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Epithelial Physiology
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July 12, 2022
WPI’s EVOM™ technology is the gold standard for delivering stable and repeatable Trans Epithelial Electrical Resistance (TEER) measurements. Here are nine reasons to trust the new EVOM Manual TEER Meter for qualitatively measuring cell monolayer health and quantitatively measuring cell confluence.
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July 11, 2022
EVOM™ Manual is WPI’s newest instrument to measure Trans Epithelial Electrical Resistance (TEER). Let’s look at how to set it up and take resistance measurements.
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Power on the EVOM™ Manual using the power switch on the rear panel.
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Insert the USB flash drive provided with the system into the USB port on the side of the meter.
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By default, the filename is plate1. You may choose a prefix that auto-indexes or a unique file name:...more
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May 31, 2022
WPI’s EVOM™ Manual is the gold standard for delivering stable and repeatable Trans Epithelial Electrical Resistance (TEER) measurements. The EVOM™ Manual qualitatively measures cell monolayer health and quantitatively measures cell confluence by determining an increase or a plateau in tissue resistance detected using our innovative EVOM™ technology. The main display window has lots of information. Here’s a quick overview. You can download the infographic for a handy reference.
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May 24, 2022
WPI’s EVOM™ Manual is the gold standard for delivering stable and repeatable Trans Epithelial Electrical Resistance (TEER) measurements. The EVOM™ Manual is the newest TEER meter. It qualitatively measures cell monolayer health and quantitatively measures cell confluence by determining an increase or a plateau in tissue resistance detected using our innovative EVOM™ technology. WPI’s state of the art EVOM™ technology provides you with real-time, valuable feedback during experimental measurements.
Electrode options are available for use with the new EVOM™ Manual. Here we will compare these options so you can choose one that’s right for your application.
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May 20, 2022...more
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May 05, 2022
REMS AutoSampler is an automated TEER measurement system that uses a robotic arm to make measurements in 24- and 96-well plates. This article details how to upgrade the software for a REMS system.
Considerations Before You Begin
The first consideration is the hardware in your REMS system and the software version you are running.
Systems Older than 2014
- If your system was purchased before 2014, the REMS will have a PCI6023 data card, and you may only upgrade to the REMS4 software. See below for instructions on verifying your software version.
- To upgrade to the current version of the REMS software (REMS6.02), a unit purchase before 2014 will need a PCI6220 data card. You may purchase the upgrade kit (WPI# 300555), which includes the board/data card (for example, NI PCI6220), a new interface cable and software version 6.0.2.
In addition, you need a newer computer with free PCI slot. WPI’s current REMS computer...more
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April 20, 2021
by Benjamin Dubansky, PhD, Animal Physiologist/Researcher
The EVOM is a special kind of voltmeter for measuring electrical properties across a layer of cells in culture or a biological membrane. Here's a few things I like about the new EVOM3.
There's a couple things I like about the EVOM3 better than the older models.
- You can charge it. These ones had batteries which are fine, and they had a power plug. But when you use the power plug, you get a little bit electrical noise, and the signal's not quite as stable. When you have a good battery in here, the charge lasts for quite some time. It charges pretty quickly. You...more
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April 20, 2021
by Benjamin Dubansky, PhD, Animal Physiologist/Researcher
An Ussing Chamber is used when performing barrier studies across an excised epithelial tissue. Similar measurements can be made in well plates using an EVOM series TEER Measurement Meter, which is used for epithelial tissue that is grown to confluence in a cell culture well. While EVOM measurements are only qualitative, your throughput is much greater with an EVOM3. The EVOMs are portable and much more economical than an Ussing Chamber. Here Ben Dubansky, PhD explains the differences between EVOMs and Ussing chambers.
The EVOM is a special kind of voltmeter for measuring electrical properties across a layer of cells in culture or a biological membrane. We are measuring TEER (TER) - Transepithelial Electrical Resistance. The EVOM3...more
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April 19, 2021
by Benjamin Dubansky, PhD, Animal Physiologist/Researcher
The EVOM is a special kind of voltmeter for measuring electrical properties across a layer of cells in culture or a biological membrane. It is the most commonly used commercial system for measuring across a layer of cultured cells. We are measuring TEER (TER) - Transepithelial Electrical Resistance. The EVOM3 allows us to zoom in and measure either resistance or voltage across these membranes or cells in culture.
Why was it invented?
The EVOM (Epithelial Volt Ohm Meter) was designed in the 80’s. It was invented back when multiwell culture plates were first introduced...more
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May 15, 2020
The NEW EVOM3 Epithelial Volt/Ohm Meter delivers improved workflow efficiency, increased stability and more repeatable measurements than traditional Trans Epithelial Electrical Resistance (TEER) meters. Here are seven things you will love about the new EVOM3.
1. It has an intuitive Touchscreen Display
Providing users with vital feedback during experimental measurements, the EVOM3’s large touch screen offers a range of informational views. The new graphical displays for trend analysis and measurement values helps you deliver a simple, stepwise methodology during experimental measurements. The touch screen interface...more
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April 13, 2020
Epithelial and endothelial cells are known for their barrier function like selective permeability. Lung tissue is comprised of epithelial cellular layers, residing adjacent to endothelial layers and allowing exchange of oxygen and carbon dioxide between lung and blood. Currently, the biomedical research community’s major focus area is to understand the details...more
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May 02, 2019
EndOhm Chambers Re-engineered
EndOhm chambers are designed for making accurate TEER measurement of epithelial and endothelial cell cultures. The design keeps the top and bottom electrodes at a fixed gap and maintains the sample (cell culture insert) in a centered position inside. This design minimizes the variability associated with electrode positioning and gap, making EndOhm chambers more accurate and capable of generating reproducible results. WPI made some recent upgrades to the EndOhm chambers. - The NEW EndOhm Chamber is made of GLASS, making it easier to clean and more...more
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February 22, 2019
WPI EndOhm chambers are used with WPI's EVOM2 meter for making TEER (transepithelial electrical resistance) measurements. Here Subhra shows the difference between hanging cell culture inserts and standing cell culture inserts.
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February 21, 2019
The WPI EVOM2 meter is used to measure TEER (transepithelial electrical resistance) in cell culture inserts. The STX2 electrodes with a static length may cause damage to Snapwell inserts. Here Subhra demonstrates how to use the adjustable STX3 electrode to prevent damage to your cell culture inserts.