ISO-NOPF Flexible Nitric Oxide Sensor

Order code

Unique flexible NO sensor! Designed for arteries, microvessels, in vivo applications and similar applications

   Don't forget the SNAP!
  • Excellent selectivity to NO
  • Rapid response time
  • Highly sensitive
  • For use with TBR4100 and TBR1025
  • Requires cable 91580 (sold separate)
  • Lowest Detection Limit: 0.2 nM
  • Choose diameter, length and shape
  • Package
    • ISO-NOPF200 package of 2
    • ISO-NOPF100 package of 2
    • ISO-NOPF500-CXX package of 2
    • ISO-NOPF200-L10 package of 3

Note : It takes 3 days to test prior to shipping.


Order code Tip Length Tip Diameter Shape Package of
ISO-NOPF100 1-5 mm 100 µm Straight 2
ISO-NOPF200 1-5 mm 200 µm  Straight  2
ISO-NOPF200-L10  10 mm  200 µm L-shaped  3
ISO-NOPF500-CXX 5-10 mm  500 µm Straight 2
ISO-NOPF100-Lxx 1-10 mm  100 µm L-Shape 2
ISO-NOPF200-Lxx 1-10 mm 200 µm L-Shape 2


More Nitric Oxide sensor  


  • Flexible sensor, nearly unbreakable


  • In vivo nitric oxide measurement  

Flexible nitric oxide sensor

ISO-NOPF electrodes are available in 100 µm, 200 µm and 500 µm diameters. Utilizing the latest advances in nano-technology and material science, scientists at WPI’s Sensor Laboratory have created these completely flexible and virtually unbreakable NO sensors. The new sensors are based on a composite graphite NO-sensing element combined with a reference electrode. The surface of the sensor is then coated with a unique multi-layered NO-selective membrane.

Unique, flexible NO sensorInvivo graph

Designed for arteries, microvessels, in vivo applications, and similar applications. The graph (right) shows the response of the ISO-NOPF to NO.


These sensors are based on a composite graphite NO-sensing element combined with a reference electrode. The surface of the sensor is then coated with a unique multi-layered NO-selective membrane.

Selectivity of WPI's NO sensors

The ideal NO sensor should be insensitive to other reactive species likely to be present within the measurement environment. Conventional Nafion coated carbon fiber NO sensor exhibits a large response to such species. WPI's unique NO sensor technology utilizes an novel surface membrane which amplifies the response to NO whilst eliminating responses to a vast range of reactive species, including nitrite, ascorbic acid, hydrogen peroxide, catecolamines, and much more.

NOTE: ISO-NOPF200 is a 5mm long sensor, custom lengths available (1, 2, 3, 4 mm). When ordering custom lengths, use the part number ISO-NOPF200-CXX and replace the XX with the desired length. For example, if you want a 1 mm flexible sensor tip, the part number should be ISO-NOPF200-C01. This sensor can be ordered in the following custom lengths: 1 mm, 2 mm, 3 mm or 4 mm. Make your selection from the dropdown list before you place your order.

The ISO-NOPF500 is a nitric oxide sensor designed like the dry, carbon fiber ISO-NOPF sensors, however, it works like a traditional ISO-NOP 2mm sensor. The sensor can be ordered in a variety of lengths from 5-10mm. It incorporates WPI's propiretary combination electrode technology in which the nitric oxide sensing element and separate reference electrode are encased within a single sheilded sensor design. 

The ISO-NOP was the original nitric oxide sensor, ideal for cell cultures, cell suspensions and many other applications. The new ISO-NOPF500 can be used in the same way, but offers several advantages:

  • Requires no sleeves or filling solutions
  • Flexible and durable, like other ISO-NOPF sensors
  • High sensitivity for a rapid response time–ten times more sensitive than the ISO-NOP
  • Can be used in acidic conditions
  • Longer sensor tip than the ISO-NOP
  • Bigger linear range than the ISO-NOP (range is based on the length of the sensor tip)
  • Calibration with either the SNAP or Nitrite method

NOTE: When ordering custom lengths, use the part number ISO-NOPF500-CXX and replace the XX with the desired length. For example, if you want a 10mm flexible sensor tip, the part number should be ISO-NOPF500-C10. This sensor can be ordered in the following custom lengths: 5mm, 6mm, 7mm, 8mm, 9mm or 10mm. 

L-shaped Sensor

L-Shaped nitric oxide sensor

The ISO-NOPF200-L10 is a unique L-shaped nitric oxide sensor designed specifically for use in tissue bath studies and similar applications. The shape of the sensor has been engineered to facilitate placement of the electrode within the lumen of the tissue vessel under study. The ISO-NOPF200-L10 has a flexible tip (200 µm diameter).


Outside Diameter 100 μm 200 μm 200 µm 500 μm
Available Length 1-5 mm (sensor length varies in 1 mm
increments - 1 mm, 2 mm, 3 mm...)
1-5 mm (sensor length varies in 1 mm
increments - 1 mm, 2 mm, 3 mm...)
10 mm 5-10mm (sensor length varies in 1 mm 
increments - 1 mm, 2 mm, 3 mm...)
Response Time   < 5 seconds < 5 seconds < 10 seconds
Lowest Detection Limit/Range 0.2 nM  0.2 nM 0.2 nM 0.2 nM
Nominal Sensitivity-New sensor ≥10 pA/nM  ≥20 pA/nM ≥50 pA/nM ≥20 pA/nM
Baseline Drift none  none none none
Poise Voltage 865 mV  865 mV 865 mV 865 mV
Typical Quiescent Baseline Current, 25°C  2000 pA 2500 pA 3500 pA 5000 pA
Acceptable Baseline Range 500-8000 pA  500-8000 pA 500-8000 pA 3000-25000 pA
Polarization Time 2+ hours  2+ hours 8+ hours 8+ hours


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Liu, S., Gu, T., Fu, J., Li, X., Chronakis, I. S., & Ge, M. (2014). Quantum dots-hyperbranched polyether hybrid nanospheres towards delivery and real-time detection of nitric oxide. Materials Science and Engineering: C, 45, 37–44.

Araújo, F. A., Rocha, M. A., Capettini, L. S. A., Campos, P. P., Ferreira, M. A. N. D., Lemos, V. S., & Andrade, S. P. (2013). 3-Hydroxy-3-methylglutaryl coenzyme A reductase inhibitor (fluvastatin) decreases inflammatory angiogenesis in mice. APMIS, 121(5), 422–430.

Diniz, T., Pereira, A., Capettini, L., Santos, M., Nagem, T., Lemos, V., & Cortes, S. (2013). Mechanism of the Vasodilator Effect of Mono-oxygenated Xanthones: A Structure-Activity Relationship Study. Planta Medica, 79(16), 1495–1500.

Raithel, M., Hagel, A. F., Zopf, Y., Bijlsma, P. B., de Rossi, T. M., Gabriel, S., … Konturek, P. C. (2012). Analysis of immediate ex vivo release of nitric oxide from human colonic mucosa in gastrointestinally mediated allergy, inflammatory bowel disease and controls. Journal of Physiology and Pharmacology : An Official Journal of the Polish Physiological Society, 63(4), 317–325. Retrieved from

Araújo, F. A., Rocha, M. A., Ferreira, M. A., Campos, P. P., Capettini, L. S., Lemos, V. S., & Andrade, S. P. (2011). Implant-induced intraperitoneal inflammatory angiogenesis is attenuated by fluvastatin. Clinical and Experimental Pharmacology and Physiology, 38(4), 262–268.

Tang, X., Chen, J., Wang, W.-H., Liu, T.-W., Zhang, J., Gao, Y.-H., … Zheng, H.-L. (2011). The changes of nitric oxide production during the growth of Microcystis aerugrinosa. Environmental Pollution, 159(12), 3784–3792.

Andrews, A. M., Jaron, D., Buerk, D. G., Kirby, P. L., & Barbee, K. A. (2010). Direct, real-time measurement of shear stress-induced nitric oxide produced from endothelial cells in vitro. Nitric Oxide, 23(4), 335–342.

Mantione, K. J., & Stefano, G. B. (2004). A sub-nanomolar real-time nitric oxide probe: in vivo nitric oxide release in heart. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 10(4), MT47-9. Retrieved from


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