Microinjectors

$2,900.00

Order code

VAR-MICRO-ePUMP

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Microinjectors For All Your Microinjection Applications

Designed to simplify intracellular injection and a variety of other microinjection tasks, the WPI introduces three new Microinjectors:

Type	MICRO-ePUMP	uPUMP	PV850
Regulated compensation and injection pressure
Pressure output: 0.3–87 PSI
Low volume tubing assembly
Inject using foot switch (included)
Inject using a TTL pulse from external computer or pulse generator (Cable to connect to pulse generator included)
Inject manually using touch screen interface
Integrated pressure source
2-Stage foot switch to activate voltage for injection and MICRO-ePORE^TM
Onboard MICRO-ePORE^TM Pinpoint Cell Penetrator for targeted microinjection and increased viability of injected embryos Audio continuity tone indicating active probe Injection counter to indicate total number of injections User-adjustable frequency and voltage through touch screen

New Generation of Pressure Injectors

For All Your Microinjection Needs

WPI's new generation pressure microinjectors are quickly replacing the very popular legacy line of pnuematic valve PicoPumps Pressure Injectors. The PV820 and PV830 have been a staple in the microinjection market for decades. The three new injectors facilitate any type of microinjecion that involves glass micropipettes.

MICRO-ePUMP - Microinjector with Integrated Cell Penetrator and Internal Pressure Source
μPUMP - Microinjector with Internal Pressure Source
PV850 - Microinjector for Use with External Pressure Source

One common microinjection method uses a glass micropipette to inject a liquid substance into a living cell or into the intercellular space, typically using an inverted microscope. The glass microinjection needle tip can vary in size from sub-micron to about 5-6µm in diameter. It will be larger if you are injecting stem cells into an embryo. The needles penetrate the cell membrane or even the nuclear envelope, in order to introduce a vector into a cell or nucleus. The vector could be a DNA molecule that carries foreign genetic material into the host cell where it replicates. By altering the embryo, microinjection can be used to genetically modify various animals like mice, fish (zebrafish, medaka, etc.), C. elegans, rats, rabbits, and even large domesitcated animals like cattle, sheep and pigs.

Benefits

Convenient internal pressure source (MICRO-ePUMP and uPUMP only)
Intuitive user-interface
Small footprint takes up very little bench space
Easy to navigate with touch screen and control knob
High precision for reproducible injections – microliters to picoliter
All inclusive system
Plug-and-play system to begin working as quickly as possible
Compensation pressure comparisson for accuracy

Applications

Explosive sensing with insect-based biorobots
Electron transfer kinetics on mono and multilayer graphene
Creation of transgenic animals
- Any nucleic acid can be introduced into any kind of cell
- No length limitation (100’s of kb’s of DNA) on the transfer of foreign genes
- Used to genetically modify animals like mice, fish, C. elegans, rats, rabbits and large domestic animals such as cattle, sheep, and pigs
Injecting substances into cells
- Nucleic acids
- Organelles
- Kinases
- Histochemical markers (such as horseradish peroxidase or fluorescent yellow)
- Proteins, antibodies
- Metabolites
- Ions
Microperfusion, which is the precise delivery of a small amount (pL to µL) of drug to a single cell or group of cells, such as a pharmacological drug test
Analysis of early development processes like gastrulation, organogenesis, etc.

All Your Microinjection Accessories

Let WPI be your one-stop shop for all your microinjection needs. From microscopes and manipulators to pre-pulled glass pipettes, WPI offers many options for your microinjection system.

Details

Options

Order code	Integrated Pressure Source	Integrated MICRO-ePORE
MICRO-ePUMP	Yes	Yes
uPUMP	Yes	No
PV850	No	No

Benefits

Convenient internal pressure source (MICRO-ePUMP and uPUMP only)
Intuitive user-interface
Small footprint takes up very little bench space
Easy to navigate with touch screen and control knob
High precision for reproducible injections – microliters to picoliter

Applications

Microinjection of diverse compounds and biomolecules – DNA, RNA, proteins
Pre- and post-implantation in embryos of various species – mice, rats, monkeys, bovine, pigs, zebrafish, etc.

MICRO-ePUMP

Microinjector with Integrated Cell Penetrator and Internal Pressure Source

MICRO-ePUMP

uPUMP

Microinjector with Internal Pressure Source

μPUMP

PV850

Microinjector with External Pressure Source (pressure source not included)

PV850

WPI Microinjectors use carefully regulated air pressure for injecting cells with fluid. Injected volumes range from picoliters to nanoliters. The port supplies positive pressure for high-pressure ejection. The pressure port maintains a low positive “compensation” pressure to the injecting pipette between injection pulses to prevent fluid uptake through capillary action.

Timing, injection pressure and compensation pressure are adjusted independently using the touch screen interface. Time intervals can range from 2 seconds down to 10 ms or less, depending on the injection pressure setting. The injection pressure interval is triggered by using a foot switch or a computer-controlled TTL pulse (MICRO-ePUMP uses only a foot switch).

The WPI Microinjectors are designed to inject very small quantities of fluids, such as drugs, into cells or small organelles. Pressure injection is an especially useful alternative to electroionophoresis, since it does not mandate the use of charged ions. Two different positive pressures may be applied–one for ejection at high pressure and a second, lower pressure to prevent back filling of the pipette by capillary action.

The WPI Microinjectors offers separate regulated compensation (back filling prevention) and ejection pres-sures with a precision timing circuit that switches from injection pressure to compensation pressure automati-cally.

WPI’s MICRO-ePORE^TM Pinpoint Cell Penetrator technology is embedded inside the MICRO-ePUMP. When the researcher enables the The MICRO-ePORE^TM, it delivers a highly localized voltage signal to a targeted injection site to facilitate penetration with minimal trauma. The researcher determines the amplitude and frequency of the signal that best suits the application. The signal originates in the control box, and it is transmitted through the electrode interface cable to the microelectrode holder. A silver wire is used to transmit the signal into the electrically conductive substance being injected. A reference electrode is used to place the media at 0.0 V potential with reference to the generated voltage.
The MICRO-ePUMP offers separate regulated compensation (back filling prevention) and injection pressures with a precision timing circuit that switches from injection pressure to compensation pressure automatically.

System components

What is included with the system	Qty	MICRO-ePUMP	uPUMP	PV850
Injector	1	Yes	Yes	Yes
2-Step Foot Switch	1	Yes	Yes	Yes
99164 MICRO-ePORE^TM Injection Assembly	1	Yes
99788 MICRO-ePUMP Ground Cable	1	Yes
AC/DC 24 V Power Adapter	1	Yes	Yes	Yes
Capillary kit includes		300744	300753	300753
75122-110 1.0 mm Pipette Gaskets (green)	4	Yes	Yes	Yes
75122-210 1.2 mm Pipette Gaskets (black)	4	Yes	Yes	Yes
75122-310 1.5 mm Pipette Gaskets (red)	4	Yes	Yes	Yes
75122-410 1.65 mm Pipette Gaskets (white)	4	Yes	Yes	Yes
802828 O-ring Bunna N-002	2	Yes	Yes	Yes
75123 Pipette Handle Assembly	1	Yes	Yes	Yes
75125 Pipette Holder	1	Yes	Yes	Yes
99862 Tubing Assembly	1	Yes	Yes	Yes
99865 Adapter Tube Assembly	1	Yes	Yes	Yes
CBL102 6’ Cable, 3.5 mm Mini Phone Plug to BNC	1	No	Yes	Yes
803130 Stereo Splitter Cable, 3.5 mm	1	No	Yes	Yes
Input kit for Picopumps includes	1			3116
0.25” NPT Fitting for Nitrogen Tank Regulator	1			Yes
10’ Hard Tubing	1			Yes
Vacuum Fitting	1			Yes