In epithelial cell research, there are different types of cells which are typically cultured. We will focus on epithelial cells. Epithelial tissue lines the surface and cavities of an organ. Epithelium includes your skin tissue, but it also lines the alimentary canal, organs and blood vessels. Epithelial cell cultures may be used for drug discovery or other applications that explore the transport or diffusion of a substance through an epithelial monolayer. Such a tissue research study requires a confluent monolayer that completely covers the culture substrate it is grown on. WPI pioneered the production of TEER measurement equipment in the 1980s and is still innovating new equipment for cell researchers.
TEER Measurement Uses for In Vitro Research
For several decades, transepithelial electrical resistance (TEER) measurement has become universally established as the most convenient, reliable and non-destructive method of evaluating and monitoring the growth of epithelial tissue culture in vitro. The confluence of the cellular monolayer is quickly determined by monitoring TEER. There is a gradual rise in TEER measurement as the gaps in the monolayer close. At confluence, the resistance plateaus and quits climbing. TEER measurement technology, first introduced to tissue and cell biologists by WPI in the mid 1980s has since been perfected and expanded to include a range of TEER related manual and automatic instrumentation.
Manual TEER Measurement
We offer both manual and automatic systems for making TEER measurements. The EVOM2 is the epithelial volt/ohm meter routinely used by tissue biology researchers to manually monitor the health of cellular monolayers. The EVOM2 comes with the chopstick style STX2 electrodes. For more repeatability, the STX100 series of electrodes are recommended. The EndOhm chambers were designed for endothelial measurements.
Automatic TEER Measurement System
Routine drug discovery studies, clinical trials and other cell research often requires a high throughput screening system for TEER measurements. The REMS automated robotic system is an excellent and affordable choice. The REMS works with 24 and 96 well cell culture plates from a variety of manufacturers.