Nanoliter 2020 Injector
Precise nanoliter-volume injections with intuitive SMARTouch™ controller
NANOLITER2020 is the complete system. 300704 is the NANOLITER2020 Injector Head only, and the controller and the accessories are not included.
- Perform nanoliter volume injections using glass micropipettes
- Nanoliter Injector works on the principle of Positive Displacement. An internal micrometer step motor precisely moves the metal plunger which pushes the oil inside the micropipette, and the oil-layer pushes nanoliter volumes of aqueous sample out of the pipette tip.
- Fine control of plunger displacement along with proper sealing among gasket, glass micropipette, and oil ensures precision and accuracy
- NANOLITER2020 System includes the 300704 NANOLITER Injector, the MICRO2T SMARTouch™ Controller and many necessary accessories (See Details)
- MICRO2T SMARTouch™ controller is required to operate the 300704 Injector (The same MICRO2T controller can be used with UMP3 pumps.)
- 300746 Spare Parts Kit required for use with the 300704 Injector
- Glass micropipettes are not included with the 300704. Use WPI glass micropipettes or use glass capillaries (504949 or 504950) and a puller to make micropipettes.
- Use a tweezer (501981) to scoop gaskets out of the NANOLITER2020 Injector Head
- 300704 Injector Head comes with one set of gaskets installed in the injector to use with 1.1-1.15 mm OD fire-polished glass
|NANOLITER2020||Complete system (Injector and Controller)|
|300704||NANOLITER2020 Injector Head only|
|MICRO2T||SMARTouch Controller only, Two-Channel|
Benefits of NANOLITER2020 Injector (300704)
- Injections performed with mineral oil back-filled glass micropipettes, and the sample is front filled. This approach minimizes loss of any costly and scarce sample when a minute sample volume can be adequate. (See FAQ for details.)
- Control up to two pumps simultaneously
- Has capability to be easily mounted on a micromanipulator (e.g., M3301) or a stereotaxic frame (e.g. 505313) using the universal adapter 500778 (included)
- Precise control over injection volumes (in the nanoliter to microliter range) and injection rates
- Simple to use touchscreen interface and graphical representation of volume status on MICRO2T controller
- LED run indicator offers a visual indication of connectivity
- Perform the injection with a foot switch (optional)
- Injector is compatible with different OD glass micropipettes (in the range of 1.1-1.5 mm)
- NANOLITER2020 costs 15% less than the previous model (NL2010MC2T)!
Nanoliter to microliter injection into frogs (Xenopus oocytes), rats, mice, mosquitoes, shrimps, insects (e.g., Brown Planthopper), flies (e.g., Drosophila) and fish (Zebrafish) embryo using glass micropipettes
Please refer to the “References” tab for details.
- Connects directly to our MICRO2T SMARTouch™ controller, which can also be used with WPI’s UMP3 pump
- The injector head has a LED indicator which lights up bright red in RUN mode and dim red when connected to the controller. The LED indicator shows proper communication between the pump and controller.
- 1.14 mm OD fire polished glass micropipette is recommended for use with front green gasket. Use different OD fire-polished glass micropipettes by switching the front gasket (Green gasket for 1.1-1.15 mm OD glass, Black for 1.3-1.35 mm, Red for 1.5 mm).
- Improved precision by in-depth plunger displacement validation
- System is easier to use or operate
- Injector has a chic silver design
NANOLITER2020 System includes all the components listed below.
NOTE: The 300704 Injector may also be purchase separately. 300704 does not include the Controller or any of the required accessories listed in the table below.
NANOLITER2020 Injector Head with Universal Adapter 500778[One Set of Gasket (Front Green Gasket) to use with 1.1-1.15 mm Glass is installed]
|Items listed below are already included with NANOLITER2020 System or can be purchased separately:|
|501981||Tweezers to scoop out gaskets||1|
|504949||Glass Capillary (ID = 0.530 mm, OD 1.14 mm)||1 (pack of 300)|
|1TIP10XV119||Glass Micropipettes (ID = 0.530 mm, OD 1.14 mm, Tip ID= 10 μm)||2|
|300746 Spare Parts Kit includes:|
|14456 Allen Wrench 0.035 Hex Tool||1|
|MF34G MicroFil 34 ga||1|
|300733 O-Ring Kit for NANOLITER2020||5|
|3563 1 cc Syringe||1|
|300514 Replacement Piston for NANOLITER2020||2|
NANOLITER2010 vs NANOLITER2020
|Type||NANOLITER 2010 Injector||NANOLITER 2020 Injector|
|Compatibility with 1.14 mm OD Fire-polished Glass Micropipettes||Yes||Yes|
|Compatibility with 1.3- or 1.5-mm OD Fire-polished Glass Micropipettes||No||Yes|
|Mount (500778) included to attach onto a micromanipulator or stereotaxic frame||Yes||Yes|
|Compatible with Standard Controller||Yes||No|
|Compatible with MICRO2T and MICRO4 Controller||Yes||Yes|
|Adapter to connect to MICRO2T is Required||Yes||No|
|LED Indicator is Present||No||Yes|
|Foot Peddle Controllable||Yes||Yes|
Frequently Asked Questions (FAQ)
- What is the advantage of using oil back-filled glass micropipettes?
The term “back filling” refers to the process of filling the pipette from the large, non-pulled end. “Front filling” is the term which is used to describe filling a micropipette through the small, pulled front end of the pipette. The glass micropipettes are first backfilled completely with mineral oil and secured to the NANOLITER Injector Head. Then, some mineral oil is dispensed through the tip. This creates the space and generates the pressure to front-fill samples through the tip. Front filling sample prevents the spillage or loss of costly or scarce sample involved with back-filling samples. When the sample volume is low, first back-filling glass micropipettes with oil and then front-filling the sample may be the only option.
- Can I use WPI’s old standard controller to run the 300704 Injector head?
WPI NANOLITER2020 Injector Head (300704) needs the MICRO2T controller (recommended). WPI’s MICRO4 controller may be used to control the pump. The 300704 Injector Head cannot be used with WPI’s old standard controller.
- Can I use same MICRO2T controller that I use with UMP3 pump? Do I need an additional adapter to use the 300704 pump with my existing MICRO2T controller?
Yes, the same MICRO2T controller can be used to control the UMP3 pump and the Nanoliter2010. No additional adapter is needed. 300704 connects directly to MICRO2T controller.
- Can I run the pump using a footswitch?
Yes, you can use the footswitch 13142. This is not included in the NANOLITER2020 system and is sold separately.
- What do I do when glass micropipettes come off after attaching or I am unable to front-fill a sample?
Odorless and colorless mineral oil is used for backfilling glass micropipettes. While the glass micropipette is installed and with gradual spillage over multiple uses, the gaskets become too slippery to hold glass micropipette in place. Use Kimwipes to soak up oil spillage inside the injector head, outside the glass micropipettes and to clean the gaskets. Wipes sprayed with 70% ethanol or isopropanol can be used for cleaning. If cleaning the oil spillage did not solve the issue, a new set of gaskets needs to be installed.
- I position the O-rings in the right order and orientation per your video but still don’t get an air tight seal .What can be done?
If the rear seal is loose or mounted backwards then it can leak from air passage. This gasket needs to be tight on the plunger wire. If the pipette is pull tested, then it can be easily unseated from the middle gasket and leak by air infusion. The butt end of the glass needs to be smooth (and without any cracks in the glass) and firmly held down onto the middle seat while the collet is being screwed down. That plastic seat will deform a small amount and "mold" itself to the end of the glass. The fluids can also flow out on their own without any added back pressure even if there is a good seal, but that amount of mixing at a 10 um tip is very small. If either rear gasket allows for air flow, then it will leak.
NANOLITER2020/300704 Injector Specifications
|NANOLITER 2020 Plunger Outer Diameter||482 µm|
|Plunger Movement for 100 nL Volume Dispense||550 µm ± 55 µm|
|Piston Movement per dispensed volume (nL)||5.5 µm/nL|
|Linear Travel Per Full Step||12.7 µm/step|
|Glass Use Capabilities||
|Minimum Injection Volume||6 nL|
|Recommended Minimum Injection Volume||
|Maximum Possible Volume||4200 nL|
|Maximum Rate||644 nL/sec|
WPI's previous versions of NANOLITER2020 (NANOLITER2010, NL2010MC2T, and NANOLITER2000) that worked on same oil-plunger based, positive-displacement technique were used for research studies involving injection into rats, mice, mosquitoes, shrimps, frogs (Xenopus oocytes), insects (e.g., Brown Planthopper), flies (e.g., Drosophila), and fish (Zebrafish) embryo.
Gaete, P., Lillo, M., López, W., Liu, Y., Harris, A., & Contreras, J. (2020). A novel voltage clamp/dye uptake assay reveals saturable transport of molecules through CALHM1 and connexin channels. BioRxiv, 2020.02.15.950923. https://doi.org/10.1101/2020.02.15.950923
Atif, M., Lynch, J. W., & Keramidas, A. (2020). The effects of insecticides on two splice variants of the glutamate‐gated chloride channel receptor of the major malaria vector, Anopheles gambiae. British Journal of Pharmacology, 177(1), 175–187. https://doi.org/10.1111/bph.14855
Hao, D.-L., Yang, S.-Y., Liu, S.-X., Zhou, J.-Y., Huang, Y.-N., Véry, A.-A., … Su, Y.-H. (2020). Functional Characterization of the Arabidopsis Ammonium Transporter AtAMT1;3 With the Emphasis on Structural Determinants of Substrate Binding and Permeation Properties. Frontiers in Plant Science, 11, 571. https://doi.org/10.3389/fpls.2020.00571
Li, Y., Liu, Z., Guo, Q., & Luo, M. (2019). Long-term Fiber Photometry for Neuroscience Studies. Neuroscience Bulletin, 35(3), 425–433. https://doi.org/10.1007/s12264-019-00379-4
Li, R., Weng, J., Wang, X., Meng, Q., Wang, Y., & Sun, J. (2019). Bursicon homodimers induce innate immune by activating the expression of anti-microbial peptide genes in the shrimp Neocaridina heteropoda. Fish and Shellfish Immunology, 84, 906–911. https://doi.org/10.1016/j.fsi.2018.10.080
Li, R., Huang, Y., Zhang, Q., Zhou, H., Jin, P., & Ma, F. (2019). The miR-317 functions as a negative regulator of Toll immune response and influences Drosophila survival. Developmental and Comparative Immunology, 95, 19–27. https://doi.org/10.1016/j.dci.2019.01.012
Li, X., Liu, F., Wu, C., Zhao, J., Cai, W., & Hua, H. (2019). Decapentaplegic function in wing vein development and wing morph transformation in brown planthopper, Nilaparvata lugens. Developmental Biology, 449(2), 143–150. https://doi.org/10.1016/j.ydbio.2019.02.016
Scala, R., Maqoud, F., Angelelli, M., Latorre, R., Perrone, M., Scilimati, A., & Tricarico, D. (2019). Zoledronic Acid Modulation of TRPV1 Channel Currents in Osteoblast Cell Line and Native Rat and Mouse Bone Marrow-Derived Osteoblasts: Cell Proliferation and Mineralization Effect. Cancers, 11(2), 206. https://doi.org/10.3390/cancers11020206
- Micropipette Tips for Nanoliter 2020/2010TIP10XV119$175.00
- Glass Capillaries for Nanoliter 2020/2010, fire polished504950$75.00
- Optional Foot Switch for Micro2T, Micro-ePore™, DMF100013142$152.00
- Economy Tweezers #7Multiple SKUs
- Plunger for Nanoliter 2020/2010/2000500299$58.00
Using WPI's New NANOLITER2020 Injector
Setting Up Your Nanoliter 2020
Front Filling a Micropipette with the WPI Nanoliter2010 Injector
Nanoliter2010 Replace Gasket
Setting Nanoliter2010 Speed and Volume