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TEER

  1. Choosing an EndOhm Chamber Compatible with your Cell Culture Inserts
    February 21, 2019
    WPI's EndOhm chambers are used with WPI's EVOM2 meter to measure TEER (transepithelial electrical resistance) in cell culture inserts. Three chamber sizes are available for 6-well plates, 12-well plates and 24-well plates. Here Subhra demonstrates how to choose an EndOhm chamber to match the cell culture inserts you are using.
  2. Prevent Damage to Snapwell Inserts by Using STX3 Electrode
    February 21, 2019
    The WPI EVOM2 meter is used to measure TEER (transepithelial electrical resistance) in cell culture inserts. The STX2 electrodes with a static length may cause damage to Snapwell inserts. Here Subhra demonstrates how to use the adjustable STX3 electrode to prevent damage to your cell culture inserts.
  3. How To Select Electrodes For Making TEER Measurements
    November 28, 2018
    Selection of Electrodes for TEER Measurements Transepithelial electrical resistance (TEER), also referred as the transepithelial resistance (TER) is used to monitor cellular health. TEER is comprised of measurements of the transcellular pathway (i.e., resistance due to an individual cell) and paracellular pathway (i.e., resistance due to the formation of the cellular junctions). TEER is commonly used to monitor cellular confluence. TEER values can indicate changes in the cellular monolayer permeability, showing the monolayer barrier function of cells such as, endothelial (brain microvessel) and epithelial (alveolar, kidney, and intestinal) cells. High TEER values generally reflect tighter cellular monolayers or cellular junctions (Lewis 1996, Matter and Balda 2003, Denker and Sabath 2011). A few major benefits of WPI TEER measurement systems are described below. The TEER values (electrophysiological analysis) can be combined with other analysis methods to further understand
  4. Monitor the Growth of Epithelial Cells in Vitro Manually
    November 20, 2015
    For small batches of cell culture Are you ready to perform experiments involving a cultured cell monolayer? If your monolayer has not reached confluence, you may have holes or gaps in the monolayer that would prevent collection of valid experimental data. When confluence is reached, the electrical resistance across the monolayer peaks and then plateaus. The EVOM2 meter was designed specifically for this application. The EVOM2 is the ideal meter for use when you are making manual TEER measurements on small batches of cell cultures and do not require a high throughput system. EVOM2 - Perfect for TEER The EVOM2
  5. Setting Up Your Automated TEER Measurement Robot
    April 14, 2015
    Now that your REMS system has arrived, are you trying to figure out how to set it up? Check out this 5-minute video where we show you how, step-by-step. More Info
  6. FAQs about TEER Measurement
    February 02, 2015
    Here are some frequently asked questions (FAQ) about TEER measurement using an EVOM2. Are the electrical resistance and transepithelial electrical resistance (TEER) the same thing? What is an EndOhm chamber? How is an EVOM2 used for measuring confluence? Why use an EndOhm instead of a STX? How do I clean my electrode? What about electrode preconditioning? Can you give me a simple data acquisition system for TEER? What are the TEER measurement challenges that I may encounter? Can you suggest some experimental
  7. Recording TEER Measurements from an EVOM2
    November 11, 2014
    The EVOM2 is the classic, hand-held instrument for making Trans Epithelial Electrical Resistance (TEER) measurements. The REMS system adds a robot and data recording for automated TEER measurements. There may be times when you don't have a robot, but would still like to have the data recording capabilities of the REMS system. With a little ingenuity, you can do just that. Here, we will show you how you can add data recording to your standard EVOM2. Open Labscribe3 on your computer. Let the program identify the data recorder. In our example, we will use a Lab-Trax-4. Choose the default settings. Setup the preferences for one channel. If you like, you may add five points of smoothing.  In the marks section of the main window, select A1 (channel one). This records a data point directly into the marks records window. Alternatively, the smoothed graph C1 may be selected. In the top graph, right click and choose Units>Simple to calibrate the graph to ohms. Enter these values for the EVOM2 conversion: 2 point calibration Apply units to new data Value at Cursor 1: 0.0000 => 0.0000 Value at Cursor 2: 1.0000 => 1000 Unit Name = Ohms To connect the EVOM2 to the data recorder, connect a BNC cable from the Output BNC on the front of the EVOM2 to the Channel 1 input BNC on your data recorder. In the Marks window, enter A1 or Control. Move the electrode to the control well, and allow it to stabilize. Then, press Enter. Otherwise, it is difficult to hold the electrodes still while typing. For each additional well, enter in the number A2…, A3 into the Marks field. Then, move the electrode to that well and press the enter key after the reading stabilizes. Stop the recording and save the file. Open up the marks editor in Labscribe (View>Marks>Marks). The dialog box shows the time mark data and resistance values.   Press the Export button and choose “All Marks.” Give your export file a name. The export files is a text file that may be import into Microsoft Excel or another spreadsheet application.
  8. REMS High Throughput TEER Robot Gets Software Upgrade
    May 30, 2014
    What is REMS? The REMS AutoSampler automates measurements of electrical resistance of transepithelial, transendothelial or Caco-2 cell membranes being grown to confluence on microporous filters of high throughput screening (HTS) 24- and 96-well microplates. It is a PC-controlled, tissue resistance measurement system that offers reproducibility, accuracy and flexibility for this kind of measurement. The ability of the REMS AutoSampler to precisely locate the electrode results in highly reproducible TEER measurements. TEER measurements are stored in the computer as the electrode moves from one well to the next. The Windows-based software provides user-friendly features to acquire, display and store the tissue resistance measurements. Automated measurement of tissue resistance in cell culture microplates provides important advantages: Drastically reduces time required to evaluate TEER on culture plates with HTS form factor Superior repeatability relative to manually acquired measurements  Significantly reduces the potential for measurement  error Compatible with major manufacturers of HTS culture plates Preconfigured at the factory to your choice of culture plate(s) Data automatically saved as text files which are easily imported into Excel The REMS AutoSampler automates TEER measurements previously made with WPI’s EVOM2 Epithelial Voltohmmeter. Automated tissue resistance measurements up to 20kΩ can be performed on 24- or 96-well HTS microplates. The REMS AutoSampler is compatible with Corning Costar HTS Transwell-24 and 96-well plates, Millipore MultiscreenTM CaCo 96-well plates and BD Falcon 96- and 24-well plates. The REMS AutoSampler automatically measures and records tissue resistance from a user-specified matrix of culture wells on the microplate. According to the specified sequence, the robotic arm moves into the identified wells taking TEER measurements. By means of a X-Y-Z locating system, the electrode arm is positioned precisely and reproducibly over each well. What's New? WPI just released a software upgrade for the REMS AutoSampler. The software runs on Windows 10. In addition to the operating system updates, the new software includes a host of new features, including: Dual rinse station locations and a calibration station location. The rinse station can be used to park the electrode while it is not in use. The system allows you to automate rinsing after each measurement, or you may pause the system at any time and rinse manually. Brand new interface that allows you to customize the name the background data files and the resistance data files from any tab. Safety features added to prevent damage to expensive probes. Simulation mode allows you to run the software without a robot connected. This works well for testing the software. Press the Stabilize button to see the resistance of the stabilizing well display on screen. New REMS AutoSampler
  9. REMS Robotic TEER Measurement for HTS
    April 08, 2014
    The REMS AutoSampler automates measurements of electrical resistance of transepithelial, transendothelial or Caco-2 cell membranes being grown to confluence on microporous filters of high throughput screening (HTS) 24- and 96-well microplates. It is a PC-controlled, tissue resistance measurement system that offers reproducibility, accuracy and flexibility for this kind of measurement. Automated measurement of tissue resistance in cell culture microplates provides the important advantages of speed, precision, decreased opportunity for contamination and the instant availability of measured resistance data on a computer. These measurements are useful in applications such as drug bioavailability studies and studies on the mechanisms of drug transport.
  10. FAQ: Troubleshooting Unstable Resistance Readings from an ENDOHM
    November 18, 2013
    One of our frequently asked questions (FAQs) concerns TEER measurements with an EndOhm. If the resistance readings from your ENDOHM don't stabilize, you may need to do some troubleshooting. Test the EVOM2 First, test your EVOM2 meter. The 1000Ω test resistor (WPI # 91750) can be used for this purpose.
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