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Stereotaxic Instruments

  1. How to Read a Vernier Scale
    April 08, 2021
    Vernier scales can be used on microscopes, stereotaxic frames and micromanipulators. The vernier scale was invented by French mathematician Pierre Vernier in 1631 as an upgrade on Pedro Nunes' measurement system for precision astrolobes. With a main scale and a sliding secondary scale, a vernier is used for making precise measurements.    How a Vernier Scale Works The vernier scale is marked with divisions slightly smaller than the divisions of the main scale. For example, a vernier scale could have 11 markings for every 10 on the main scale. That's 10 divisions on the vernier scale for every 9 on the main scale. This means that the vernier divisions are each 90% of the main scale divisions. In this case, the 0-line and the 10-line on the vernier could pair up with marks on the main scale, but none of the other divisions on the vernier would match a line of the main scale. For example, the 0 and 10-lines of the vernier scale could pair up with the 0 and 9-lines on the main scale. If the 0-line pairs up with a mark, the first division of the vernier (1 mark) would be 10% short of reaching a mark of the main scale, the second division (2 mark) would miss a mark on the main scale by 20%, the third division (3 mark) would miss a mark on the main scale by 30%, etc.   How to Read a Linear Vernier Scale Follow these steps to read the vernier scale: Read the main scale. Look for the last whole increment visible before the 0 (zero) mark. Read the secondary scale (Vernier) measurement. This is the division tick mark that lines up best with a mark on the main scale. Add the two measurements together. The image at the right shows a linear scale. The 0 on the vernier scale lines up with the 4 on the main scale. Notice that the 10 on the vernier scale also lines up with a mark on the main scale (4.9). We ignore the second mark that lines up. So, the measurement shown is 4.00mm. The second
  2. Rodent Facemask Kits for Isoflurane Anesthesia
    July 14, 2014
    Gas anesthesia with Isoflurane is quickly becoming the standard method of general anesthesia for rats and mice in biomedical research. The advantages of using gas anesthesia for in-vivo examinations include: Fewer complications than injectable agents Allows for longer exam duration Easier administration and control Readily incorporated into existing procedures Level plane of anesthesia Minimal animal handling/Less stress No controlled substances required Quick recovery time Economical and versatile These are designed to tightly and flexibly fit on various size rodents. The optically clear masks are designed to provide access to the
  3. Using a Microscope with a Stereotaxic Frame
    April 30, 2013
    You can use the PZMIV stereo microscope with a stereotaxic frame as shown in the image below. This setup shows a PZMIV-BS. The U-frame Base Plate (502045) is shown, but most stereotaxic frames can be used in this way. Choose a stereo microscope objective that allows you plenty of room to work. For example, the 0.5X objective has 187mm working distance, or the 0.32X objective has 296mm working distance. You could also add a Z-LITE-Z186 illuminator. If necessary, use a 5 to 10 lb.counter weight on the boom stand base to prevent the microscope from tipping.
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