Constant Current Stimulus Isolator

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Automated bipolar pulsing for zero net charge on biological preparations

  • Constant current
  • Unipolar and bipolar stimulation modes
  • Built-in non-compliance alarm
  • Input is optically isolated
  • Standard TTL triggering
  • DC test mode
  • Powered by 9 V alkaline or rechargeable batteries



Part # Description Battery Type Includes Charger
A365RC A365R with an A362 Battery Charger Rechargeable Battery Yes
SYS-A365R High Voltage Isolator, Bipolar Rechargeable Battery No
SYS-A365D High Voltage Isolator, Bipolar Alkaline Batteries _



  • Compliance voltage is 100V or better
  • Bipolar mode auto generates alternating positive and negative pulses from TTL input
  • Test mode simplifies performance verification
  • Optical isolation enhances safety of the preparation and reduces noise susceptibility


  • Electrophysiology
  • Brain slice stimulation
  • In vivo brain and CNS stimulation

Activated by conventional logic-level commands, Model A365 can be gated by any pulse generator, stimulator or computer output with automated bipolar pulsing for zero net charge on biological preparations.

Dual tone audible alarms

A tone sounds when an open electrode circuit is detected or when system compliance is reached. A second optional tone sounds when a signal is applied to the input. A test switch is also provided to check battery charge.

Current delivery up to 10 mA at more than 100V

Stimulus currents are set using a three-digit control knob and a three-position range switch. Output current tracks control set­tings to better than 1%. Output current is load independent, and voltage suf­fi­cient to push the desired current through the load is automatically de­vel­oped, sub­ject only to compliance limits. Model A360LA produces up to 10 mA current, in three ranges, at more than 100 V compliance.

Bipolar Output Polarity

Output polarity is determined by a push switch on the front panel. Bipolar current is toggled by the command waveform, setting al­ter­nat­ing pulses as positive or negative.


This A365RC Stimulus Isolator includes both the A365R Stimulus Isolator and the A362 battery charger.The rechargeable A365R is supplied with a nickel metal hydride battery stack. The A362 Battery Charger is required with the A365R

 NOTE: Not intended for human use.

OUTPUT WAVEFORM DC or current pulse
OUTPUT CURRENT RANGES 0.1, 1.0, and 10 mA
CURRENT AMPLITUDE ERROR 0.5% of full scale, max.
CURRENT RESOLUTION 0.1% of full scale, typical
EXTERNAL COMMAND THRESHOLD 5 V at 3 mA, min. 10 V, max.
OUTPUT POLARITY Reversible, manual switch or automatic
CURRENT RISE TIME & DELAY 6 μs, typical (1 KΩ load)
CURRENT FALL TIME & DELAY 10 μs, typical (1 KΩ load)
OPTOCOUPLER 2500 V, rated min. breakdown voltage
POWER: Model A365D (dry cell) 16 alkaline 9 V batteries, included
POWER: Model A365R (rechargeable) 16 rechargeable NiMH 9 V batteries included
DIMENSIONS 8.5 x 3.5 x 5 in. (22 x 9 x 12 cm)
SHIPPING WEIGHT 4 lb. (1.8 kg)

Yavich, L., Tanila, H., Vepsäläinen, S., & Jäkälä, P. (n.d.). Neurobiology of Disease Role of ␣-Synuclein in Presynaptic Dopamine Recruitment.

Iremonger, K. J., Anderson, T. R., Hu, B., & Kiss, Z. H. T. (n.d.). Cellular Mechanisms Preventing Sustained Activation of Cortex During Subcortical High-Frequency Stimulation.

Rowland, N. C., & Jaeger, D. (n.d.). Responses to Tactile Stimulation in Deep Cerebellar Nucleus Neurons Result From Recurrent Activation in Multiple Pathways.

D ’ambrosio, R., Gordon, D. S., Winn, H. R., ’ambrosio, D., Raimondo, D. S., Gordon, H., & Richard, W. (n.d.). Differential Role of KIR Channel and Na ϩ /K ϩ -Pump in the Regulation of Extracellular K ϩ in Rat Hippocampus.

Huda, R., Mccrimmon, D. R., & Martina, M. (n.d.). pH modulation of glial glutamate transporters regulates synaptic transmission in the nucleus of the solitary tract.

Chen, Y., Beffert, U., Ertunc, M., Tang, T.-S., Kavalali, E. T., Bezprozvanny, I., & Herz, J. (n.d.). Development/Plasticity/Repair Reelin Modulates NMDA Receptor Activity in Cortical Neurons.

D ’ambrosio, R., Wenzel, J., Schwartzkroin, P. A., Mckhann Ii, G. M., & Janigro, D. (n.d.). Functional Specialization and Topographic Segregation of Hippocampal Astrocytes.

D ’ambrosio, R., Maris, D. O., Grady, M. S., Winn, H. R., & Janigro, D. (n.d.). Impaired K ؉ Homeostasis and Altered Electrophysiological Properties of Post-Traumatic Hippocampal Glia.

Ji, H., & Shepard, P. D. (n.d.). Behavioral/Systems/Cognitive Lateral Habenula Stimulation Inhibits Rat Midbrain Dopamine Neurons through a GABA A Receptor-Mediated Mechanism.

Lee, E., Hong, J., Park, Y.-G., Chae, S., Kim, Y., & Kim, D. (2015). Left brain cortical activity modulates stress effects on social behavior. Scientific Reports, 5, 13342.

Gindrat, A.-D., Quairiaux, C., Britz, J., Brunet, D., Lanz, F., Michel, C. M., & Rouiller, E. M. (2015). Whole-scalp EEG mapping of somatosensory evoked potentials in macaque monkeys. Brain Structure & Function, 220(4), 2121–2142.

Avila, I., & Lin, S.-C. (2014). Motivational Salience Signal in the Basal Forebrain Is Coupled with Faster and More Precise Decision Speed. PLoS Biology, 12(3), e1001811.

Nguyen, D. P., & Lin, S.-C. (2014). A frontal cortex event-related potential driven by the basal forebrain. ELife, 3, e02148. Retrieved from

Herrera, C., Directores, R., Panetsos, F., Carlos, P., & Trueba, A. (2014). TESIS DOCTORAL Efectos de la estimulación artificial de un nervio periférico seccionado sobre la vía somatosensorial desaferentizada de la rata.

Younce, J. R., Albaugh, D. L., & Shih, Y.-Y. I. (2014). Deep Brain Stimulation with Simultaneous fMRI in Rodents. Journal of Visualized Experiments, (84), e51271–e51271.

Oulad Ben Taib, N., & Manto, M. (2013). Trains of Epidural DC Stimulation of the Cerebellum Tune Corticomotor Excitability. Neural Plasticity, 2013(10), 1–12.

Syvänen, S., Russmann, V., Verbeek, J., Eriksson, J., Labots, M., Zellinger, C., … Potschka, H. (2013). [11C]quinidine and [11C]laniquidar PET imaging in a chronic rodent epilepsy model: Impact of epilepsy and drug-responsiveness. Nuclear Medicine and Biology, 40(6), 764–775.

Schroder, E. A., Lefta, M., Zhang, X., Bartos, D. C., Feng, H.-Z., Zhao, Y., … Delisle, B. P. (2013). The cardiomyocyte molecular clock, regulation of Scn5a, and arrhythmia susceptibility. American Journal of Physiology. Cell Physiology, 304(10), C954-65.

Schmuckermair, C., Gaburro, S., Sah, A., Landgraf, R., Sartori, S. B., & Singewald, N. (2013). Behavioral and neurobiological effects of deep brain stimulation in a mouse model of high anxiety- and depression-like behavior. Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology, 38(7), 1234–1244.

Dalby-Brown, W., Jessen, C., Hougaard, C., Jensen, M. L., Jacobsen, T. A., Nielsen, K. S., … Jørgensen, S. (2013). Characterization of a novel high-potency positive modulator of Kv7 channels. European Journal of Pharmacology, 709(1–3), 52–63.

Licko, T., Seeger, N., Zellinger, C., Russmann, V., Matagne, A., & Potschka, H. (2013). Lacosamide treatment following status epilepticus attenuates neuronal cell loss and alterations in hippocampal neurogenesis in a rat electrical status epilepticus model. Epilepsia, 54(7), 1176–1185.

Atherton, J. F., Menard, A., Urbain, N., & Bevan, M. D. (2013). Short-term depression of external globus pallidus-subthalamic nucleus synaptic transmission and implications for patterning subthalamic activity. The Journal of Neuroscience : The Official Journal of the Society for Neuroscience, 33(17), 7130–7144.

Saha, D., Leong, K., Katta, N., & Raman, B. (2013). Multi-unit Recording Methods to Characterize Neural Activity in the Locust (<em>Schistocerca Americana</em>) Olfactory Circuits. Journal of Visualized Experiments, (71), e50139–e50139.

Huda, R., McCrimmon, D. R., & Martina, M. (2013). pH modulation of glial glutamate transporters regulates synaptic transmission in the nucleus of the solitary tract. Journal of Neurophysiology, 110(2), 368–377.

Sonner, P. M., & Ladle, D. R. (2013). Early postnatal development of GABAergic presynaptic inhibition of Ia proprioceptive afferent connections in mouse spinal cord. Journal of Neurophysiology, 109(8), 2118–2128.

Zhu, Z., Sierra, A., Burnett, C. M.-L. L., Chen, B., Subbotina, E., Koganti, S. R. K., … Zingman, L. V. (2013). Sarcolemmal ATP-sensitive potassium channels modulate skeletal muscle function under low-intensity workloads. The Journal of General Physiology, 143(1), 119–134.

Brenowitz, S. D., & Regehr, W. G. (2012). Presynaptic imaging of projection fibers by in vivo injection of dextran-conjugated calcium indicators. Cold Spring Harbor Protocols, 2012(4), 465–471.

Li, W., Janardhan, A. H., Fedorov, V. V, Sha, Q., Schuessler, R. B., & Efimov, I. R. (2011). Low-energy multistage atrial defibrillation therapy terminates atrial fibrillation with less energy than a single shock. Circulation. Arrhythmia and Electrophysiology, 4(6), 917–925.

Mathis, D. M., Furman, J. L., & Norris, C. M. (2011). Preparation of Acute Hippocampal Slices from Rats and Transgenic Mice for the Study of Synaptic Alterations during Aging and Amyloid Pathology. Journal of Visualized Experiments, (49), e2330–e2330.

Manto, M. U., Hampe, C. S., Rogemond, V., & Honnorat, J. (2011). Respective implications of glutamate decarboxylase antibodies in stiff person syndrome and cerebellar ataxia. Orphanet Journal of Rare Diseases, 6(1), 3.

Kim, J., Woo, J., Park, Y.-G., Chae, S., Jo, S., Choi, J. W., … Kim, D. (2011). Thalamic T-type Ca2+ channels mediate frontal lobe dysfunctions caused by a hypoxia-like damage in the prefrontal cortex. The Journal of Neuroscience : The Official Journal of the Society for Neuroscience, 31(11), 4063–4073.

Seo, J. H., Jang, I. K., Kim, H., Yang, M. S., Lee, J. E., Kim, H. E., … Cho, S.-R. (2011). Early Immunomodulation by Intravenously Transplanted Mesenchymal Stem Cells Promotes Functional Recovery in Spinal Cord Injured Rats. Cell Medicine, 2(2), 55–67.

Pelkonen, A., Hiltunen, M., Kiianmaa, K., & Yavich, L. (2010). Stimulated dopamine overflow and alpha-synuclein expression in the nucleus accumbens core distinguish rats bred for differential ethanol preference. Journal of Neurochemistry, 114(4), 1168–1176.

Foust, A. J., Schei, J. L., Rojas, M. J., & Rector, D. M. (2008). In vitro and in vivo noise analysis for optical neural recording. Journal of Biomedical Optics, 13(4), 044038.

Schei, J. L., McCluskey, M. D., Foust, A. J., Yao, X.-C., & Rector, D. M. (2008). Action potential propagation imaged with high temporal resolution near-infrared video microscopy and polarized light. NeuroImage, 40(3), 1034–1043.

Foust, A. J., & Rector, D. M. (2007). Optically teasing apart neural swelling and depolarization. Neuroscience, 145(3), 887–899.

Ji, H., & Shepard, P. D. (2007). Lateral habenula stimulation inhibits rat midbrain dopamine neurons through a GABA(A) receptor-mediated mechanism. The Journal of Neuroscience : The Official Journal of the Society for Neuroscience, 27(26), 6923–6930.

Lee, B. H., Lee, K. H., Yoon, D. H., Kim, U. J., Hwang, Y. S., Park, S. K., … Jahng, T. (2005). Effects of methylprednisolone on the neural conduction of the motor evoked potentials in spinal cord injured rats. Journal of Korean Medical Science, 20(1), 132–138.

Anderson, T., Hu, B., Pittman, Q., & Kiss, Z. H. T. (2004). Mechanisms of deep brain stimulation: an intracellular study in rat thalamus. The Journal of Physiology, 559(1), 301–313.

Sokolow, S., Manto, M., Gailly, P., Molgó, J., Vandebrouck, C., Vanderwinden, J.-M., … Schurmans, S. (2004). Impaired neuromuscular transmission and skeletal muscle fiber necrosis in mice lacking Na/Ca exchanger 3. Journal of Clinical Investigation, 113(2), 265–273.

D’Ambrosio, R., Fairbanks, J. P., Fender, J. S., Born, D. E., Doyle, D. L., & Miller, J. W. (2004). Post-traumatic epilepsy following fluid percussion injury in the rat. Brain : A Journal of Neurology, 127(Pt 2), 304–314.

Shaw, B. K., & Kennedy, G. G. (2002). Evidence for species differences in the pattern of androgen receptor distribution in relation to species differences in an androgen-dependent behavior. Journal of Neurobiology, 52(3), 203–220.

Yavich, L., & Tiihonen, J. (2000). Ethanol modulates evoked dopamine release in mouse nucleus accumbens: dependence on social stress and dose. European Journal of Pharmacology, 401(3), 365–373. Retrieved from

Knisley, S. B., Trayanova, N., & Aguel, F. (1999). Roles of Electric Field and Fiber Structure in Cardiac Electric Stimulation. Biophysical Journal, 77(3), 1404–1417.


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