PC-controlled high throughput TEER measurement for epithelial monolayer

The REMS AutoSampler automates measurements of TEER epithelial or endothelial monolayers cultured on HTS well plates. It is a PC-controlled tissue resistance measurement system that offers reproducibility, accuracy, flexibility and ease-of-operation. Automated measurement of tissue resistance in cell culture microplates provides the advantages of speed, precision, decreased opportunity for contamination and the rapid availability of measured resistance data.

Features

• PC controlled positioning
• Data acquisition in LabView
• Manufacturer specific electrodes available for 24 and 96-well HTS plates
• Plate configuration files and sample sequences are user definable
• Two user-defined rinse locations
• Manual mode 

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Benefits

• Speed—capable of acquiring TEER data on a 96 well plate in less than five minutes
• Automation reduces the possibility for human error

Applications

• Automated measurement and data logging of TEER for 24 and 96 well HTS culture plates

The REMS AutoSampler automates measurements of TEER epithelial or endothelial monolayers cultured on HTS well plates. It is a PC-controlled tissue resistance measurement system that offers reproducibility, accuracy, flexibility and ease-of-operation. Automated measurement of tissue resistance in cell culture microplates provides the advantages of speed, precision, decreased opportunity for contamination and the rapid availability of measured resistance data.

The main components of the REMS AutoSampler include: the robotic sampler that moves the electrode over each well of the microplate, the electrode which is located on the robotic arm, a base plate for the 24 and 96 well tray, a Windows-based data acquisition card, the REMS electrode interface unit and the REMS software to operate the system on a Windows-based computer.

Automate TEER measurements

The REMS AutoSampler automates TEER measurements that would otherwise be performed manually with WPI’s EVOM2™ Epithelial Voltohmmeter. Automated tissue resistance measurements up to 20 kΩ can be performed on 24 or 96 well HTS microplates. See WPI’s website for manufacturer plate compatibility.

Precisely and reproducibly positions electrode

The REMS AutoSampler will automatically measure and record tissue resistance from a user-specified matrix of culture wells on the microplate. According to the specified sequence, the robotic arm moves over the identified wells taking TEER measurements. By means of an x-y-z locating system, the electrode is positioned precisely into the well. The ability of the REMS AutoSampler to reproducibly position the electrode contributes to consistent TEER measurements. TEER data is incrementally stored as the electrode moves from one well to the next.

Compact electrode pair

The use of AC current to measure resistance provides several advantages over DC current, including:

• Absence of offset voltages on measurements
• There is a zero net current being passed through the membrane and, therefore, it is not adversely affected by a current charge
• No electrochemical deposition of electrode metal.

Rinse and calibration check stations

The REMS AutoSampler also features two rinse stations. If occasional rinsing of the REMS electrode is required it may be sent to a rinse station by pressing the rinse station button on the menu bar.

Compatible Plates

The following plates can be used with the REMS system:

Sample Plates

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This is a Corning #3378 24-well plate that may be used with the REMS-24 or the STX100C. The Corning Falcon 24-well plate is virtually identical to this one. For use with REMS-24 or STX100F.	This is a Corning Falcon 96-well plate that may be used with the REMS-96.	This is a Millipore Multiscreen CaCo2 that may be used with the REMS-96 or the STX100M.	This is a Corning 3391 HTS Traswell 96 plate that may be used with the REMS-96C or the STX100C96.	This is a Millicell-24 Cell Culture Insert Plate that may be used with the REMS-24M