Microinjection Videos

[by Gabe Gonzalez]

The PV850 Injector is designed to simplify intracellular injection and a variety of other microinjection tasks. The PV850 uses regulated air pressure for injecting cells with fluid. Injected volumes range from picoliters to nanoliters. The port supplies positive pressure for high-pressure ejection maximum of 8 7PSI. The PV850 Microinjector offers separate regulated compensation (back filling prevention) and ejection pressures with a precision timing circuit that switches from injection pressure to compensation pressure automatically. Timing, injection pressure and compensation pressure are adjusted independently using the intuitive touch-screen user interface. Time intervals can range from 2 seconds down to 10 ms or less, depending on the injection pressure setting...more

Designed to simplify intracellular injection and a variety of other micro­in­jec­tion tasks, WPI’s PicoPumps (PV830 and PV820) use precisely regulated pres­sures for se­cur­ing cells and injecting them with fluid. Injected volumes range from picoliters to nanoliters. Separate ports supply positive and negative pressure—pos­i­tive pressure for high-pressure ejection, and suction for supporting the cell or for filling the pipette from the tip. In this quick series, you can see how to setup a PicoPump.

1. What's Included with a PV Pump

In this first video (#1) you can see all the components of the system as they unpack the system.

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[by Alec Dickson] 

WPI's FluoroDish™ tissue culture dishes provide exceptional imaging quality for many applications requiring the use of inverted microscopes such as high-resolution image analysis, microinjection and electrophysical recording of fluorescent-tagged cells. We have a 50 mm diameter dish and two types of 35 mm diameter dishes.

 

Better Optical Properties than Polycarbonate

Each WPI dish has a flat (0.17mm±0.01mm thick), optical quality glass bottom, allowing...more

The UltraMicroPump3 is a versatile microinjection syringe pump designed to support the needs of a busy lab. The UMP3 Microinjector is suitable for delivery of media from nanoliters to milliliters. the UMP3 injecotor’s user interface and system design enables delivery of highly accurate and repeatable injections. The choice for thousands of scientists, it is widely cited in research papers.

Intuitive Touchscreen Display for Easy Setup

The SMARTouch controller has an intuitive touch screen interface and an easy-to-use menu for quick configuration of the syringe size, volumes and flow...more

Do you need a precision microinjector with an integrated pressure source for injections in the picoliter range? WPI’s new MICRO-ePUMP, which is comparable to the Ependorf Femtojet, is a portable solution for injecting DNA, RNA and proteins, as well as pre- and post- implantation in embryos. Whether you are doing disease modeling with zebrafish or viral research for SARS-CoV2, the MICRO-ePUMP has much to offer. Here are eight good reasons to choose the MICRO-ePUMP for your applications.

1. Convenient internal pressure source
   The convenient internal pressure source is designed to simplify intracellular injection. With the carefully regulated internal pressure source, there is no need of an external air supply.
2. Intuitive...more

The new WPI MICRO-ePORE™ Pinpoint Cell Penetrator is a simple and versatile system that can be used for efficient microinjection of a diverse array of compounds and biomolecules into oocytes and pre-implantation stage mammalian embryos. Patent pending Flutter Electrode Technology assists in small, clean, precise membrane penetration without tearing or damaging the membrane. Here Gabe sets up the system and connects all the components.

Unpacking Your MICRO-ePORE™ System

Connecting Your MICRO-ePORE™ System

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World Precision Instruments' PUL-1000 is a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes used in intracellular recording, microperfusion and microinjection. It offers programmable sequences of up to four steps with complete control over the heating, force, movement and cooling time. This allows graduated cycles for a variety of applications. PUL-1000 can produce pipettes with tip diameters from less than 0.1µm to 10+ µm.

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WPI's Nanoliter 2010 Microinjection Pump is ideal for many applications, including zebrafish, xenopus oocytes and drosophila. MICRO4, an optional microprocessor-based controller, can provide an "intelligent" and easy-to-use interface to up to four Nanoliter Injectors. Operating parameters are set with the membrane key-pad and LCD display.

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Watch how researchers from the University of Chicago inject adult zebrafish using a 10μl NanoFil microsyringe controlled by a Micro4 controller and UltraMicroPump III (UMP3-1 includes one UMP3 pump and a Micro4 controller). 

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Chiara Cianciolo Cosentino, at the University of Pittsburgh, describes how she uses intravenous microinjections of zebrafish larvae to study acute kidney injury in this JoVE video. You can also watch this video on JoVE. WPI equipment shown in this video includes:

• MMP Manual Microsyringe Pump
• PV820 Pneumatic PicoPump
• M3301 Manual Micromanipulator
• M10 Magnetic Stand
• 5052 Steel Base Plate

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Researchers at the University of Michigan are using WPI's PV820 for injecting a morpholino solution into the lumen of the otic vesicle of 1-day old zebrafish embryos.  Then, they use electroporation to introduce mif and mif-like morpholinos into the developing inner ear tissues.

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In this video, Mike Pizza demonstrates how to front fill a Nanofil syringe using MicroFil.

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