Low Toxicity Silicone Adhesive
Ideal for neuroscience applications, nerve studies and more
- Bio-compatible surgical adhesive for live tissue and nerve studies
- Pre-mixing tips simplify use (Includes 10 mixing tips)
- Medium strength adhesion
- Low toxicity
- Rapid curing silicone adhesive, cure on contact
- Cures without producing heat
- The kit comes with (2) cartridges of 2 part silicone
- Kwik-Sil surgical glue dries clear; Kwik-Cast dries green
- Kwik-Sil surgical glue dries a little faster than Kwik-Cast
- Low toxicity surgical adhesive
- Rapid cure time
- Neuroscience and nerve studies
- Biomedical applications
Kwik-Sil and Kwik-Cast silicone adhesives have very low toxicity before, during and after curing. The by-product of curing is a small amount of hydrogen gas, which is much less toxic to cells than acetic acid or alcohol from traditional RTV silicone systems.
Kwik-Sil and Kwik-Cast curing speed is hundreds of times faster than traditional RTV silicones. A curing time of a few minutes at room temperature is especially useful for encapsulation of live tissue or implanting into a live animal. Unlike many vinyl-based silicones in which the platinum complex catalysts are easily poisoned by contamination from amines and animal tissue, Kwik-Sil and Kwik-Cast surgical adhesives are not sensitive to contamination from animal tissue.
Kwik-Sil™ is a translucent, medium-viscosity silicone adhesive, developed for chronic peripheral nerve studies such as anterograde tracing with fluorescent indicators or electrode recording. Good adhesion and mechanical properties (tear strength and elongation) allow days of study without breaking of the bonding. Curing speed is very reproducible.
Kwik-Cast™ is a very low viscosity silicone sealant developed to embed peripheral nerves with electrodes for acute multi-fiber recordings. It flows easily, filling the small spaces around the nerve and leaving no channels through which peritoneal fluid can travel and thus short the nerve/electrode contact. Equally important is the ability of the material to flow into itself and create one continuous mass from underneath the nerve all the way to the top of the nerve/electrode contact to ensure long-term recording stability. Kwik-Cast is color-coded to make the mixing foolproof. The catalyst is yellow and the base is blue. When uniformly mixed, it is green. Kwik-Cast can be applied and cured underneath mineral oil. After recording, electrodes are easily recovered due to the low tear strength. The slight longer curing time (approx. 3 minutes) makes it more suitable for stationary preparations and in vitro tissue studies.
New Tip Design
Kwik-Sil and Kwik-Cast now come with a new tip design. The new tip is engineered to minimize leakage.
The properties of this bio-compatible surgical adhesive make it exceptionally useful for neuroscience applications, peripheral nerve studies and similar biomedical applications. These silicone elastomers also eliminate the mess and time involved in pre-mixing other commonly used formulations (such as Wacker SilGel® and Sylgard®). Each silicone elastomer is packaged in a double barrel syringe and is automatically mixed when pressed out of the mixer tip provided. It can then be applied directly to the tissue without further mixing. The curing time of these silicone adhesives is short, reliable and reproducible. This eliminating potential costly guesswork when using other tissue adhesives. The curing process does not produce any heat, which can cause tissue damage. WPI's silicone elastomers are much less toxic than dental silicone, because they contain no surfactant additives.
The silicone adhesive inside the syringes cannot be sterilized by any means including UV, gamma, H2O2, autoclaving or EtO.
The exterior and the tips can be immersed in or wiped with 70/30 alcohol or you can use a liquid sterilant like Cidex OPA (WPI P/N 7364-4) or Rapicide OPA (WPI P/N 504611).
Q: I want to use KwikSil surgical glue in my "imaging" experiments in rats. How do I get rid of the bubbles completely, or at least reduce them as much as possible?
A: The bubbles are hydrogen gas that is released as a by-product of the curing process. The cure happens in such a short period that the gas can't escape. A slower cure time might help. To slow the cure process, cool the material before mixing.
KWIK-CAST & KWIK-SIL Specifications
KWIK-CAST Silicone Casting Compound (two 5-mL syringes)
KWIK-SIL Silicone Adhesive Compound (two 5-mL syringes)
|MIX RATIO||1 to 1||1 to 1|
|WORKING TIME||< 5 minutes*||4 minutes|
|SETTING TIME (ROOM TEMP., 1:1 RATIO)||5–10 minutes**||<10 minutes|
|CURE TIME||~15 minutes|
|GUARANTEED SHELF LIFE AT 23 °C||6 months||6 months|
|VOLUME||5 mL||5 mL|
|NUMBER OF MIXING TIP||10||10|
|DEAD VOLUME OF THE MIXING TIP||<0.12 mL||<0.12 mL|
|AFTER CURING 24 HOURS:|
|TEAR STRENGTH, PPI||90||44|
|DUROMETER (SHORE A-2)||30||36|
|VOLUME RESISTIVITY, W/CM||1x1015||1x1015|
* 3 minutes average with about 90 seconds of liquidity
** no longer mixable at this point
Fan Y, Jiang E, Hahka T, Chen QH, Yan J, Shan Z. Orexin A increases sympathetic nerve activity through promoting expression of proinflammatory cytokines in Sprague Dawley rats. Acta Physiol (Oxf). 2017 Sep 5. doi: 10.1111/apha.12963. https://www.ncbi.nlm.nih.gov/pubmed/28872777 .
Yang, Z., Xie, W., Ju, F., khan, A., & Zhang, S. (2017). In vivo two-photon imaging reveals a role of progesterone in reducing axonal dieback after spinal cord injury in mice. Neuropharmacology, 116, 30–37. http://doi.org/10.1016/j.neuropharm.2016.12.007
Paoli, M., Andrione, M., & Haase, A. (2017). Imaging Techniques in Insects (pp. 471–519). http://doi.org/10.1007/978-1-4939-6725-4_15
Madden, C. J., Santos da Conceicao, E. P., & Morrison, S. F. (2017). Vagal afferent activation decreases brown adipose tissue (BAT) sympathetic nerve activity and BAT thermogenesis. Temperature, 4(1), 89–96. http://doi.org/10.1080/23328940.2016.1257407
Laszt?czi, B., & Klausberger, T. (2017). Distinct gamma oscillations in the distal dendritic fields of the dentate gyrus and the CA1 area of mouse hippocampus. Brain Structure and Function, 1–11. http://doi.org/10.1007/s00429-017-1421-3
Kawada, T., Turner, M. J., Shimizu, S., Fukumitsu, M., Kamiya, A., & Sugimachi, M. (2017). Aortic depressor nerve stimulation does not impede the dynamic characteristics of the carotid sinus baroreflex in normotensive or spontaneously hypertensive rats. American Journal of Physiology - Regulatory, Integrative and Comparative Physiology, 312(5). Retrieved from http://ajpregu.physiology.org/content/312/5/R787
Bassi, G. S., Dias, D. P. M., Franchin, M., Talbot, J., Reis, D. G., Menezes, G. B., … Kanashiro, A. (2017). Modulation of experimental arthritis by vagal sensory and central brain stimulation. Brain, Behavior, and Immunity, 64, 330–343. http://doi.org/10.1016/j.bbi.2017.04.003
Dai, X., Zhou, W., Gao, T., Liu, J., & Lieber, C. M. (2016). Supplementary Information for: Three-dimensional mapping and regulation of action potential propagation in nanoelectronics innervated tissues Three-dimensional mapping and regulation of action potential propagation in nanoelectronics-innervated tissues. https://doi.org/10.1038/NNANO.2016.96
Balkowiec, A., Kunze, D. L., & Katz, D. M. (n.d.). Brain-Derived Neurotrophic Factor Acutely Inhibits AMPA-Mediated Currents in Developing Sensory Relay Neurons.
Bechtold, A. G., & Scheuer, D. A. (n.d.). Glucocorticoids act in the dorsal hindbrain to modulate baroreflex control of heart rate.
Gao, L., Zhu, Z., Zucker, I. H., & Wang, W. (n.d.). Cardiac sympathetic afferent stimulation impairs baroreflex control of renal sympathetic nerve activity in rats.
Kakegawa, W., Miyazaki, T., Kohda, K., Matsuda, K., Emi, K., Motohashi, J., … Yuzaki, M. (n.d.). The N-Terminal Domain of GluD2 (GluR␦2) Recruits Presynaptic Terminals and Regulates Synaptogenesis in the Cerebellum In Vivo. http://doi.org/10.1523/JNEUROSCI.6013-08.2009
Luckett, B. S., Frielle, J. L., Wolfgang, L., & Stocker, S. D. (n.d.). Arcuate nucleus injection of an anti-insulin affibody prevents the sympathetic response to insulin.
Mizuno, M., Murphy, M. N., Mitchell, J. H., & Smith, S. A. (n.d.). Skeletal muscle reflex-mediated changes in sympathetic nerve activity are abnormal in spontaneously hypertensive rats.
Salgado-Commissariat, D., & Alkadhi, K. A. Serotonin inhibits epileptiform discharge by activation of 5-HT1A receptors in CA1 pyramidal neurons. Neuropharmacology, 36(11–12), 1705–12. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/9517442
Wang, W.-Z., Gao, L., Wang, H.-J., Zucker, I. H., & Wang, W. (n.d.). Interaction between cardiac sympathetic afferent reflex and chemoreflex is mediated by the NTS AT 1 receptors in heart failure.
Xie, C., Sachs, J. R., & Wang, D. H. (n.d.). Interdependent Regulation of Afferent Renal Nerve Activity and Renal Function: Role of Transient Receptor Potential Vanilloid Type 1, Neurokinin 1, and Calcitonin Gene-Related Peptide Receptors. http://doi.org/10.1124/jpet.108.136374
Yamamoto, K., Kawada, T., Kamiya, A., Takaki, H., Miyamoto, T., Sugimachi, M., & Sunagawa, K. (n.d.). Muscle mechanoreflex induces the pressor response by resetting the arterial baroreflex neural arc.
Thompson, Z. S., Rijal, N. P., Jarvis, D., Edwards, A., & Bhattarai, N. (2016). Synthesis of Keratin-based Nanofiber for Biomedical Engineering. Journal of Visualized Experiments, (108), e53381–e53381. http://doi.org/10.3791/53381
Schwarz, M. K., Scherbarth, A., Sprengel, R., Engelhardt, J., Theer, P., Giese, G., … Tomancak, P. (2015). Fluorescent-Protein Stabilization and High-Resolution Imaging of Cleared, Intact Mouse Brains. PLOS ONE, 10(5), e0124650. http://doi.org/10.1371/journal.pone.0124650
Farrar, M. J., & Schaffer, C. B. (2014). A Procedure for Implanting a Spinal Chamber for Longitudinal <em>In Vivo </em>Imaging of the Mouse Spinal Cord. Journal of Visualized Experiments, (94), e52196–e52196. http://doi.org/10.3791/52196
Kawada, T., Li, M., Zheng, C., Shimizu, S., Uemura, K., Turner, M. J., … Sugimachi, M. (2014). Chronic vagal nerve stimulation improves baroreflex neural arc function in heart failure rats Chronic vagal nerve stimulation im- proves baroreflex neural arc function in heart failure rats. J Appl Physiol, 116, 1308–1314. http://doi.org/10.1152/japplphysiol.00140.2014
Kawada, T., Li, M., Zheng, C., Shimizu, S., Uemura, K., Turner, M. J., … Sugimachi, M. (2014). Chronic vagal nerve stimulation improves baroreflex neural arc function in heart failure rats. Journal of Applied Physiology (Bethesda, Md. : 1985), 116(10), 1308–14. http://doi.org/10.1152/japplphysiol.00140.2014
Kislin, M., Mugantseva, E., Molotkov, D., Kulesskaya, N., Khirug, S., Kirilkin, I., … Khiroug, L. (2014). Flat-floored Air-lifted Platform: A New Method for Combining Behavior with Microscopy or Electrophysiology on Awake Freely Moving Rodents. Journal of Visualized Experiments, (88), e51869–e51869. http://doi.org/10.3791/51869
Rodríguez-Contreras, A., Shi, L., & Fu, B. M. (2014). A Method to Make a Craniotomy on the Ventral Skull of Neonate Rodents. Journal of Visualized Experiments, (87), e51350–e51350. http://doi.org/10.3791/51350
Roome, C. J., & Kuhn, B. (2014). Chronic cranial window with access port for repeated cellular manipulations, drug application, and electrophysiology. Frontiers in Cellular Neuroscience, 8, 379. http://doi.org/10.3389/fncel.2014.00379
Zimmermann, J. B., & Jackson, A. (2014). Closed-loop control of spinal cord stimulation to restore hand function after paralysis. Frontiers in Neuroscience, 8, 87. http://doi.org/10.3389/fnins.2014.00087
Fenrich, K. K., Weber, P., Rougon, G., & Debarbieux, F. (2013). Implanting Glass Spinal Cord Windows in Adult Mice with Experimental Autoimmune Encephalomyelitis. Journal of Visualized Experiments, (82), e50826–e50826. http://doi.org/10.3791/50826
Woolley, A. J., Desai, H. A., Gaire, J., Ready, A. L., & Otto, K. J. (2013). Intact Histological Characterization of Brain-implanted Microdevices and Surrounding Tissue. Journal of Visualized Experiments, (72), e50126–e50126. http://doi.org/10.3791/50126
Fenrich, K. K., Weber, P., Hocine, M., Zalc, M., Rougon, G., & Debarbieux, F. (2012). Long-term in vivo imaging of normal and pathological mouse spinal cord with subcellular resolution using implanted glass windows. The Journal of Physiology, 590(Pt 16), 3665–75. http://doi.org/10.1113/jphysiol.2012.230532
Gage, G. J., Stoetzner, C. R., Richner, T., Brodnick, S. K., Williams, J. C., & Kipke, D. R. (2012). Surgical implantation of chronic neural electrodes for recording single unit activity and electrocorticographic signals. Journal of Visualized Experiments : JoVE, (60), e3565. http://doi.org/10.3791/3565
Morgan, S. J., & Paolini, A. G. (2012). Behavioral Determination of Stimulus Pair Discrimination of Auditory Acoustic and Electrical Stimuli Using a Classical Conditioning and Heart-rate Approach. Journal of Visualized Experiments, (64), e3598–e3598. http://doi.org/10.3791/3598
Shi, Z., Chen, W.-W., Xiong, X.-Q., Han, Y., Zhou, Y.-B., Zhang, F., … Zhu, G.-Q. (2012). Sympathetic activation by chemical stimulation of white adipose tissues in rats. J Appl Physiol, 112, 1008–1014. http://doi.org/10.1152/japplphysiol.01164.2011
Silbering, A. F., Bell, R., Galizia, C. G., & Benton, R. (2012). Calcium Imaging of Odor-evoked Responses in the <em>Drosophila</em> Antennal Lobe. Journal of Visualized Experiments, (60), e2976–e2976. http://doi.org/10.3791/2976
Harris, J. P., Capadona, J. R., Miller, R. H., Healy, B. C., Shanmuganathan, K., Rowan, S. J., … Tyler, D. J. (2011). Mechanically adaptive intracortical implants improve the proximity of neuronal cell bodies. Journal of Neural Engineering, 8(6), 66011. http://doi.org/10.1088/1741-2560/8/6/066011
Cullins, M. J., & Chiel, H. J. (2010). Electrode Fabrication and Implantation in <em>Aplysia californica</em> for Multi-channel Neural and Muscular Recordings in Intact, Freely Behaving Animals. Journal of Visualized Experiments, (40), e1791–e1791. http://doi.org/10.3791/1791
Huber, D. A., & Schreihofer, A. M. (2010). Attenuated baroreflex control of sympathetic nerve activity in obese Zucker rats by central mechanisms. The Journal of Physiology, 588(9), 1515–1525. http://doi.org/10.1113/jphysiol.2009.186387
Kamiya, A., Kawada, T., Mizuno, M., Shimizu, S., & Sugimachi, M. (2010). Parallel resetting of arterial baroreflex control of renal and cardiac sympathetic nerve activities during upright tilt in rabbits. Am J Physiol Heart Circ Physiol, 298, 1966–1975. http://doi.org/10.1152/ajpheart.00340.2009
Morgan, D. A., & Rahmouni, K. (2010). Differential effects of insulin on sympathetic nerve activity in agouti obese mice. Journal of Hypertension, 28(9), 1913–9. http://doi.org/10.1097/HJH.0b013e32833c2289
Battaglia, F. P., Kalenscher, T., Cabral, H., Winkel, J., Bos, J., Manuputy, R., … Pennartz, C. (2009). The Lantern: an ultra-light micro-drive for multi-tetrode recordings in mice and other small animals. Journal of Neuroscience Methods, 178(2), 291–300. http://doi.org/10.1016/j.jneumeth.2008.12.024
Bechtold, A. G., Patel, G., Hochhaus, G., & Scheuer, D. A. (2009). Chronic blockade of hindbrain glucocorticoid receptors reduces blood pressure responses to novel stress and attenuates adaptation to repeated stress. Am J Physiol Regul Integr Comp Physiol, 296, 1445–1454. http://doi.org/10.1152/ajpregu.00095.2008
Dombeck, D. A., Graziano, M. S., & Tank, D. W. (2009). Functional clustering of neurons in motor cortex determined by cellular resolution imaging in awake behaving mice. The Journal of Neuroscience : The Official Journal of the Society for Neuroscience, 29(44), 13751–60. http://doi.org/10.1523/JNEUROSCI.2985-09.2009
Haehnel, M., Froese, A., & Menzel, R. (2009). <em>In vivo</em> Ca<sup>2+</sup>- Imaging of Mushroom Body Neurons During Olfactory Learning in the Honey Bee. Journal of Visualized Experiments, (30), e1353–e1353. http://doi.org/10.3791/1353
Jackson, N., & Muthuswamy, J. (2008). Artificial dural sealant that allows multiple penetrations of implantable brain probes. Journal of Neuroscience Methods, 171(1), 147–52. http://doi.org/10.1016/j.jneumeth.2008.02.018
Spitler, K. M., & Gothard, K. M. (2008). A removable silicone elastomer seal reduces granulation tissue growth and maintains the sterility of recording chambers for primate neurophysiology. Journal of Neuroscience Methods, 169(1), 23–6. http://doi.org/10.1016/j.jneumeth.2007.11.026
Pan, Y.-X., Gao, L., Wang, W.-Z., Zheng, H., Liu, D., Patel, K. P., … Wang, W. (2007). Exercise Training Prevents Arterial Baroreflex Dysfunction in Rats Treated With Central Angiotensin II. Hypertension, 49(3).
Kurata, K., Heino, T. J., Higaki, H., & Väänänen, H. K. (2006). Bone Marrow Cell Differentiation Induced by Mechanically Damaged Osteocytes in 3D Gel-Embedded Culture. Journal of Bone and Mineral Research, 21(4), 616–625. http://doi.org/10.1359/jbmr.060106
Sachse, S., Peele, P., Silbering, A. F., Gühmann, M., & Galizia, C. G. (2006). Role of histamine as a putative inhibitory transmitter in the honeybee antennal lobe. Frontiers in Zoology, 3(1), 22. http://doi.org/10.1186/1742-9994-3-22
Barrett, C. J., Guild, S.-J., Ramchandra, R., & Malpas, S. C. (2005). Baroreceptor denervation prevents sympathoinhibition during angiotensin II-induced hypertension. Hypertension, 46(1), 168–72. http://doi.org/10.1161/01.HYP.0000168047.09637.d4
Gao, L., Wang, W., Li, Y.-L., Schultz, H. D., Liu, D., Cornish, K. G., & Zucker, I. H. (2005). Sympathoexcitation by central ANG II: Roles for AT 1 receptor upregulation and NAD(P)H oxidase in RVLM Sympatho- excitation by central ANG II: Roles for AT 1 receptor upregulation and NAD(P)H oxidase in RVLM. Am J Physiol Heart Circ Physiol, 288, 2271–2279. http://doi.org/10.1152/ajpheart.00949.2004
Barann, M., Dilger, J. P., Bönisch, H., Göthert, M., Dybek, A., & Urban, B. W. (2000). Inhibition of 5-HT3 receptors by propofol: equilibrium and kinetic measurements. Neuropharmacology, 39(6), 1064–74. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/10727717
Mire, P., Nasse, J., & Venable-Thibodeaux, S. (2000). Gap junctional communication in the vibration-sensitive response of sea anemones. Hearing Research, 144(1–2), 109–23. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/10831870
Cannon, S. C., & Corey, D. P. (1993). Loss of Na+ channel inactivation by anemone toxin (ATX II) mimics the myotonic state in hyperkalaemic periodic paralysis. The Journal of Physiology, 466, 501–20. Retrieved from http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1175489&tool=pmcentrez&rendertype=abstract