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EVOM™ Electrode for TEER
As low as
$2,250.00
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Prices valid in USA, Canada, and PR only.
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Prices valid in USA, Canada, and PR only.
TEER measurement in individual cups
Using WPI’s EVOM™ Manual resistance meter, Endohm chambers provide reproducible resistance measurements of endothelial and epithelial monolayers in culture cups. Transfer cups from their culture wells to the Endohm chamber for measurement rather than using hand-held electrodes. The chamber and the cap each contain a pair of concentric electrodes: a voltage-sensing silver/silver chloride pellet in the center plus an annular current electrode. The height of the top electrode can be adjusted to fit cell culture cups of different manufacture.
To learn more about our warranty options, click here.
Prices valid in USA, Canada, and PR only.
For TEER measurement of epithelial and endothelial cell cultures
- The new EndOhm chamber upper mount is made of polycarbonate and unaffected by alcohol
- The glass chamber is easier to clean and more scratch resistant than the prior versions. The EndOhm is not recommended for incubator use due to risk of the glass cracking.
- Adjustable apical electrode height
- Crystal clear glass chamber allows visualization of apical electrode positioning
- New insert holder with 120º tri-supports for three leg inserts
- Three sizes cover a range of well cup sizes from a variety of manufacturers
- Compatible with EVOM3 and EVOM™ Manual
To find the equivalent EndOhm chambers compatible with EVOM, EVOM2™, and Millicell® ERS and ERS2, visit the product page here.
Options
| Order Code | Description |
| EVM-EL-03-01-01 | ENDOHM-6 EVOM™ Electrode for TEER in 6.5 mm Insert |
| EVM-EL-03-01-02 | ENDOHM-12 EVOM™ Electrode for TEER in 12 mm Insert |
| EVM-EL-03-01-03 | ENDOHM-24 EVOM™ Electrode for TEER in 24 mm Insert |
Benefits
- Stability and reproducibility superior to the STX4 electrodes to 1% tolerance
- Can be used with 6, 12 or 24 well plates with removable inserts
- Symmetrical electrode pattern disperses test current uniformly
- Tri-leg supports offer mechanical stability and the membrane is held parallel to the electrodes
- Simple test procedure to verify electrode performance
- Premium Warranty Available
Applications
- TEER measurement for removable culture cup systems using EVOM™ Manual meters for endothelial and epithelial cell cultures
Make more precise measurements with EndOhms
EndOhm’s symmetrically opposing circular disc electrodes, situated above and beneath the membrane, allow a more uniform current density to flow across the membrane than with the basic STX4 electrodes. The background resistance of a blank insert is reduced from 150 Ω (when using WPI’s hand-held STX4 electrodes) to less than 5 Ω. With EndOhm’s fixed electrode geometry, variation of readings on a given sample is reduced from 10-30 Ω with STX4 electrodes (depending on the experience of the user) to 1-2 Ω. Compared with other resistance measurement methods, EndOhm with an EVOM meter offers a much more convenient and economic solution to “leaky tissue” measurement. Because of the uniform density of the AC square wave current from EVOM™ Manual, errors owing to electrode polarization or membrane capacitance are largely eliminated. EndOhm together with EVOM™ Manual offers the most accurate and economical endothelial ohmmeter now available. To date, cups from Corning, Millipore, Nunc, Greiner and BD Falcon have been tested. EndOhm chambers may be sterilized with EtO, alcohol or a bactericide; not autoclavable.
NOTE: EndOhm chambers have Ag/AgCl electrodes. If you are planning to do continuous data recording for extended period, you may need to take into consideration any potential cytotoxic effects of long-term exposure of silver on your specific cell type.
Compatibility Charts
The ENDOHM-6 (#EVM-EL-03-01-01) is compatible with the following chambers:
| Corning | Millipore | Material | Membrane Diameter (mm) | Growth Surface Area (cm²) | Membrane Pore Size (μm) |
| 3470 | 6.5 | 0.33 | 0.4 | ||
| 3472 | PITP01250 | 6.5 | 0.33 | 3.0 | |
| 3413 | PCF Insert | 6.5 | 0.33 | 0.4 | |
| 3415 | PITP 01250 PCF Insert |
6.5 | 0.33 | 3.0 | |
| 3421 | 6.5 | 0.33 | 5.0 | ||
| 3422 | PIEP 01250 PCF Insert |
6.5 | 0.33 | 8.0 | |
| 3495 | PIHT12R48* PET Insert |
6.5 | 0.33 | 0.4 | |
| PIHA012 50 | HA Insert | 6.5 | 0.33 | 0.45 | |
| PICM012 50 | CM Insert | 6.5 | 0.33 | 0.4 | |
| 3496 | PISP12R48* PET Insert |
6.5 | 0.33 | 3.0 | |
| PIRP12R48* PET Insert |
6.5 | 0.33 | 1.0 | ||
| PIMP12R48* PET Insert |
6.5 | 0.33 | 5.0 | ||
| PIEP12R48* PET Insert |
6.5 | 0.33 | 8.0 | ||
| PIXP01250 PCF Insert |
6.5 | 0.33 | 12 | ||
| PIHP01250 | 1.0 | ||||
| PITT01250 | 3.0 |
* The tri-supports overhang the chamber edge and the well cannot be held parallel to the electrodes.
| Nunc | Pore size (μm) | Culture area (cm²) |
| 140620 | 0.4 | 0.47 |
| 140627 | 3.0 | 0.47 |
| 140629 | 8.0 | 0.47 |
| ThinCertTM | Membrane material | Pore size [µm] | Pore density [cm-2] | Optical membrane properties | TC surface treatment/Sterile | Multiwell plates/ThinCertTM per box |
| 662640 | PET | 0.4 | 1 x 108 | translucent | +/+ | 2/48 |
| 662641 | PET | 0.4 | 2 x 106 | transparent | +/+ | 2/48 |
| 662610 | PET | 1.0 | 2 x 106 | transparent | +/+ | 2/48 |
| 662630 | PET | 3.0 | 0.6 x 106 | transparent | +/+ | 2/48 |
| 662631 | PET | 3.0 | 2 x 106 | translucent | +/+ | 2/48 |
| 662638 | PET | 8.0 | 0.15 x 106 | translucent | +/+ | 2/48 |
| Millicell | Pore size (μm) | Qty/pk |
| MCHT24H48 | 0.4 | 48 |
| MCRP24H48 | 1.0 | 48 |
| MCSP24H48 | 3.0 | 48 |
| MCMP24H48 | 5.0 | 48 |
| MCEP24H48 | 8.0 | 48 |
| BD Falcon | Membrane material | Pore size [µm] | Pore density [cm-2] | Optical membrane properties | TC plate (#wells) |
| 353095 | PET | 0.4 | 2.0 ± 0.2 x 106 | transparent | 24 |
| 353104 | PET | 1.0 | 1.6 ± 0.6 x 106 | transparent | 24 |
| 353096 | PET | 3.0 | 8 ± 2 x 105 | transparent | 24 |
| 353097 | PET | 8.0 | 6 ± 2 x 104 | translucent | 24 |
| 353495 | PET | 0.4HD | 100 ± 10 x 106 | translucent | 24 |
| 353492 | PET | 3.0HD | 2.0 ± 0.2 x 105 | translucent | 24 |
The ENDOHM-12 (#EVM-EL-03-01-02) is compatible with the following chambers:
| Corning | Millipore | Membrane Diameter (mm) | Growth Surface Area (cm²) | Membrane Pore Size (μm) |
| 3401 | 12 | 1.12 | 0.4 | |
| 3402 | PITP01250 | 12 | 1.12 | 3.0 |
| PITT01250 | 12 | 1.12 | 3.0 | |
| 3493 | 12 | 1.12 | 0.4 | |
| 3494 | 12 | 1.12 | 3.0 | |
| 3460 | PIHT15R48* PET Insert |
12 | 1.12 | 0.4 |
| PIRP15R48* PET Insert |
12 | 1.12 | 1.0 | |
| 3462 | PISP15R48* PET Insert |
12 | 1.12 | 3.0 |
| PIMP15R48* PET Insert |
12 | 1.12 | 5.0 | |
| PIEP30R48* PIEP15R48* PET Insert |
12 | 1.12 | 8.0 |
* The tri-supports legs must be balance correctly so that the filter is parallel to the electrodes.
| Nunc | Pore size (μm) | Culture area (cm²) |
| 140652 | 0.4 | 1.13 |
| 140654 | 3.0 | 1.13 |
| 140656 | 8.0 | 1.13 |
| ThinCertTM | Membrane material | Pore size [µm] | Pore density [cm-2] | Optical membrane properties | TC surface treatment/Sterile | Multiwell plates/ThinCertTM per box |
| 665640 | PET | 0.4 | 1 x 108 | translucent | +/+ | 4/48 |
| 665641 | PET | 0.4 | 2 x 106 | transparent | +/+ | 4/48 |
| 665610 | PET | 1.0 | 2 x 106 | transparent | +/+ | 4/48 |
| 665630 | PET | 3.0 | 0.6 x 106 | transparent | +/+ | 4/48 |
| 665631 | PET | 3.0 | 2 x 106 | translucent | +/+ | 4/48 |
| 665638 | PET | 8.0 | 0.15 x 106 | translucent | +/+ | 4/48 |
| Millicell | Pore size (μm) | Qty/pk |
| MCHT12H48 | 0.4 | 48 |
| MCRP12H48 | 1.0 | 48 |
| MCSP12H48 | 3.0 | 48 |
| MCMP12H48 | 5.0 | 48 |
| MCEP12H48 | 8.0 | 48 |
| BD Falcon | Membrane material | Pore size [µm] | Pore density [cm-2] | Optical membrane properties | TC plate (#wells) |
| 353180 | PET | 0.4 | 2.0 ± 0.2 x 106 | transparent | 12 |
| 353103 | PET | 1.0 | 1.6 ± 0.6 x 106 | transparent | 12 |
| 353181 | PET | 3.0 | 8 ± 2 x 105 | transparent | 12 |
| 353182 | PET | 8.0 | 6 ± 2 x 104 | translucent | 12 |
| 353494 | PET | 0.4HD | 100 ± 10 x 106 | translucent | 12 |
| 353292 | PET | 3.0HD | 2.0 ± 0.2 x 105 | translucent | 12 |
The ENDOHM-24 (#EVM-EL-03-01-03) is compatible with the following chambers:
| Corning | Millipore | Membrane Material | Pore Size (µm) |
| 3407 | Polycarbonate | 0.4 | |
| 3801 | Polycarbonate | 0.4 | |
| Polycarbonate | 3.0 | ||
| 3412 | PIHT30R48* | Polycarbonate | 0.4 |
| 3414 | Polycarbonate | 3.0 | |
| PITT03050 | Polycarbonate | 3.0 | |
| 3428 | Polycarbonate | 8.0 | |
| 3450 | Polyester | 0.4 | |
| 3452 | Polyester | 3.0 | |
| 3491 | Collagen | 0.4 | |
| 3492 | Collagen | 3.0 | |
| PICMORG50 | Organotypic Insert | 0.4 | |
| PIHA03050 | HA Insert | 0.45 | |
| PIHP03050 | PCF Insert | 0.4 | |
| PICM03050 | HA mixed cellulose esters | 0.4 | |
| PIHT30R48* | PET Insert | 0.4 | |
| PIRP30R48* | PET Insert | 1.0 | |
| PISP30R48* | PET Insert | 3.0 | |
| PIMP30R48* | PET Insert | 5.0 | |
| PIEP30R48* | PET Insert | 8.0 |
* The tri-support legs must be balanced correctly so that the filter is parallel to the electrodes.
| Nunc | Pore size (μm) | Culture area (cm²) |
| 140640 | 0.4 | 3.14 |
| 140642 | 3.0 | 3.14 |
| 140644 | 8.0 | 3.14 |
| 140660 | 0.4 | 4.1 |
| 140663 | 3 | 4.1 |
| 140668 | 8 | 4.1 |
| ThinCertTM | Membrane material | Pore size [µm] | Pore density [cm-2] | Optical membrane properties | TC surface treatment/Sterile | Multiwell plates/ThinCertTM per box |
| 657640 | PET | 0.4 | 1 x 108 | translucent | +/+ | 4/24 |
| 657641 | PET | 0.4 | 2 x 106 | transparent | +/+ | 4/24 |
| 657610 | PET | 1.0 | 2 x 106 | transparent | +/+ | 4/24 |
| 657630 | PET | 3.0 | 0.6 x 106 | transparent | +/+ | 4/24 |
| 657631 | PET | 3.0 | 2 x 106 | translucent | +/+ | 4/24 |
| 657638 | PET | 8.0 | 0.15 x 106 | translucent | +/+ | 4/24 |
| Millicell | Pore size (μm) | Qty/pk |
| MCHT06H48 | 0.4 | 48 |
| MCRP06H48 | 1.0 | 48 |
| MCSP06H48 | 3.0 | 48 |
| MCMP06H48 | 5.0 | 48 |
| MCEP06H48 | 8.0 | 48 |
| BD Falcon | Membrane material | Pore size [µm] | Pore density [cm-2] | Optical membrane properties | TC plate (#wells) |
| 353090 | PET | 0.4 | 2.0 ± 0.2 x 106 | transparent | 6 |
| 353102 | PET | 1.0 | 1.6 ± 0.6 x 106 | transparent | 6 |
| 353091 | PET | 3.0 | 8 ± 2 x 105 | transparent | 6 |
| 353093 | PET | 8.0 | 6 ± 2 x 104 | translucent | 6 |
| 353493 | PET | 0.4HD | 100 ± 10 x 106 | translucent | 6 |
| 353092 | PET | 3.0HD | 2.0 ± 0.2 x 105 | translucent | 6 |
| SKU | VAR-evm-el-03-01-01 |
|---|
Related Articles
Sheller, R. A., Cuevas, M. E., & Todd, M. C. (2017). Comparison of transepithelial resistance measurement techniques: Chopsticks vs. EndOhm. Biological Procedures Online, 19, 4. http://doi.org/10.1186/s12575-017-0053-6
Srinivasan, B., Kolli, A. R., Esch, M. B., Abaci, H. E., Shuler, M. L., & Hickman, J. J. (2015). TEER measurement techniques for in vitro barrier model systems. Journal of Laboratory Automation, 20(2), 107–26. http://doi.org/10.1177/2211068214561025
TORRES, R., PIZARRO, L., CSENDES, A., GARCÍA, C., LAGOS, N., Pasdar, M., … Roskelley, C. (2007). GTX 2/3 EPIMERS PERMEATE THE INTESTINE THROUGH A PARACELLULAR PATHWAY. The Journal of Toxicological Sciences, 32(3), 241–248. http://doi.org/10.2131/jts.32.241
Patil, R. V., Han, Z., Yiming, M., Yang, J., Iserovich, P., Wax, M. B., & Fischbarg, J. (2001). Fluid transport by human nonpigmented ciliary epithelial layers in culture: a homeostatic role for aquaporin-1. American Journal of Physiology - Cell Physiology, 281(4).
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