Low Volume Flow Cell
For FIA, HPLC and process analysis
- UV/VIS flow cell for absorbance
- Low internal volume
- Fits 500 and 600 µm fibers
- High UV transmission
- 0–10 mL/min flow rate
|Optical Pathlength||10 mm||50 mm||100 mm|
|Internal Volume||2.4 μL||12 µL||24µL|
|Refractive Index@ 280nm (measured using ASTM E 685-93)||< 7 mAU||<15 mAU||<30 mAU|
|Transmission @ 254nm (Reference:2 600μm fiber, butt-connected)||25%||20%||15%|
|Transmission @ 500nm (Reference:2 600μm fiber, butt-connected)||40%||35%||30%|
|Wavelength Range||200-1000 nm|
|Fiber Connection [μm]||500 (SMA)|
|Maximum Pressure||50 bar|
|Wetted Materials||PEEK, Fused Silica, PTFE|
- High efficiency coupling
- Low refractive index offset
- Fits WPI LEDSpec and TIDAS systems
- FIA, GFIA, HPLC, Optofluidics
- Process control
- DNA, RNA and protein quantification
- Colorimetric nutrient and trace metal analysis
- Drug discovery and dissolution testing
MicroLWCC is a new fiber optic low volume flow cell for UV/VIS/NIR absorbance analysis. Based on WPI’s established liquid core waveguide technology, the analyte solution functions as the core of a fluid filled light waveguide. Wetted parts in the sample cell light path are PEEK, fused silica and PTFE. Optical fibers are used to transport light to and from the sample cell. The cell can be used in biochemistry for DNA, RNA & protein quantification, colorimetric nutrient and trace metal analysis, drug discovery and dissolution testing, process control, and HPLC analysis.
M. Belz, "Simple and sensitive protein detection system using UV LEDs and liquid core waveguides", Advanced Environmental, Chemical, and Biological Sensing Technologies V, Optics East, Oct 2007, Proc. SPIE, Vol. 6755, 675505.
M. Belz, F.A. Klein, H.S. Eckhardt, K. Klein, D. Dinges, K.T.V. Grattan, "Optical Detection Techniques and Light Delivery with UV LEDs and Optical Fibres", Third International Conference on Optical and Laser Diagnostics, Proc. IOP, City University, London, UK, May 2007.
M. Belz, P. Dress, A. Sukhitskiy, S. Liu, "Linearity and effective optical pathlength of liquid waveguide capillary cells", Part of the SPIE Conference on Internal Standardization and Calibration; Architectures for Chemical Sensors, Boston, Massachusetts, September 1999, SPIE Vol. 3856, 271-281.
Fischer, Lee Mackenzie et al. 2013. “Exploration of Two Methods for Quantitative Mitomycin C Measurement in Tumor Tissue in Vitro and in Vivo.” Biological procedures online 15(1): 12. http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=3831870&tool=pmcentrez&rendertype=abstract (October 9, 2015).