WPI Products for Microinjection

Microinjection uses either a metal needle or a glass micropipette to inject small liquid volumes. For example, genetic material may be inserted into a living cell, a drug introduced into an eye or brain, or fluid injected into a muscle. Typically, microinjection is performed under a microscope. A stereotaxic setup may be required.

WPI offers a variety of pumps along with special syringes, stereotaxic frames, glass capillaries and needles. Here we will highlight our microinjection pumps. The setup you choose depends on the size of injection aliquots, the volume to be injected and the size of needle or glass tip chosen. The products described below are listed in order by the smallest volume they can inject. See the comparison chart below or the related post links at the bottom.

Pressure Injectors

WPI's new generation of pressure microinjectors are quickly replacing the very popular legacy line of pnuematic valve PicoPumps Pressure Injectors. The three new injectors facilitate any type of microinjecion that involves glass micropipettes.

• MICRO-ePUMP - Microinjector with Integrated Cell Penetrator and Internal Pressure Source
• μPUMP - Microinjector with Internal Pressure Source
• PV850 - Microinjector for Use with External Pressure Source 

PV830, the legacy Pneumatic PicoPump, was designed to simplify intracellular injection. You get repeatable microinjection in volumes ranging from picoliters to nanoliters. PV830 offers eject and hold pressure. The hold pressure prevents backfilling of the pipette by capillary action. In addition, the PV830 also has vacuum pressure which allows you to securely hold a cell with one pipette while you inject it with another. The volume injected is controlled by the inside diameter of the glass tip, the pressure and the time. This pump can inject fluids in the picoliter to microliter ranges.

UMP3

UMP3 was the first micro syringe pump on the market, and design improvements ensure it remains the best choice for stereotaxic brain injection applications. It is a motorized syringe pump that accepts syringes from 0.5µL–1mL. Using a 10µL syringe, the actual practical minimum volume is 25nl. For intuitive, intelligent control, the UMP3 is combined with the Micro2T SmarTouch controller. It is built with extremely tight tolerance for high accuracy. It can be attached to the M3301 manual micropositioner or any manual stereotaxic manipulator.

Nanoliter 2020

NANOLITER2020 is the updated version of WPI’s popular NANOLITER2010 Injector that is capable of performing precision injections in the nanoliter range using a variety of glass micropipette sizes. The new model connects directly to the WPI MICRO2T SMARTouch™ controller, which can also be used with WPI’s UMP3 pump, allowing the pumps to be controlled individually or synchronously. The new injector head has a LED indicator which shows proper communication between the pump and controller. NANOLITER2020 offers improved precision by in-depth plunger displacement validation. The system is easier to use and has a chic silver injector head. The NANOLITER2020 system is perfect for oocyte injection and applications involving injections as small as 6nL. It offers fast, accurate aliquot injections. It works with glass pipettes. It is back filled with oil, the oil is nearly pressed out, and then it is front filled with the injection fluid. The maximum volume is limited by what the glass pipette can hold.

Manual Microsyringe Pumps

The MMP and DMP systems are manual microsyringe pumps. They use a manual micrometer to eject the fluid. The DMP micrometer has a digital display. Both use glass micropipettes. These are simple, hand operated systems for perfusion or withdraw of fluids.

Accessories for Microinjection

Comparing WPI Pumps

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