- Microprocessor controlled
- Program sequences up to four steps
- Produce micropipettes with a tip diameter less than 0.1µm or greater than 10µm
- Store up to 95 programs in memory
- Multiple factory programs installed
- Tempered glass cover to reduce the effects of humidity on puller reproducibility
- Switchable power supply for any line voltage 90–240VAC ensures that line voltage fluctuations don’t affect reproducibility
- CE approved
For pricing, Customers outside of the US and Canada, please
See the current Data Sheet.
PUL-1000 is a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes used in intracellular recording, patch clamp studies, microperfusion or microinjection. The puller was designed with tight mechanical specifications and precision electronics for complete control of the pulling process and accurate reproducibility. It offers programmable sequences of up to four steps with heating, force, movement and cooling time. This allows graduated cycles for applications like patch clamp recording.
The video below gives a brief overview of this microelectrode/micropipette puller and its features.
A glass capillary is heated by a platinum/iridium filament and pulled by a controlled force. PUL-1000 features permanent memory storage for up to 95 heat programs. It is remarkable in the flexibility and capability of producing a vast array of pipette shapes.
Pulling pipettes is an art, and reliable results depend on factors like the operating environment, the type of glass used and your technique. Understanding how the puller works is critical to manufacturing the pipettes you want.
PUL-1000 can produce pipettes with tip diameters from less than 0.1µm to 10+ µm. Microprocessor settings control the pulling automatically.
The settings for both stages can be stored in memory. Up to 95 user-selectable programs can be stored for later recall. The instrument contains two factory installed and tested programs. Choose from the factory installed programs or create your own.
The cover of pulling chamber is made with tempered glass to minimize the humidity effect on the reproducibility of pulled pipettes.
PUL-1000 has a high quality switching power supply for use anywhere in the world without worry about the line voltage differences. Pulling reproducibility is unaffected by line voltage fluctuation. Heating voltage can be controlled to within 0.1% accuracy even when line voltage fluctuates from 90 to 240VAC.
|Heater Element||Platinum/Iridium Filament|
|Pulling Force||(100–400) Solenoid, adjustable|
|Capillary OD Range||1.0–2.0mm|
|Maximum Capillary Length||170mm|
|Minimum Capillary Length||55mm|
|Permanent Memory Set||95|
|Auto Shut-off Time||90 s|
|Power||90–240VAC, 50/60 Hz, Max. 70W|
|Replacement Filaments||13834 2.5mm Square Box Filament, 2.5mm wide|
|DIMENSIONS||30 x 25 x 15 cm (11.75 x 9.8 x 5.9")|
|WEIGHT||20 lb. (9 kg)|
Plautz, C. Z., Williams, H. C., & Grainger, R. M. (2016). Functional Cloning Using a <em>Xenopus</em> Oocyte Expression System. Journal of Visualized Experiments, (107), e53518–e53518. http://doi.org/10.3791/53518
Komarova, Y., Peloquin, J., & Borisy, G. (2011). Components of a microinjection system. Cold Spring Harbor Protocols, 2011(8), 935–9. http://doi.org/10.1101/pdb.ip27
13834 2.5mm Replacement Box Filament