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EVOM
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July 12, 2022
WPI’s EVOM™ technology is the gold standard for delivering stable and repeatable Trans Epithelial Electrical Resistance (TEER) measurements. Here are nine reasons to trust the new EVOM Manual TEER Meter for qualitatively measuring cell monolayer health and quantitatively measuring cell confluence.
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May 31, 2022
WPI’s EVOM™ Manual is the gold standard for delivering stable and repeatable Trans Epithelial Electrical Resistance (TEER) measurements. The EVOM™ Manual qualitatively measures cell monolayer health and quantitatively measures cell confluence by determining an increase or a plateau in tissue resistance detected using our innovative EVOM™ technology. The main display window has lots of information. Here’s a quick overview. You can download the infographic for a handy reference.
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May 24, 2022
WPI’s EVOM™ Manual is the gold standard for delivering stable and repeatable Trans Epithelial Electrical Resistance (TEER) measurements. The EVOM™ Manual is the newest TEER meter. It qualitatively measures cell monolayer health and quantitatively measures cell confluence by determining an increase or a plateau in tissue resistance detected using our innovative EVOM™ technology. WPI’s state of the art EVOM™ technology provides you with real-time, valuable feedback during experimental measurements.
Electrode options are available for use with the new EVOM™ Manual. Here we will compare these options so you can choose one that’s right for your application.
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May 20, 2022...more
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May 05, 2022
REMS AutoSampler is an automated TEER measurement system that uses a robotic arm to make measurements in 24- and 96-well plates. This article details how to upgrade the software for a REMS system.
Considerations Before You Begin
The first consideration is the hardware in your REMS system and the software version you are running.
Systems Older than 2014
- If your system was purchased before 2014, the REMS will have a PCI6023 data card, and you may only upgrade to the REMS4 software. See below for instructions on verifying your software version.
- To upgrade to the current version of the REMS software (REMS6.02), a unit purchase before 2014 will need a PCI6220 data card. You may purchase the upgrade kit (WPI# 300555), which includes the board/data card (for example, NI PCI6220), a new interface cable and software version 6.0.2.
In addition, you need a newer computer with free PCI slot. WPI’s current REMS computer...more
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April 20, 2021
by Benjamin Dubansky, PhD, Animal Physiologist/Researcher
The EVOM is a special kind of voltmeter for measuring electrical properties across a layer of cells in culture or a biological membrane. Here's a few things I like about the new EVOM3.
There's a couple things I like about the EVOM3 better than the older models.
- You can charge it. These ones had batteries which are fine, and they had a power plug. But when you use the power plug, you get a little bit electrical noise, and the signal's not quite as stable. When you have a good battery in here, the charge lasts for quite some time. It charges pretty quickly. You...more
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April 20, 2021
by Benjamin Dubansky, PhD, Animal Physiologist/Researcher
An Ussing Chamber is used when performing barrier studies across an excised epithelial tissue. Similar measurements can be made in well plates using an EVOM series TEER Measurement Meter, which is used for epithelial tissue that is grown to confluence in a cell culture well. While EVOM measurements are only qualitative, your throughput is much greater with an EVOM3. The EVOMs are portable and much more economical than an Ussing Chamber. Here Ben Dubansky, PhD explains the differences between EVOMs and Ussing chambers.
The EVOM is a special kind of voltmeter for measuring electrical properties across a layer of cells in culture or a biological membrane. We are measuring TEER (TER) - Transepithelial Electrical Resistance. The EVOM3...more
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April 19, 2021
by Benjamin Dubansky, PhD, Animal Physiologist/Researcher
The EVOM is a special kind of voltmeter for measuring electrical properties across a layer of cells in culture or a biological membrane. It is the most commonly used commercial system for measuring across a layer of cultured cells. We are measuring TEER (TER) - Transepithelial Electrical Resistance. The EVOM3 allows us to zoom in and measure either resistance or voltage across these membranes or cells in culture.
Why was it invented?
The EVOM (Epithelial Volt Ohm Meter) was designed in the 80’s. It was invented back when multiwell culture plates were first introduced...more
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May 02, 2019
EndOhm Chambers Re-engineered
EndOhm chambers are designed for making accurate TEER measurement of epithelial and endothelial cell cultures. The design keeps the top and bottom electrodes at a fixed gap and maintains the sample (cell culture insert) in a centered position inside. This design minimizes the variability associated with electrode positioning and gap, making EndOhm chambers more accurate and capable of generating reproducible results. WPI made some recent upgrades to the EndOhm chambers. - The NEW EndOhm Chamber is made of GLASS, making it easier to clean and more...more
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November 28, 2018
Selection of Electrodes for TEER Measurements
Transepithelial electrical resistance (TEER), also referred as the transepithelial resistance (TER) is used to monitor cellular health. TEER is comprised of measurements of the transcellular pathway (i.e., resistance due to an individual cell) and paracellular pathway (i.e., resistance due to the formation of the cellular junctions). TEER is commonly used to monitor cellular confluence. TEER values can indicate changes in the cellular monolayer permeability, showing the monolayer barrier function of cells such as, endothelial (brain microvessel) and epithelial (alveolar, kidney, and intestinal) cells. High TEER values generally reflect tighter cellular monolayers or cellular junctions (Lewis 1996, Matter and Balda 2003, Denker and Sabath 2011). A few major benefits of WPI TEER measurement systems are described below. The TEER values (electrophysiological analysis) can be combined with other analysis methods to further understand...more
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February 02, 2015
Here are some frequently asked questions (FAQ) about TEER measurement using an EVOM2.
- Are the electrical resistance and transepithelial electrical resistance (TEER) the same thing?
- What is an EndOhm chamber?
- How is an EVOM2 used for measuring confluence?
- Why use an EndOhm instead of a STX?
- How do I clean my electrode?
- What about electrode preconditioning?
- Can you give me a simple data acquisition system for TEER?
- What are the TEER measurement challenges that I may encounter?
- Can you suggest some experimental...more
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November 11, 2014
The EVOM2 is the classic, hand-held instrument for making Trans Epithelial Electrical Resistance (TEER) measurements. The REMS system adds a robot and data recording for automated TEER measurements. There may be times when you don't have a robot, but would still like to have the data recording capabilities of the REMS system. With a little ingenuity, you can do just that. Here, we will show you how you can add data recording to your standard EVOM2...more.
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November 18, 2013
One of our frequently asked questions (FAQs) concerns TEER measurements with an EndOhm. If the resistance readings from your ENDOHM don't stabilize, you may need to do some troubleshooting. Test the EVOM2
First, test your EVOM2 meter. The 1000Ω test resistor (WPI # 91750) can be used for this purpose.
- Insert the RJ-11 plug at the end of the test resistor into the Input port on the meter. ...more
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October 11, 2013
With the development of a High Throughput Screening (HTS) protocol for faster drug discovery, a new line of cell culture filter plates have been introduced by several major cell culture insert manufacturers. These HTS plates normally have either 24 or 96 individual cell culture inserts "bonded" together as one plate so that it can be handled by a robot apparatus. In response to these developments, WPI has developed an automatic REMS system and a manual electrode, STX100, for TEER measurements using HTS plates. - Designed for use with 24-well HTS plates (Corning Costar and BD Falcon) and with 96-well plates (Millipore and BD Falcon)
- Improved accuracy down to 5Ω
- Sterilize with EtO, alcohol or bactericide
STX100's design is based on the same reliable design principle as the universally...more
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October 11, 2013
Transepithelial Electrical Resistance TEER measurements is the most convenient, reliable and non-destructive method for evaluating and monitoring the growth of epithelial tissue cultures in vitro. The confluence of the cellular monolayer is quickly determined by a sharp increase in TEER. TEER measurement technology, which was first introduced by WPI in the mid-1980's, has since been perfected and expanded to include a range of TEER related manual and automatic instrumentation, including:
- EVOM² - Manual TEER measurement of epithelial cells in 24- and 96-well plates
- REMS AutoSampler - Automated system for High Throughput Screening (HTS)
EVOM2 Volt-Ohm Meter
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