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Benefits

• PermaCell 96-well insert plates are advanced, sterile cell culture devices used for growth and differentiation of cells

• Plates consist of an array of 96 membrane wells connected in a single, rigid tray for easier handling and feeding and to allow for high-throughput robotic processing

• 96-well insert plate fits within a 96-well receiver plate (included) which allows for individual treatment and assaying of each well in the plate (right)

• PermaCell insert plates can be used for cell culture and cell differentiation into 2D/3D tissues, drug transport/permeability studies, and are easily adaptable to TEER measurements and other analytical techniques

• 96-well insert plates connect in a single, rigid tray

• Pore size of 0.4 µm

• PET membrane with high pore density 

• Large access ports for efficient media sampling

• Robotics and TEER compatible

• Large access ports to allow for efficient media sampling

• Each unit includes the 96-well insert plate, 96-well reservoir plate, and lid

NOTE: WPI is an authorized distributor of MatTek for the 96-well cell culture insert plates.

Plate Setup

It is best to perform these steps in a laminar flow hood or equivalent controlled environment. The inserts are pre-packaged in a 96-well receiver plate. Be sure to inspect the wells to ensure there are no defects before you begin.

1. Add cell culture media to the wells of the 96-well receiver plate. For MatTek 96-well insert plates, add 400 µL of medium into the wells of the receiver plate.

2. Seed cells into the wells of the insert plate and follow the standard tissue culture incubation and feeding procedures for optimal cell growth. Typical experiments seed cells on the apical membrane using up to 100 µL in the apical compartment. Experiments may also include seeding cells into the receiver plate wells and/or onto the basal side of the membrane.

NOTE: For TEER measurements, use the following media or buffer volumes.

Once insert plates are opened, plates cannot be returned for a refund. 

WPI is an authorized distributor of MatTek PermaCell insert plates.