A compact, versatile and reliable workhorse
- Program sequences up to four steps
- Produce micropipettes with a tip diameter less than 0.1 µm or greater than 10 µm
- Store up to 95 programs in memory for easy recall
- Two factory programs installed
- (1) PUL-1000 Puller
- (2) Glass clamp pads for 1.0~1.2 mm OD glass (translucent, pre-installed as default)
- (2) Glass clamp pads for 1.0~1.2 mm OD glass (translucent, spares)
- (2) Glass clamp pads for 1.5 mm OD glass (black)
- (2) Glass clamp pads for 2.0 mm OD glass (red)
- (1) Power supply
See the current Data Sheet.
- Tempered glass cover to reduce the effects of humidity on puller reproducibility
- Switchable power supply for any line voltage 90–240 VAC ensures that line voltage fluctuations don’t affect reproducibility
- Pull your own microelectrodes and micropipettes
PUL-1000 is a microprocessor controlled horizontal puller for making glass micropipettes or microelectrodes used in intracellular recording, patch clamp studies, microperfusion or microinjection. The puller was designed with tight mechanical specifications and precision electronics for complete control of the pulling process and accurate reproducibility. It offers programmable sequences of up to four steps with heating, force, movement and cooling time. This allows graduated cycles for applications like patch clamp recording.
This puller is a reasonably priced, compact, versatile and reliable workhorse. The microprocessor, combined with the LCD display, makes the PUL-1000 easy to use.Tempered glass cover
The cover of the pulling chamber is made with tempered glass to minimize the temperature effect on the reproducibility of the pulled pipettes.
Switchable power supply
PUL-1000 has a high quality switching power supply for use anywhere in the world without worry about the line voltage differences. Pulling reproducibility is unaffected by line voltage fluctuation. Heating voltage can be controlled to within 0.1% accuracy even when line voltage fluctuates from 90 to 240 VAC.
The settings for both stages can be stored in memory. Up to 95 user-selectable programs can be stored for later recall. The instrument contains two factory installed and tested programs. Choose from the factory installed programs or create your own.
A glass capillary is heated by a platinum/iridium filament and pulled by a controlled force. PUL-1000 features permanent memory storage for up to 95 heat programs. It is remarkable in the flexibility and capability of producing a vast array of pipette shapes.
Pulling pipettes is an art, and reliable results depend on factors like the operating environment, the type of glass used and your technique. Understanding how the puller works is critical to manufacturing the pipettes you want.
PUL-1000 can produce pipettes with tip diameters from less than 0.1µm to 10+ µm. Microprocessor settings control the pulling automatically.
Choosing a Filament
Appropriate filament selection depends on your research application, but generally Box Filaments are recommended. This configuration is particularly suitable for slice preparations where long, parallel walls will aid penetration. If you are using a box filament, the size of the square box should be approximately 1.0mm to 1.5mm larger than the outside diameter of the glass to be pulled.
|13834||Replacement box filament, 2.5 mm square, platinum iridium, 2.5 mm wide (Installed by default)|
|14074||Replacement box filament, 3 mm square, platinum iridium, 2 mm wide|
Compare all the pullers, bevelers, microforges (application guide).
|Heater Element||Platinum/Iridium Filament|
|Pulling Force||Solenoid, adjustable|
|Taper Length||1–10 mm|
|Capillary OD Range||1.0–2.0 mm*|
|Maximum Capillary Length||170 mm|
|Minimum Capillary Length||55 mm|
|Permanent Memory Set||30|
|Power||90–240 VAC, 50/60 Hz, Max. 70 W|
|Replacement Filaments||13834 2.5mm Square Box Filament, 2.5mm wide|
|DIMENSIONS||34 x 24 x 12 cm (13.4 x 9.4 x 4.7")|
|WEIGHT||15 lb. (7 kg)|
*Use the white pad (default) with 1-1.2 mm glass, the black pad with 1.5 mm glass and the red pad with 2 mm glass.
Plautz, C. Z., Williams, H. C., & Grainger, R. M. (2016). Functional Cloning Using a <em>Xenopus</em> Oocyte Expression System. Journal of Visualized Experiments, (107), e53518–e53518. http://doi.org/10.3791/53518
Komarova, Y., Peloquin, J., & Borisy, G. (2011). Components of a microinjection system. Cold Spring Harbor Protocols, 2011(8), 935–9. http://doi.org/10.1101/pdb.ip27
The video below gives a brief overview of this microelectrode/micropipette puller and its features.