Reliable Pneumatic PicoPump

$2,541.00
Order code
SYS-PV820

Pneumatic microinjector with vacuum pressure

  • Inject into a single cell with picoliter volumes
  • Regulated Hold and Ejection Pressure
  • Carefully regulated air pressures for securing cells and injecting them with fluid
  • Pressure Input: 0-150 psi
  • Pressure Output: 0.3-90 psi

See the current Spec Sheet and the Microinjection Spec Sheet.

Benefits

  • Hold pressure prevents backfilling of the pipette by capillary action
  • PV830 includes vacuum pressure for filling pipettes from the tip or securing floating cells during injection

Applications

  • Intracellular injection in the picoliter to nanoliter range

Although syringe and piston techniques are popular for microinjection in the nanoliter range, microinjection of sub-nanoliter volumes through a very small opening in a glass micropipette is often better accomplished with a controlled burst of gas pressure. Designed to simplify intracellular injection and a variety of other micro­in­jec­tion tasks, WPI’s PicoPumps use precisely regulated pres­sures for se­cur­ing cells and injecting them with fluid. Injected volumes range from picoliters to nanoliters. Separate ports supply positive and negative pressure—pos­i­tive pressure for high-pressure ejection, and suction for supporting the cell or for filling the pipette from the tip. In the time between injections, a secondary default “hold” pressure is applied to the pipette to prevent fluid up­take through capillary action or diffusion. The pressure pulses are typically controlled by a precision timer. Timing, ejection pressure, hold­ing pressure and suction are adjusted inde­pen­dent­ly by control knobs and monitored with in­di­ca­tor gauges on the front panel. Injection pressure is controlled by a 20-turn regulator on the front panel. The built-in timing function controls the amount of time that the injection pressure is applied with millisecond resolution. Time intervals can range from 10 s down to 10 ms or less. The pressure burst can also be controlled manually or triggered by an external source.

The PV830 model provides separate regulation and pressure gauges for the eject and hold pressures. In addition to the features of the PV820, the PV830 model also includes a vacuum pressure regulator and vacuum gauge.

PicoNozzle Kit 5430-ALL (included) allows micropipettes to be securely mount­ed in micro-positioners for stable axial air delivery.

5430-ALL

The optional 5430-XX PicoNozzle Kit has a Luer-fitted pipette holder for quick and easy changes. Choose your kit based on the pipette OD (XX indicates OD: 1.0, 1.2, 1.5 or 2.0 mm). The red handle has a larger diameter so that it fits easily into most manipulators. This kit contains:

(1) MPH6S microelectrode holder

(1) Handle for the MPH6S (4" hollow tube with male Luer fitting at both ends-handle diameter is 6.25 x 100 mm.)

(1) 5’ tubing (0.060" ID, 0.120" OD, male locking Luer fitting on one end and a female locking Luer fitting at the other end, rated for 200 PSI and 86 durometer shore A)

Hold Pressure Prevents Backfilling

Like the PV830, the PV820 was designed to simplify intracellular injection. The hold pressure prevents backfilling of the pipette by capillary action. Simple to use. Reliable every time.

Each PicoPump is supplied with a 5430-ALL kit that includes two PicoNozzles and tubing to connect the holders to the pressure and vacuum ports.

Vacuum Pressure for Additional Applications

Eject pressure supplies a high-pressure pulse for injecting fluid. Hold pressure, which is not sufficient to cause fluid ejection, is used to prevent back filling of the pipette by capillary action or diffusion when the solenoid is inactive. Vacuum can be applied through the ejection port, but vacuum in the PV820 must be externally regulated. To fill pi­pettes from the tip or through a secondary port to secure a floating cell during microinjection, vacuum may be switched from regulated vacuum to at­mo­sphere by using the switch on the front panel.

PV820 Instruction Manual
Syringe Volume Calculation Spreadsheet- Use this .XLS spreadsheet to calculate the volume of your syringe when you are using an UMP3, DMP, MMP or PV820/PV830. 

PRESSURE
Pressure Input 0-150 PSI
Pressure Output 0.3-90 PSI
Pressure Burst Timer (10-turn dial) 10 ms to 10 s in Timed Mode
Regulator Accuracy 0.1% (20-turn dial)
Regulator Repeatability 0.05 PSI (both hold and eject pressures)
Gauge Accuracy 3% at full scale (both hold and eject pressures)
Input Connector Quick Connect (1/4" OD tubing)
Output Connector Barbed (1/16" ID tubing)
VACUUM
Vacuum Input 0-30.0" Hg
Vacuum Output Unregulated
Lowest Regulated Vacuum Unregulated
Regulator Accuracy Unregulated
Regulator Repeatability Unregulated
Gauge Accuracy None
Input Connector Quick Connect (1/4" OD tubing)
Output Connector Barbed (1/16" ID tubing)
Control Manual
Vent Atmosphere
CONNECTIONS INCLUDED
Input Kit 10' nylon tubing (0.25" OD, 1000 PSI), one 1/4" female NPT adapter
Output Kit Two PicoNozzle
PHYSICAL SPECS
Power 95-135V or 220-240V, 50/60Hz
Dimensions 17 x 3.5 x 9.5" (43 x 9 x 24cm)
Shipping Weight 11 lb. (5 kg)

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Farah, C. A., & Sossin, W. S. (2011). Live-imaging of PKC Translocation in Sf9 Cells and in Aplysia Sensory Neurons. Journal of Visualized Experiments, (50), e2516–e2516. https://doi.org/10.3791/2516

Sundvik, M., Kudo, H., Toivonen, P., Rozov, S., Chen, Y.-C., & Panula, P. (2011). The histaminergic system regulates wakefulness and orexin/hypocretin neuron development via histamine receptor H1 in zebrafish. The FASEB Journal, 25(12), 4338–4347. https://doi.org/10.1096/fj.11-188268

Cianciolo Cosentino, C., Roman, B. L., Drummond, I. A., & Hukriede, N. A. (2010). Intravenous microinjections of zebrafish larvae to study acute kidney injury. Journal of Visualized Experiments : JoVE, (42). https://doi.org/10.3791/2079

Kague, E., Weber, C., & Fisher, S. (2010). Mosaic Zebrafish Transgenesis for Evaluating Enhancer Sequences. Journal of Visualized Experiments, (41), e1722–e1722. https://doi.org/10.3791/1722

Lee, G.-H., Ahn, T., Kim, D.-S., Park, S. J., Lee, Y. C., Yoo, W. H., … Chae, H.-J. (2010). Bax inhibitor 1 increases cell adhesion through actin polymerization: involvement of calcium and actin binding. Molecular and Cellular Biology, 30(7), 1800–1813. https://doi.org/10.1128/MCB.01357-09

Chen, H.-L., Yuh, C.-H., & Wu, K. K. (2010). Nestin Is Essential for Zebrafish Brain and Eye Development through Control of Progenitor Cell Apoptosis. PLoS ONE, 5(2), e9318. https://doi.org/10.1371/journal.pone.0009318

Russek-Blum, N., Nabel-Rosen, H., & Levkowitz, G. (2010). Two-photon-based photoactivation in live zebrafish embryos. Journal of Visualized Experiments : JoVE, (46). https://doi.org/10.3791/1902

Deiters, A., Garner, R. A., Lusic, H., Govan, J. M., Dush, M., Nascone-Yoder, N. M., & Yoder, J. A. (2010). Photocaged morpholino oligomers for the light-regulation of gene function in zebrafish and Xenopus embryos. Journal of the American Chemical Society, 132(44), 15644–15650. https://doi.org/10.1021/ja1053863

Bill, B. R., Petzold, A. M., Clark, K. J., Schimmenti, L. A., & Ekker, S. C. (2009). A Primer for Morpholino Use in Zebrafish. Zebrafish, 6(1), 69–77. https://doi.org/10.1089/zeb.2008.0555

Yuan, S., & Sun, Z. (2009). Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos. Journal of Visualized Experiments, (27), e1113–e1113. https://doi.org/10.3791/1113

Faustino, R. S., Cheung, P., Richard, M. N., Dibrov, E., Kneesch, A. L., Deniset, J. F., … Pierce, G. N. (2008). Ceramide regulation of nuclear protein import. Journal of Lipid Research, 49(3), 654–662. https://doi.org/10.1194/jlr.M700464-JLR200

Sim, J. A., Broomhead, H. E., & North, R. A. (2008). Ectodomain lysines and suramin block of P2X1 receptors. The Journal of Biological Chemistry, 283(44), 29841–29846. https://doi.org/10.1074/jbc.M802523200

Faustino, R. S., Stronger, L. N. W., Richard, M. N., Czubryt, M. P., Ford, D. A., Prociuk, M. A., … Pierce, G. N. (2007). RanGAP-Mediated Nuclear Protein Import in Vascular Smooth Muscle Cells Is Augmented by Lysophosphatidylcholine. Molecular Pharmacology, 71(2).

Reig, R., Sanchez-Vives, M. V., Evarts, E., Hobson, J., McCarley, R., Steriade, M., … Fetz, E. (2007). Synaptic Transmission and Plasticity in an Active Cortical Network. PLoS ONE, 2(8), e670. https://doi.org/10.1371/journal.pone.0000670

Dean, D. A. (2006). Gene delivery by direct injection (microinjection) using a controlled-flow system. CSH Protocols, 2006(7), pdb.prot4654. https://doi.org/10.1101/pdb.prot4654

Method of identifying chemical agents which stimulate odorant receptors of sensory neurons. (2004).

Integrated biochip with continuous sampling and processing (csp) system. (2004).

Hanstein, S. M., & Felle, H. H. (2004). Nanoinfusion: an integrating tool to study elicitor perception and signal transduction in intact leaves. New Phytologist, 161(2), 595–606. https://doi.org/10.1111/j.1469-8137.2004.00971.x

Kindig, C. A., Howlett, R. A., & Hogan, M. C. (2003). Effect of extracellular Po 2 on the fall in intracellular Po 2 in contracting single myocytes. Journal of Applied Physiology, 94(5), 1964–1970. https://doi.org/10.1152/japplphysiol.00893.2002

Dormann, D., Abe, T., Weijer, C. J., & Williams, J. (2001). Inducible nuclear translocation of a STAT protein in Dictyostelium prespore cells: implications for morphogenesis and cell-type regulation. Development (Cambridge, England), 128(7), 1081–1088. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/11245573

Gutiérrez, A. A., Arias, J. M., García, L., Mas-Oliva, J., & Guerrero-Hernández, A. (1999). Activation of a Ca 2+ -permeable cation channel by two different inducers of apoptosis in a human prostatic cancer cell line. The Journal of Physiology, 517(1), 95–107. https://doi.org/10.1111/j.1469-7793.1999.0095z.x

Nishizaki, T., & Mori, M. (1998). Diverse Signal Transduction Pathways Mediated by Endogenous P2 Receptors in Cultured Rat Cerebral Cortical Neurons. Journal of Neurophysiology, 79(5).

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More Choices:
  1. PicoPump Foot Switch
    PicoPump Foot Switch
    3260
    $247.00
  2. PicoNozzle Kit v2
    PicoNozzle Kit v2
    5430-ALL
    $184.00
  3. Rack Mount Kit, 3 1/2-in. high
    Rack Mount Kit, 3 1/2-in. high
    2932
    $127.00
  4. Rack Mount Kit, 5 1/4-in. high
    Rack Mount Kit, 5 1/4-in. high
    2933
    $127.00
  5. Replacement Pipette for 5430-ALL
    Replacement Pipette for 5430-ALL
    75125-6

    Please Contact WPI for Pricing

  6. PicoNozzle Gasket, 1.65mm, Red, Pkg of 10
    PicoNozzle Gasket, 1.65mm, Red, Pkg of 10
    Multiple SKUs
    $64.00
  7. PicoNozzle Kit v1
    PicoNozzle Kit v1
    Multiple SKUs
    $184.00
  8. Microelectrode Holder (MPH6S)
    Microelectrode Holder (MPH6S)
    Multiple SKUs
    $71.00
  9. Microelectrode Holder (MPH6R)
    Microelectrode Holder (MPH6R)
    Multiple SKUs
    $71.00

Microinjection System

The PV820 is our popular pump for microinjection. A basic system shown below includes:

  • PZMIII-MI  microscope with lighted base
  • PV820 PicoPump
  • M3301 Micromanipulator
  • 5430-ALL PicoNozzle Kit
  • M10 magnetic stand
  • 5052 steel base plate

Also shown in the image is the PUL-1000 micropipette puller, surgical instruments, pre-pulled MicroTips and some capillary glass.

This system is ideal for use with Zebrafish, C. Elegans, Drosophila and Xenopus. Our customizable microinjection system is also suitable for microinjection applications, including CRISPR/Cas9.  

The system is completely customizable. To see some of the options, take a look at the Microinjection Toolbox article on the blog.

Microinjection System includes the microscope with lighted base, a pump, a micromanipulator, an injector and accessories.

If you have any questions about our Microinjection Systems, please give us a call at (866) 606-1974 (Toll free in the USA) or email us at wpi@wpiinc.com.

 

 

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