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Epithelial Physiology

  1. Monitor the Growth of Epithelial Cells in Vitro Manually
    November 20, 2015
    For small batches of cell culture Are you ready to perform experiments involving a cultured cell monolayer? If your monolayer has not reached confluence, you may have holes or gaps in the monolayer that would prevent collection of valid experimental data. When confluence is reached, the electrical resistance across the monolayer peaks and then plateaus. The EVOM2 meter was designed specifically for this application. The EVOM2 is the ideal meter for use when you are making manual TEER measurements on small batches of cell cultures and do not require a high throughput system. EVOM2 - Perfect for TEER The EVOM2 was designed to measure TEER resistance without damaging the epithelial cells. It passes a constant current
  2. Setting Up Your Automated TEER Measurement Robot
    April 14, 2015
    Now that your REMS system has arrived, are you trying to figure out how to set it up? Check out this 5-minute video where we show you how, step-by-step. More Info
  3. FAQs about TEER Measurement
    February 02, 2015
    Here are some frequently asked questions (FAQ) about TEER measurement using an EVOM2. Are the electrical resistance and transepithelial electrical resistance (TEER) the same thing? What is an EndOhm chamber? How is an EVOM2 used for measuring confluence? Why use an EndOhm instead of a STX? How do I clean my electrode? What about electrode preconditioning? Can you give me a simple data acquisition system for TEER? What are the TEER measurement challenges that I may encounter? Can you suggest some experimental parameters that can be controlled to obtain consistent TEER results?
  4. Recording TEER Measurements from an EVOM2
    November 11, 2014
    The EVOM2 is the classic, hand-held instrument for making Trans Epithelial Electrical Resistance (TEER) measurements. The REMS system adds a robot and data recording for automated TEER measurements. There may be times when you don't have a robot, but would still like to have the data recording capabilities of the REMS system. With a little ingenuity, you can do just that. Here, we will show you how you can add data recording to your standard EVOM2
  5. REMS High Throughput TEER Robot Gets Software Upgrade
    May 30, 2014
    What is REMS? The REMS AutoSampler automates measurements of electrical resistance of transepithelial, transendothelial or Caco-2 cell membranes being grown to confluence on microporous filters of high throughput screening (HTS) 24- and 96-well microplates. It is a PC-controlled, tissue resistance measurement system that offers reproducibility, accuracy and flexibility for this kind of measurement. The ability of the REMS AutoSampler to precisely locate the electrode results in highly reproducible TEER measurements. TEER  measurements are stored in the computer as the electrode moves from one well to the next. The Windows-based
  6. REMS Robotic TEER Measurement for HTS
    April 08, 2014
    The REMS AutoSampler automates measurements of electrical resistance of transepithelial, transendothelial or Caco-2 cell membranes being grown to confluence on microporous filters of high throughput screening (HTS) 24- and 96-well microplates. It is a PC-controlled, tissue resistance measurement system that offers reproducibility, accuracy and flexibility for this kind of measurement. Automated measurement of tissue resistance in cell culture microplates provides the important advantages of speed, precision, decreased opportunity for contamination and the instant availability of measured resistance data on a computer. These measurements are useful in applications such as drug bioavailability studies and studies on the mechanisms of drug transport.
  7. FAQ: Troubleshooting Unstable Resistance Readings from an ENDOHM
    November 18, 2013
    One of our frequently asked questions (FAQs) concerns TEER measurements with an EndOhm. If the resistance readings from your ENDOHM don't stabilize, you may need to do some troubleshooting. Test the EVOM2 First, test your EVOM2 meter. The 1000Ω test resistor (WPI # 91750) can be used for this purpose. Insert the RJ-11 plug at the end of the test resistor  into t
  8. STX100 Electrodes TEER Measurement
    October 11, 2013
    With the development of a High Throughput Screening (HTS) protocol for faster drug discovery, a new line of cell culture filter plates have been introduced by several major cell culture insert manufacturers. These HTS plates normally have either 24 or 96 individual cell culture inserts "bonded" together as one plate so that it can be handled by a robot apparatus. In response to these developments, WPI has developed an automatic REMS system and a manual electrode, STX100, for TEER measurements using HTS plates. Designed for use with 24-well HTS plates (Corning Costar and BD Falcon) and with 96-well plates (Millipore and BD Falcon) Improved accuracy down to
  9. TEER Research: WPI's Manual and Robotic Systems
    October 11, 2013
    Transepithelial Electrical Resistance TEER measurements is the most convenient, reliable and non-destructive method for evaluating and monitoring the growth of epithelial tissue cultures in vitro. The confluence of the cellular monolayer is quickly determined by a sharp increase in TEER. TEER measurement technology, which was first introduced by WPI in the mid-1980's, has since been perfected and expanded to include a range of TEER related manual and automatic instrumentation, including: EVOM² - Manual TEER measurement of epithelial cells in 24- and 96-well plates REMS AutoSampler - Automated system for High Throughput Screening (HTS) EVOM2 Volt-Ohm Meter
  10. REMS Auto Sampler - HTS Robot
    October 11, 2013
    The REMS AutoSampler automates measurements of electrical resistance of transepithelial, transendothelial or Caco-2 cell membranes being grown to confluence on microporous filters of high throughput screening (HTS) 24- and 96-well microplates. It is a computer-controlled, tissue resistance measurement system that offers reproducibility, accuracy, flexibility and ease-of-operation for this kind of measurement. Automated measurement of tissue resistance in cell culture microplates provides the important advantages of speed, precision, decreased opportunity for contamination and the instant availability of measured resistance data on a computer. These measurements are useful in applications such as drug bioavailability studies and studies on the mechanisms of
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