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  1. Data Acquisition Designed for Muscle Physiology Pre-Clinical Trials
    October 20, 2015
    LabTrax 8/16 hardware combined with MDAC software is an excellent choice for WPI's SI-H line of muscle physiology products like the SI-MT Muscle Platform, SI-CTS Cell Tester and SI-HTB Horizontal Tissue Baths. This data acquisition system is ideal for testing physiological characteristics like the contractile and elastic properties of different tissues in various conditions like isometric, anisometric or isotonic states. This data acquisition system was designed with the muscle researcher in mind, because the knowledge of physiological characteristics of muscles is critical when quantifying the beneficial or adverse effects of a stimulus (like a pharmaceutical drug) on muscle function in pre-clinical studies.  Up to 8 Channels with Independent Control LabTrax 8/16 is a powerful low noise and high resolution data acquisition system, allowing up to 8 ADC channels and 4 DAC channels synchronously for user-defined muscle length and electrical stimulation protocols. It includes a number of adjustable standard functions like DC levels, ramps, square waves, sinus waves and staircases so you can define your own muscle length and electrical stimulation protocols. These standard functions can be combined in any configuration to create specific, user-defined, experimental protocols. In addition, two 5V TTL standard BNC outputs are available to drive further devices. Thanks to its global feature in defining numerous lengths change and electrical stimulation protocols, and its standard BNC connections, LabTrax 8/16 with MDAC can drive external devices.  Experimental Protocols in Muscle Physiology In muscle physiology, specific experimental protocols can be programmed to measure muscle contractile and elastic properties like twitch or tetanus protocols (for example, cardiac or skeletal muscle), quick stretch-relaxation protocols to calculate dynamic force or static force, or sinusoidal perturbation protocols (for example, lung tissue mechanics) to describe force-length loops to obtain dynamic stiffness and hysteresis effects of biological tissue. In addition, fatigue protocols may be individually programmed.   Continuous Stimulation for Cardiac Cells New features of LabTrax 8/16 with MDAC software include the use of continuous stimulation protocols to maintain especially cardiac cells during resting periods. Annotating the Data Annotation marks can be set during acquisition and read in the analysis options. Data Analysis Included with Data Acquisition Software MDAC software also includes a standard offline Analysis Toolbox for data processing and data analysis. Data processing and data analysis methods can be applied to each single channel individually. Software Filters for Data MDAC data processing allows for the use of different filter options to smooth the signal or to delete frequency components, using adjustable Butterworth low-pass, high-pass, band-pass or band-stop filters. MDAC includes the use of adjustable smoothing filters like Savitzky-Golay or Moving average. Down sampling options further smooth the signal and reduce the data amount. Mathematical and Statistical Calculations of Data MDAC data analysis allows for running mathematical operations on the acquired signals, like the calculation of the derivative or integral. In addition, the derivative can be calculated on the maximum or minimum value of the signal or on a value of a specific cursor position. Within these options, the calculation of a slope value for a given signal or the latency between two signals is possible. Finally, standard statistical analysis within MDAC can calculate the mean or median value for a selected interval and its corresponding standard deviation, as well as, the minimum and maximum value within the interval. For all data processing and data analysis methods, the raw data set can easily be restored during analysis, while already processed data can be stored as binary file. Further automated standard analysis, like twitch or tetanus, or customer defined analysis routines may be included as specific toolboxes. Excellent Data Acquisition System for Muscle Physiology Applications MDAC software combined with LabTrax 8/16 hardware is a reliable and affordable solution for muscle physiology applications.    More Info
  2. Recording TEER Measurements from an EVOM2
    November 11, 2014
    The EVOM2 is the classic, hand-held instrument for making Trans Epithelial Electrical Resistance (TEER) measurements. The REMS system adds a robot and data recording for automated TEER measurements. There may be times when you don't have a robot, but would still like to have the data recording capabilities of the REMS system. With a little ingenuity, you can do just that. Here, we will show you how you can add data recording to your standard EVOM2. Open Labscribe3 on your computer. Let the program identify the data recorder. In our example, we will use a Lab-Trax-4. Choose the default settings. Setup the preferences for one channel. If you like, you may add five points of smoothing.  In the marks section of the main window, select A1 (channel one). This records a data point directly into the marks records window. Alternatively, the smoothed graph C1 may be selected. In the top graph, right click and choose Units>Simple to calibrate the graph to ohms. Enter these values for the EVOM2 conversion: 2 point calibration Apply units to new data Value at Cursor 1: 0.0000 => 0.0000 Value at Cursor 2: 1.0000 => 1000 Unit Name = Ohms To connect the EVOM2 to the data recorder, connect a BNC cable from the Output BNC on the front of the EVOM2 to the Channel 1 input BNC on your data recorder. In the Marks window, enter A1 or Control. Move the electrode to the control well, and allow it to stabilize. Then, press Enter. Otherwise, it is difficult to hold the electrodes still while typing. For each additional well, enter in the number A2…, A3 into the Marks field. Then, move the electrode to that well and press the enter key after the reading stabilizes. Stop the recording and save the file. Open up the marks editor in Labscribe (View>Marks>Marks). The dialog box shows the time mark data and resistance values.   Press the Export button and choose “All Marks.” Give your export file a name. The export files is a text file that may be import into Microsoft Excel or another spreadsheet application.
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