Tidas-E Base Series Photo Diode Array Spectrometer

Features

• Low noise detection
• Fiber optic design
• An additional light source (like the D4H or FO-6000) is required 

WPI’s Tidas series is a high end fiber-optic spectrometer module designed for low noise applications. The Tidas series outperforms conventional bench-based spectrophotometers and CCD-based spectrometer modules, when it comes to high precision fiber optic sampling. It relies on a monolithic optical bench made by Zeiss, which is optimized for fiber optic applications. Most cuvette-based standard spectrometers lose more than 90% of light through expensive prism decoupling. The Tidas series is designed for fiber optic sampling cells. Using suitable light sources and sample cells, spectral detection in the wavelength range of 190 to 1100 nm can be performed at noise levels < 0.04 mAU peak to peak.

Applications

The Tidas series is ideally suited for WPI’s fiber optic sampling equipment. High sensitivity detection systems for flow analysis can be assembled using WPI’s Liquid Waveguide Capillary Cells (LWCC) with effective pathlengths ranging from 50 to 500 cm. These setups are frequently used in fluid injection analysis systems for nutrient analysis (nitrite, nitrate, phosphate, iron) in oceanographic applications. Microliter sampling systems for UV/VIS applications can be assembled, using WPI’s V-Vette or DipTip™ dipping probes.  

Software

There are separate software packages for data collection and data analysis for the Tidas-E. An instrument driver, TIDASDAQ, is used to run the spectrometer module, collect spectra in either single or continuous mode, control the digital I/Os and save the experimental data to disk. Data analysis is performed with the SpectraView software package. Further, TIDASDAQ exports data directly into GRAMS/AI, a feature very useful for advanced data analysis for pharmaceutical applications and requirements.

TidasDAQ 3 Labor: Data Sampling and Instrument Control

With TidasDAQ 3 Labor, high precision intensity, absorbance, transmittance or normalized spectra can be obtained in less than a second. Only a few parameters need to be adjusted to obtain spectral data. Sampling of single scans, continuous full spectra scans or triggered scans is possible. Chromatograms can be displayed and logged to disk at up to four wavelengths. Data Export of 2D and 3D Spectrograms, as well as Chromatograms is supported in ASCII, Spectralys/SpectraView, Excel and Grams/AI formats. Light sources and other sampling instrumentation can be controlled via the TTL level digital outputs, as well as data collection can be triggered by TTL leveled external inputs of the Tidas series.

TIDASDAQ 3 Labor acquisition window, showing an absorbance spectrum. Click on the image for a bigger picture. Monitor mode. (a) Button bar, (b) Graph area, (c) Parameter area and (d) Status bar.

TIDASDAQ 3 Labor: Data Analysis

TIDASDAQ 3 Labor software now includes a spectroscopy data analysis package targeted at flow analysis applications. It processes data recorded by TIDASDAQ 3 Labor. The Analysis module allows you to display recorded spectra in 2D (default) and 3D view. Mathematical computation, Derivation, Smoothing, Quantification and other functions are available to work with your data. The Analysis module allows for single point and multiple point analysis, multiple linear regression, partial least square and principle component analysis. Data can be exported out of a 3D analysis file into separate scans. Further, chromatograms as well as spectrograms can be copied directly into Excel for further data analysis. 

Main panel with color graphs. (a) Spectrum graph, (b) CM CIELab graph (same as CM CIELuv and CM CIELab (Hunter) types), (c) CM CIE graph and (d) CM Table. Graphs are set through setup window.

DNA/RNA Quantification with:
 • 2mm cuvette
 • DipTipMini