Nitric Oxide Sensor - 2mm
- 2 mm NO sensors for NO detection in cell cultures
- Cell Culture, cell suspension
The ISO-NOP is a popular, robust and high performance sensor encased within a 2 mm diameter disposable stainless steel protective sleeve. The tip of the sleeve is covered with a NO-selective membrane. Replacement membrane sleeves can be purchased separately (WPI #5436) and require an internal electrolyte (WPI #7325).
A simple change in experimental protocol enables the ISO-NOP to be conveniently used for indirect rapid accurate determination of nitrite (NO–2) and nitrate (NO–3) concentration in samples. Using this method a detection limit for NO–2 or NO–3 as low as 1 nM is routinely possible.
NOTE: The 2 mm biosensors come with a spare membrane sleeve, 1cc syringe, an MF28G67 MicroFil needle and 10mL of filling solution.
The image (right) shows an abdominal x-ray of an apparatus consisting of two customized ISO-NOP nitric oxide probes, 4-channel pH catheter and Teflon nasogastic tube. (Courtesy Prof. K.E.L. McColl, University Department of Medicine and Therapeutics, Western Infirmary, Glasgow, Scotland., lijima, K., et al. Gastroenterology 2003:122:1248-1257)
Selectivity of WPI's NO sensors
The ideal NO sensor should be insensitive to other reactive species likely to be present within the measurement environment. Conventional Nafion coated carbon fiber NO sensor exhibits a large response to such species. WPI's unique NO sensor technology utilizes an novel surface membrane which amplifies the response to NO while eliminating responses to a vast range of reactive species, including nitrite, absorbic acid, hydrogen peroxide, catecolamines and much more.
|Outside Diameter||2 mm|
|Response Time||< 5 s|
|Lowest Detection Limit/Range||1 nM|
|Upper Detection Limit/Range||80 µM (beyond 80 µM, sensor accuracy is not guaranteed)|
|Nominal Sensitivity (New sensor)||≤2 pA/nM|
|Interferent (Selectivity Coefficient)||NaNO2 (10-6 or better)|
|Poise Voltage||865 mV|
|Typical Quiescent Baseline Current, 25°C||3,000 pA|
|Acceptable Baseline Range||1,000-8,000 pA|
|Polarization Time||12+ hours|
|Recommended Polarization Solution||0.1 M KI/H2SO4|
J.E. Doeller, T. S. Isbell, G. Benavides, J. Koenitzer, H.Patel, R. P. Patel, J. R. Lancaster, Jr. V. M. Darley-Usmar, D.W. Kraus "Polarogaphic measurement of hydrogen sulfide production and consumption by mammalian tissues" Analytical Biochemistry 341. 2005: 40-51
Murine strain differences in inflammatory angiogenesis of internal wound in diabetes. (2017). Biomedicine & Pharmacotherapy, 86, 715–724. http://doi.org/10.1016/J.BIOPHA.2016.11.146
- ISO-NO Electrolyte, CO2-insensitive (10mL)7521$49.00
- T-Adapter Flow-Through Kit5399$53.00
- ISO-NOP Filling Solution7325$53.00
- Nitrite Standard Solution, 1g/L (100mL)7357$54.00
- Replacement sleeves for ISO-NO5436$201.00
- Universal Dipping Probe Stand42200$245.00
- Enzol Enzymatic Detergent, 1 Gallon7363-4$107.00
- Pre-polarizer for Nitric Oxide SensorsNSA-3$338.00