Epithelial Volt/Ohm (TEER) Meter 3
TEER Measurement with automatic data logging
- Low noise design offers greater resolution and accuracy
- Automatic 20X sample averaging improves accuracy and stability
- Adjustable fixed measurement currents (2, 4 or 10 μA)
- Resistance auto ranging from 1 Ω to 100,000 Ω or with three fixed current ranges
- Reliable low current, low voltage design prevents metal ion transport
- Fast resistance stabilization on low levels under 200 Ω with resolution to 0.1 Ω
- Ergonomic tilt stand for low glare operation
- Graphical display of popular plates (6, 12, 24, 96) for trend analysis
- The display shows the most recent set of parameters
- Automatic plate indexing operation with or without control well subtraction for resistance and potential difference (PD) measurements
- Continuous data logging via USB (PC, Mac, Linux)
- Saves date stamped data to a spreadsheet readable file on a USB drive
- Upgradable firmware
- Eliminates errors and reduces experimental processing time
- Auto data logging eliminates the need to track data by hand
- The small footprint allows more bench space
- Easy calibration and verification
- Footswitch for hands-free recording
- Prevent data loss with auto save and data recovery when battery is low
- TEER is easily computed by applying a unit area formula to the resistance
- Measure epithelial or endothelial tissues for confluence, TEER and potential difference
- Permeability, conductance and drug studies
- Continuous digital monitoring of a target membrane
- Common studies
- Blood-brain barrier transport
- Lung epithelial tissue studies
- Intestinal tissue studies
- Skin studies
EVOM3 For Teer Measurement
The EVOM3 delivers improved workflow efficiency, more stable and repeatable measurements versus traditional Trans Epithelial Electrical Resistance (TEER) meters. Providing users with vital feedback during experiment measurements, the EVOM3’s large screen offers a range of informational views. The new graphical displays for trend analysis and measurement values helps scientists deliver simple, stepwise methodology during experimental measurements. The touch screen interface provides users an intuitive, easy-to-use menu for configuration.
Eliminating the need to log data by hand, the EVOM3 writes the resistance or voltage information to a USB drive in CSV format for easy transfer to spreadsheets and data analysis programs. When used with the footswitch it enables handsfree recording of measurements.
At the heart of the EVOM3 is our latest processor and circuitry, providing users with quick, easy and reliable readings due to its fast stabilization, automatic twenty times sampling average and low noise design. The auto ranging resistance feature allows for fast resistance measurements, and an over-range display feature eliminates false readings. The EVOM3 has adjustable current levels in three fixed ranges with two lower ranges for sensitive membranes and high resistance ranges up to 100 KΩ.
TEER Measurement Electrode
The STX2-PLUS electrode was designed for easy insertion into many 24-well plates. It is location re-placeable in the insert for repeatable and consistent measurements. The new shielded electrodes are now designed to minimize electrical interference and to be more easily maintained.
- STX2-PLUS new electrode designed for 12 and 24-well plates.
- Weighted self-standing electrode for hands-free stable measurements
- Shielded cable to minimize electrical and cell phone interference
|What is included with the EVOM3||QTY|
|EVOM3 Epithelial Volt Ohm Meter||1|
|STX2-PLUS Electrode set||1|
|300749 USB drive 32 GB (Used for storage. Also contains a Python 3.8 program for continuous digital monitoring of a target insert).||1|
|503535 USB cable||1|
|99673 Calibration kit, 1000Ω Test Resistor||1|
|803025 A/C power cord and charger||1|
|13142 Foot switch||1|
NOTE: A 99672 EVOM2 to EVOM3 Electrode Adapter is sold separately. The STX2, STX3 and all STX100s require the use of this adapter with the EVOM3.
- Keyed electrode base for repeatable placement gives more consistent results, eliminating the need for multiple readings.
- Easy to maintain
How Does the EVOM3 Work?
Confluence of a cellular monolayer is determined by an increase or a plateau in tissue resistance detected using the unique electronic circuit of the EVOM3 and the new STX2-PLUS electrode. The EVOM3 qualitatively measures cell monolayer health and quantitatively measures cell confluence. The EVOM3 produces a low AC current that avoids electrode metal deposits and adverse effects on tissues which can otherwise be caused by higher DC currents. The EVOM3 uses low current and voltages and is designed for non-destructive testing for epithelial monolayer confluence in cell cultures. In addition, resistance readings are unaffected by membrane capacitance or membrane voltage. The accuracy and repeatability of the EVOM3-STX2-PLUS system makes this instrument ideal for permeability, PD and other detailed membrane studies.
Electrodes For TEER (Epithelial) Measurement
|STX2-PLUS||Replacement Electrode Set|
|STX2*||Replacement Electrode Set (Requires 99672 for use with the EVOM3)|
|STX3*||Adjustable electrode set for shallow wells, 5-9 mm depth|
|3993*||2 mm Adapter for EVOM2|
*(Requires 99672 for use with the EVOM3)
ENDOHM Chambers For Endothelial/Epithelial Measurement
NEW EndOhm chambers include the EVOM3 cable 99916.
|ENDOHM-6G||EndOhm for 6 mm culture cup (24 wells per plate)|
|ENDOHM-12G||EndOhm for 12 mm culture cup (12 wells per plate)|
|ENDOHM-24G||EndOhm for 24 mm and Costar Snapwell cup (6 wells per plate)|
Software for EVOM3
EVOM3 firmware upgrade package EVOM3 Rev 1.5 (current release)
- Will the EVOM3 work with Endohm’s?
Yes, but the 99672 adaptor is required or the new EVOM3 cable 99916.
- Why would I want to use the blank function?
The blank feature is used when you want to subtract out any measurement that is not from the membrane, such as the electrode and fluid resistances.
- Does the EVOM3 system automatically calculate TEER?
No, TEER measurement requires an area calculation. To compute TEER, divide the measured resistance by the appropriate surface area (below). For example, a 12 mm insert measures 565 Ω, the TEER is 565 Ω/1.13 or 500 Ω. 6 well plate (24 mm inserts) 4.53 cm2, 12 well plate (12 mm inserts) 1.13 cm2, 24 well plate 6.5 mm inserts) 0.3316 cm2, 96 well plate (4.3 mm inserts) 0.143 cm2.
- EVOM3 data is stored automatically when the last well is reached. How do I store the data when I only want to measure 8 of 96 wells?
Clear any data in memory by opening settings, store menu then press new plate, that will clear any prior readings. Return to the main screen, open the preview screen, select each well to measure (the selection turns green), place the electrode, then measure. When you’re done measuring the selected wells, open the settings, press the store screen menu, then press store new to save the plate data to the USB drive.
This unit conforms to the following specifications:
|Tissue Sampling Frequency||12.5 Hz|
|Sample Averaging||20 samples per second|
|Auto Mode||1 to 100,000 Ω auto current 2 μA, 4 μA, 10 μA|
|Resistance Resolution||0.1 Ω (under 200 Ω); 1 Ω (over 200 Ω)|
|Voltage Resolution||0.001 V, 0.1 mV|
|Accuracy Resistance||0.1 Ω (200 Ω); 1 Ω (above 200 Ω)|
|Accuracy voltage||± 0.1 mV|
|Display Update Rate||0.5 seconds|
|Battery||3.7V Li-ion 2500 mAh**|
|Charging Period||5.5 hours (power off); 6 hours (run time)|
|Charge Current||200 mA|
|Power Consumption||~250 mA|
** mAH means milliamp hours.
Stanifer, M. L., et al. (2016). Reovirus intermediate subviral particles constitute a strategy to infect intestinal epithelial cells by exploiting TGF-β dependent pro-survival signaling. Cellular Microbiology, 18(12), 1831–1845.
Meenach, S. A., et al. (2016). Development of three-dimensional lung multicellular spheroids in air- and liquid-interface culture for the evaluation of anticancer therapeutics. International Journal of Oncology, 48(4), 1701–
Ferguson, M. C., et al. (2015). Ability of the Encephalitic Arbovirus Semliki Forest Virus To Cross the Blood-Brain Barrier Is Determined by the Charge of the E2 Glycoprotein. Journal of Virology, 89(15), 7536–7549. https://doi.
- Optional Foot Switch for Micro2T, Micro-ePore™, DMF100013142$152.00
- Electrode Set For EVOM399673$40.00
- USB2 Cable503535$36.00
- EVOM2 Mini XLR Adaptor99675$110.00
- EVOM3 Legacy Adaptor99672$45.00
- EVOM3 upgrade cable USB Mini-B803026$20.00
- EVOM3 EndOhm cable99916$180.00
- USB Drive 32GBMultiple SKUs
7 Reasons to Love the New EVOM3 for TEER Measurement
Why Choose an EVOM3 Over an EVOM2 for TEER Measurement