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February 11, 2021M3301 is a popular manual micromanipulator at WPI. These manipulators are typically mounted on a stand or table to ensure stability. In this video we show you how to mount the manipulator on an Tilt Base (WPI# M-3) with a WPI# 5464 5 lb. weighted base. When we mount a micromanipulator on an M-3 tilt base, the tilt base can be adjusted to position the micromanipulator right where you want it. We will use our popular M3301 micromanipulator to demonstrate. The first thing we need to do is remove the ring clamp from the bottom of the micromanipulator. There are two screws that -
February 10, 2021Over time, you may experience mechanical drift in one or more of the axes of your manual micromanipulator. Here we show you how to adjust for that. The process is similar for other styles of manual micromanipulators. We will look at the process for adjusting all three axes of a KITE micromanipulator. Let's talk about mechanical drift on a micromanipulator and how to correct it. In this demonstration, we will use the popular KITE. A manipulator axis may begin to move gradually under its own weight, even if it's not being touched. This is called mechanical drift, and it happens from normal use of the manipulator over time. Each course axis control on the micromanipulator has its own adjustments. This procedure is very similar to the one we used for adjusting an M3301 micromanipulator. It also -
February 09, 2021Magnetic stands act as an invaluable extra hand around the laboratory to hold an electrode, digital dials or tools. The base houses a strong magnet which can be activated by turning the switch. Position the magnetic stand on a weighted steel base plate or metal desk and rotate the dial counter-clockwise to engage the magnet. We offer multiple styles of magnetic stands, but these videos show a couple options. Flexible Magnetic Stand The M11 Flexible Magnetic Stand is ideal for probe holders and other lightweight objects. The flexible arm bends like a snake and locks into position with a flick of the control lever. Here we show you how easy it is to set it up. Magnetic Stand with Adjustment Arm The M9 Magnetic Stand has an adjustment arm. We'll show you how to easily position it and set it in place. The magnetic base can be easily engaged or disengaged so that you can mount the stand firmly to a metal desk or weighted metal base plate. See All Magnetic Stands -
February 02, 2021WPI pre-pulled pipettes (MicroTips) are a popular choice for pharmaceutical companies carrying out CCIT (Closed Container Integrity Testing). Each MicroTip is individually tested and inspected and can be shipped with a certificate of compliance. CCIT is an important part in understanding the biological safety and suitability of primary packaging. It is used to assess the potential of the container closure system to maintain a sterile barrier or to prevent leakage resulting in contamination or loss of the drug. A good overview of Understanding Container -
January 29, 2021The system depicted includes components often favored by researchers: MICRO-ePUMP Pneumatic PicoPump with built in MICRO-ePORE™ Cell Penetrator to facilitate microinjections SU-P1000 Micropipette Puller M4C Stand M3301R Micromanipulator PZMTIII Microscope with Optional Lighted Base with Articulating Mirror and optional PRO-300 HDS Camera and View Screen E2XX Micropipette Storage Jar Z-MOLDS Microinjection and Transplantation Molds 14003-G Vannas spring scissors Glass Capillaries 77020 Glass Tweezers FluoroDish Optical Grade Glass Bottom Culture Dishes Whatever your needs, WPI offers a range of equipment to fill your requirements. Download Microinjection Systems Brochure Empowering Scientists with Reliable Instruments Serving scientists for over 50 years, WPI offers a variety of instruments for microinjection including pumps, pipetters, microscopes and more. One of our most popular pumps for microinjection is the PV850 Pneumatic PicoPump. The PV850, PV830, uPump and MICRO-ePUMP were designed to simplify intracellular injection. You get repeatable microinjection in volumes ranging from picoliters to nanoliters. The volume injected is controlled by the inside diameter of the glass tip, the pressure and the time. The PV850 offers eject and hold pressure. The hold pressure prevents backfilling of the pipette by capillary action, ensuring repeatable injections. In addition, the PV830 also has vacuum pressure which allows you to securely hold a cell with one pipette while you inject it with another. The uPUMP and MICRO-ePUMP have a built in pressure source, eliminating the need for an external pressure supply. The MICRO-ePUMP with Integrated Cell Penetrator and Internal Pressure Source is perfect for intracellular injection. The Cell Penetrator delivers a highly localized voltage signal to a targeted injection site to facilitate penetration with minimal trauma. WPI h -
January 28, 2021World Precision Instruments (WPI) offers a broad range of research instruments for zebrafish (Danio Rerio) research, from the time the genetics of the subject are modified through the point where the physiological effects of the change are monitored. Whether researchers are performing microinjection or making cardiovascular measurements, the WPI product line includes instruments for research on oocytes, embryos, larvae and adult zebrafish. Enhanced Microinjection Efficiency for Genetic Modification The customizable Zebrafish Microinjection Toolbox -
September 08, 2020[by Gabe Gonzalez] The PV850 Injector is designed to simplify intracellular injection and a variety of other microinjection tasks. The PV850 uses regulated air pressure for injecting cells with fluid. Injected volumes range from picoliters to nanoliters. The port supplies positive pressure for high-pressure ejection maximum of 8 7PSI. The PV850 Microinjector offers separate regulated compensation (back filling prevention) and ejection pressures with a precision timing circuit that switches from injection pressure to compensation pressure automatically. Timing, injection pressure and compensation pressure are adjusted independently using the intuitive touch-screen user interface. Time intervals can range from 2 seconds down to -
September 08, 2020[by Gabe Gonzalez] The PV850 Injector is designed to simplify intracellular injection and a variety of other microinjection tasks. The PV850 uses regulated air pressure for injecting cells with fluid. Injected volumes range from picoliters to nanoliters. The port supplies positive pressure for high-pressure ejection maximum of 87 PSI. The PV850 Microinjector offers separate regulated compensation (back filling prevention) and ejection pressures with a precision timing circuit that switches from injection pressure to compensation pressure automatically. Timing, injection pressure and compensation pressure are adjusted independently using the intuitive touch-screen user interface. Time intervals can range from 2 seconds down to -
September 03, 2020Designed to simplify intracellular injection and a variety of other microinjection tasks, WPI’s PicoPumps (PV830 and PV820) use precisely regulated pressures for securing cells and injecting them with fluid. Injected volumes range from picoliters to nanoliters. Separate ports supply positive and negative pressure—positive pressure for high-pressure ejection, and suction for supporting the cell or for filling the pipette from the tip. In this quick series, you can see how to setup a PicoPump. 1. What's Included with a PV Pump In this first video (#1) you can see all the components of the system as they unpack the system.
