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  2. How to Choose the Correct NanoFil Tip Size for Microinjections
    February 25, 2021
    NanoFil™ is a unique low volume syringe developed for improved microinjection in mice and other small animals. During our field trials, the 35-gauge needle was scientists’ most popular and preferred NanoFil tip. The combination of its strength, length, durability and clogging resistance creates a solid balance. The 33-gauge tip is similar to Hamilton's 7762 and 7803 series removable needles in both tip length and outer diameter. However, our beveled tip version is shorter, more durable, and penetrates better due to the special tri-surface grinding technique. 33-gauge tips used to be the smallest size sold, but our 35-gauge tip is much better for injections involving small ani
  3. NanoFil Needles Cause Less Tissue Damage
    February 25, 2021
    NanoFil™ is a unique low volume syringe developed for improved microinjection in mice and other small animals. NanoFil needles are specially designed with a small outside diameter and spear point for shallow penetration and to cause the least amount of tissue damage.    Specially designed tips as small as 36 gauge (110 micron OD) are offered in both blunt and beveled styles. These tips will cause less trauma to the tissue than any other form of micro syringe currently in use. NanoFil can be used with many forms of small tubing and tips coupled with the syringe barrel. The blunt tip is used for injection into soft tissue a
  4. How to Setup the NanoFil System for Injections
    February 25, 2021
    NanoFil™ is a unique low volume syringe developed for improved microinjection in mice and other small animals. Here we show you several ways in which the NanoFil can be used for making injections. You can install the syringe on WPI’s UMP3 UltraMicroPump, using SilFlex tubing and a holder, so you can accurately inject by hand. You can install it in a stereotaxic frame. You can also directly inject by hand. NanoFil can be used in several different configurations: Install it on WPI’s UMP3 UltraMicroPump, which allows nanoliter resolution and reproducibility. For neural system injection, you can m
  5. NanoFil Microliter Syringes for Accurate Microinjections
    February 25, 2021
    NanoFil™ is a unique low volume syringe developed for improved microinjection in mice and other small animals. It comes in both 10 and 100 microliter volumes and can be paired with a UMP3 UltraMicroPump for making accurate microliter injections. NanoFil stainless steel needles range in size from 33 to 36 gauge. NanoFil™ is a unique low volume syringe developed for improved microinjection in mice and other small animals. It makes quantitative nanoliter injection much easier and more accurate than any other method. NANOFIL is a 10microliter syringe, and NANOFIL-100 is a 100 µL syringe. The large volu
  6. Why Buy a PUL-1000 Research Puller for Making Micropipettes?
    February 25, 2021
    PUL-1000 is a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes used in intracellular recording, patch clamp, microperfusion and microinjection. The PUL-1000 is capable of producing a vast array of pipette shapes, such as a long graduated taper, a short graduated taper and a short taper bee stingers. Here's a quick introduction the PUL-1000 research puller. The PUL-1000 puller was designed with tight mechanical specifications and precision electronics for complete control of the pulling process and accurate reproducibility. It offers programmable sequences of up to four steps with heating, force, movement and cooling time. A glass capillary is heated by a platinum/iridium filament and pulled by a controlled force. The PUL-1000 is capable of producing a vast array of pipette shapes, such as a long graduated taper, a short graduated taper and a short taper bee stingers. Programming The settings for a 4-stage pull may be stored in memory. Up to 95 programs can be permanently stored in memory. The instrument contains 15 factory installed programs. Choose from the factory installed programs for a quick start or create your own for precise, custom results.  Construction Tempered Glass Cover The cover of the pulling chamber is made with tempered glass to minimize the effects of humidity and air currents on the reproducibility of pulled pipettes.  Switching Power Supply
  7. DCAP - Humane Dispatch of Laboratory Rodents
    February 22, 2021
    DCAP guillotines are designed for small animal researchers, and they come in three sizes. The DCAP is considered one of the most humane methods of dispatching laboratory animals. With a single downward motion of the handle, you can euthanize a laboratory rodent quickly and without trauma.   These guillotines are designed for small animal researchers, and they come in three sizes. The guillotine is considered one of the most humane methods of dispatching laboratory animals. With a single downward motion of the handle, you can euthanize a rodent quickly and without trauma. The blades are comprised of hardened steel, so they hold an edge and remain sharp, offering years of service. Sharp blades make the process swift for both the researcher and the subject. With proper care, the stainless steel blades resists corrosion, too. A heavy-duty spring at the top of each guillotine securely holds the blade in an open position. This prevents the blade from accidentally falling and injuring laboratory personnel. It also makes it quicker to dispatch the subject, requiring only a single downward action. This guillotine was designed specifically for lab work. The large base affords greater stability. The long handle provides extra leverage. The spring action prevents the blades from falling unexpectedly. The hardened stainless blades ensure longevity of the unit. The simplified construction means easy maintenance. And the fluoropolymer-coated surface on the base makes cleaning easy. The DCAP guillotine is easy to use safely in your laboratory. If you have any questions, give us a call at (866) 606-1974 (Toll free in the USA) or email us at wpi@wpiinc.com.   See the DCAP  
  8. Five Factors That Affect the Pulling of Glass Micropipettes
    February 22, 2021
    Pulling micropipettes or microelectrodes is a science and an art, requiring some finesse. Here we discuss the five major factors which can affect the shape of a pulled glass micropipette or microelectrode. The PUL-1000 is a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes. Here we will look at factors that affect the pulling of glass.  As the temperature of glass increases, the glass transitions gradually from a hard and relatively brittle solid state into a soft and viscous state. To form glass into certain shapes using a puller, heat is applied through a filament. Many factors affect the heat transferred from a filament to a glass capillary. The filament holders heat up when current passes through the filament, which
  9. How to Adjust for Mechanical Drift in an M3301 Manual Micromanipulator
    February 12, 2021
    Over time, you may experience mechanical drift in one or more of the axes of your manual micromanipulator. Here we show you how to adjust for that. The process is similar for other styles of manual micromanipulators. We will look at the process for adjusting all three axes of a KITE micromanipulator.     A micromanipulator axis may begin to move gradually under its own weight even if it's not being touched. This phenomenon is referred to as mechanical drift, and it occurs from normal use of the manipulator axes over time. Each coarse axis control of the micromanipulator has its own separate adjustment to counteract drift. Let's look at the popular M3301 Micromanipulator. The
  10. How to Mount an Electrode Holder on Your Micromanipulator
    February 12, 2021
    The M3301 is a popular micromanipulator used for laboratory research. Here we will show you how to mount a microelectrode holder on the M3301 Micromanipulator. The procedure is very similar for most manual micromanipulators. In this video, we use the popular M3301 Micromanipulator to demonstrate how to mount a standard electrode holder. This M3301 is mounted on an M-3 Tilt Base which allows you to position it the way you want. The Tilt Base is mounted on a 5-lb. Weighted Base (WPI #
  11. How to Extend the Life of Your Micromanipulator
    February 12, 2021
    Manual micromanipulators are common equipment in a laboratory, and with proper care and handling they should last many years. Here's a few tips on how to take care of your new manipulator. Your micro manipulator is a precision instrument, and it has been calibrated at the factory and is ready to use. As with any delicate mechanical device, your care and attention ensure long-term accurate performance. The following are some helpful hints that make this possible. When handling a micromanipulator like the M3301 or the KITE, always set it down carefully. Dropping it even a short distance can damage the general alignment and the adjustment. When it's not in use, close all the slides. When the guides are exposed, dust can get in those tracks. When you're not using you manipulator, you want to close those up so the guide track are completely covered. Always cover the micromanipulator with a plastic bag or some kind of covering to keep the dust off. Dust particles and dirt are your biggest enemies. They get in the tracks and clogs the mechanisms. If the manipulator is not in use for a long period of time, be sure to occasionally work the slides to keep the grease pliable. Work them back and forth to keep them keep the grease pliable. Don't ever oil the guide surfaces. Under normal use, you shouldn't even need to apply grease for many years. If you have any questions, give us a call at (866) 606-1974 (Toll free in the USA) or email us at wpi@wpiinc.com.       See All Manual Manipulators  
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