringe system utilizes a seal system that is based on a 0.46 mm shank diameter. Any needle or tubing with an outside diameter of 0.46 mm should fit the NanoFil’s seal system. Here is how to install your needle in a way that prevents damage to the gasket and keeps your needles straight.
  8. How to Install the NanoFil Syringe Needles
    February 25, 2021
    NanoFil™ is a unique low volume syringe developed for improved microinjection in mice and other small animals. Here we'll show you how to install your needle in a way that prevents damage to the gasket and keeps your needles straight.     The NanoFil syringe system utilizes a seal system that is based on a 0.46 mm shank diameter. Any needle or tubing with an outside diameter of 0.46 mm should fit the NanoFil’s seal system. Here is how to install your needle in a way that prevents damage to the gasket and keeps your needles straight. PRO TIP: Always use surgical gloves to prevent the oils from your skin from affecting the
  9. How to Choose the Correct NanoFil Tip Size for Microinjections
    February 25, 2021
    NanoFil™ is a unique low volume syringe developed for improved microinjection in mice and other small animals. During our field trials, the 35-gauge needle was scientists’ most popular and preferred NanoFil tip. The combination of its strength, length, durability and clogging resistance creates a solid balance. The 33-gauge tip is similar to Hamilton's 7762 and 7803 series removable needles in both tip length and outer diameter. However, our beveled tip version is shorter, more durable, and penetrates better due to the special tri-surface grinding technique. 33-gauge tips used to be the smallest size sold, but our 35-gauge tip is much better for injections involving small ani
  10. NanoFil Needles Cause Less Tissue Damage
    February 25, 2021
    NanoFil™ is a unique low volume syringe developed for improved microinjection in mice and other small animals. NanoFil needles are specially designed with a small outside diameter and spear point for shallow penetration and to cause the least amount of tissue damage.    Specially designed tips as small as 36 gauge (110 micron OD) are offered in both blunt and beveled styles. These tips will cause less trauma to the tissue than any other form of micro syringe currently in use. NanoFil can be used with many forms of small tubing and tips coupled with the syringe barrel. The blunt tip is used for injection into soft tissue a
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