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REMS

  1. Setting Up Your Automated TEER Measurement Robot
    April 14, 2015
    Now that your REMS system has arrived, are you trying to figure out how to set it up? Check out this 5-minute video where we show you how, step-by-step. More Info
  2. Recording TEER Measurements from an EVOM2
    November 11, 2014
    The EVOM2 is the classic, hand-held instrument for making Trans Epithelial Electrical Resistance (TEER) measurements. The REMS system adds a robot and data recording for automated TEER measurements. There may be times when you don't have a robot, but would still like to have the data recording capabilities of the REMS system. With a little ingenuity, you can do just that. Here, we will show you how you can add data recording to your standard EVOM2. Open Labscribe3 on your computer. Let the program identify the data recorder. In our example, we will use a Lab-Trax-4. Choose the default settings. Setup the preferences for one channel. If you like, you may add five points of smoothing.  In the marks section of the main window, select A1 (channel one). This records a data point directly into the marks records window. Alternatively, the smoothed graph C1 may be selected. In the top graph, right click and choose Units>Simple to calibrate the graph to ohms. Enter these values for the EVOM2 conversion: 2 point calibration Apply units to new data Value at Cursor 1: 0.0000 => 0.0000 Value at Cursor 2: 1.0000 => 1000 Unit Name = Ohms To connect the EVOM2 to the data recorder, connect a BNC cable from the Output BNC on the front of the EVOM2 to the Channel 1 input BNC on your data recorder. In the Marks window, enter A1 or Control. Move the electrode to the control well, and allow it to stabilize. Then, press Enter. Otherwise, it is difficult to hold the electrodes still while typing. For each additional well, enter in the number A2…, A3 into the Marks field. Then, move the electrode to that well and press the enter key after the reading stabilizes. Stop the recording and save the file. Open up the marks editor in Labscribe (View>Marks>Marks). The dialog box shows the time mark data and resistance values.   Press the Export button and choose “All Marks.” Give your export file a name. The export files is a text file that may be import into Microsoft Excel or another spreadsheet application.
  3. REMS High Throughput TEER Robot Gets Software Upgrade
    May 30, 2014
    What is REMS? The REMS AutoSampler automates measurements of electrical resistance of transepithelial, transendothelial or Caco-2 cell membranes being grown to confluence on microporous filters of high throughput screening (HTS) 24- and 96-well microplates. It is a PC-controlled, tissue resistance measurement system that offers reproducibility, accuracy and flexibility for this kind of measurement. The ability of the REMS AutoSampler to precisely locate the electrode results in highly reproducible TEER measurements. TEER measurements are stored in the computer as the electrode moves from one well to the next. The Windows-based software provides user-friendly features to acquire, display and store the tissue resistance measurements. Automated measurement of tissue resistance in cell culture microplates provides important advantages: Drastically reduces time required to evaluate TEER on culture plates with HTS form factor Superior repeatability relative to manually acquired measurements  Significantly reduces the potential for measurement  error Compatible with major manufacturers of HTS culture plates Preconfigured at the factory to your choice of culture plate(s) Data automatically saved as text files which are easily imported into Excel The REMS AutoSampler automates TEER measurements previously made with WPI’s EVOM2 Epithelial Voltohmmeter. Automated tissue resistance measurements up to 20kΩ can be performed on 24- or 96-well HTS microplates. The REMS AutoSampler is compatible with Corning Costar HTS Transwell-24 and 96-well plates, Millipore MultiscreenTM CaCo 96-well plates and BD Falcon 96- and 24-well plates. The REMS AutoSampler automatically measures and records tissue resistance from a user-specified matrix of culture wells on the microplate. According to the specified sequence, the robotic arm moves into the identified wells taking TEER measurements. By means of a X-Y-Z locating system, the electrode arm is positioned precisely and reproducibly over each well. What's New? WPI just released a software upgrade for the REMS AutoSampler. The software runs on Windows 10. In addition to the operating system updates, the new software includes a host of new features, including: Dual rinse station locations and a calibration station location. The rinse station can be used to park the electrode while it is not in use. The system allows you to automate rinsing after each measurement, or you may pause the system at any time and rinse manually. Brand new interface that allows you to customize the name the background data files and the resistance data files from any tab. Safety features added to prevent damage to expensive probes. Simulation mode allows you to run the software without a robot connected. This works well for testing the software. Press the Stabilize button to see the resistance of the stabilizing well display on screen. New REMS AutoSampler
  4. REMS Robotic TEER Measurement for HTS
    April 08, 2014
    The REMS AutoSampler automates measurements of electrical resistance of transepithelial, transendothelial or Caco-2 cell membranes being grown to confluence on microporous filters of high throughput screening (HTS) 24- and 96-well microplates. It is a PC-controlled, tissue resistance measurement system that offers reproducibility, accuracy and flexibility for this kind of measurement. Automated measurement of tissue resistance in cell culture microplates provides the important advantages of speed, precision, decreased opportunity for contamination and the instant availability of measured resistance data on a computer. These measurements are useful in applications such as drug bioavailability studies and studies on the mechanisms of drug transport.
  5. REMS Auto Sampler - HTS Robot
    October 11, 2013
    The REMS AutoSampler automates measurements of electrical resistance of transepithelial, transendothelial or Caco-2 cell membranes being grown to confluence on microporous filters of high throughput screening (HTS) 24- and 96-well microplates. It is a computer-controlled, tissue resistance measurement system that offers reproducibility, accuracy, flexibility and ease-of-operation for this kind of measurement. Automated measurement of tissue resistance in cell culture microplates provides the important advantages of speed, precision, decreased opportunity for contamination and the instant availability of measured resistance data on a computer. These measurements are useful in applications such as drug bioavailability studies and studies on the mechanisms of drug transport. The main components of the REMS AutoSampler include: Robotic sampler that moves the electrode over each well of the microplate Electrode which is located on the robotic arm Base plate for the 24-and 96-well tray Windows-based data acquisition card REMS interface unit REMS software to operate the system on a Windows-based computer The REMS AutoSampler automates TEER measurements previously made with WPI's EVOM2 Epithelial Voltohmmeter. Automated tissue resistance measurements up to 20kΩ can be performed on 24- or 96-well HTS microplates. Microplates presently supported include the Corning Costar HTS Transwell-24, Falcon HTS Multiwell insert systems, and Millipore Multiscreen™ CaCo 96-well plate. The REMS AutoSampler is designed to facilitate integration with other robotic systems. Special locating bars are installed on the REMS base platform that allow other system robots to place an HTS tray into a precise location on the REMS base. The REMS AutoSampler automatically measures and records tissue resistance from a user-specified matrix of culture wells on the microplate. According to the specified sequence, the robotic arm moves over the identified wells taking TEER measurements. By means of a x-y-z locating system, the electrode-containing arm is positioned precisely and reproducibly over each well. The ability of the REMS AutoSampler to reproducibly and precisely locate the electrode results in highly reproducible TEER measurements. TEER measurements are stored in the computer as the electrode moves from one well to the next. The Windows-based software provides user-friendly features to acquire, display and store the tissue resistance measurements. The REMS electrode is compact and robust in design. Each of two rod-shaped probes, 1.5mm in diameter, consists of a pair of electrodes: one electrode for injecting current and the other for measuring the voltage. The use of two pairs of electrodes eliminates the error caused by the electrode-liquid interface. To take a measurement, the robot inserts one probe into the center of the filter well and the other into the opening slot of the 24- or 96-well plate. The use of AC current to measure resistance provides several advantages over DC current, including: Absence of offset voltages on measurements There is a zero net current being passed through the membrane, and therefore it is not adversely affected by a current charge No electrochemical deposition of electrode metal. The REMS AutoSampler also features a rinse and calibration check station. If occasional rinsing of the REMS electrode is required, it maybe sent to a ri
  6. TEER Research: WPI's Manual and Robotic Systems
    October 11, 2013
    Transepithelial Electrical Resistance TEER measurements is the most convenient, reliable and non-destructive method for evaluating and monitoring the growth of epithelial tissue cultures in vitro. The confluence of the cellular monolayer is quickly determined by a sharp increase in TEER. TEER measurement technology, which was first introduced by WPI in the mid-1980's, has since been perfected and expanded to include a range of TEER related manual and automatic instrumentation, including: EVOM² - Manual TEER measurement of epithelial cells in 24- and 96-well plates REMS AutoSampler - Automated system for High Throughput Screening (HTS) EVOM2 Volt-Ohm Meter
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