100-Pack of FluoroDish Cell Culture Dish, Coated

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Prices valid in USA, Canada, and PR only.

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Cover-glass bottom for observing and growing cells for imaging

Get the highest quality images and video for your research with FluoroDish Cell Culture dishes.  Their optical quality glass bottom is as thin as a coverslip, which ensures the least amount of distortions and excellent heat transfer without any of the autofluoresence issues so common with plastic petri dishes.  

Choose the style that suits your application. For live cell imaging, embryo research, and life science researchers working with small sample volumes, the 35mm Fluorodish with a 10mm well (FD3510) is ideal. Researchers working with expensive chemicals or experimental drugs choose the FD3510. They are also an excellent choice for microinjection applications, because they are designed with the lowest access angle for easier insertion of a micropipette during cellular microinjection. Fluorodishes are also available in 35mm (FD35) or 50mm (FD5040) sizes for cell culturing applications. For better adhesion of neurons, try the 35mm Fluorodish that is coated with poly-D-lysine (FD35PDL).

Cited in 575 reference articles (in the NIH PubMed Central® alone), our optical grade, glass bottom dishes are unique in the marketplace and conform to strict quality control standards.

 

Prices valid in USA, Canada, and PR only.

See the current Data Sheet.

Options

Order code Description Color
FD35COL-100 FluoroDish Cell Culture Dish, Collagen Coat Clear
FD35FN-100 FluoroDish Cell Culture Dish, Fibronectin Coat Clear
FD35PDL-100 FluoroDish Cell Culture Dish, 35mm Diameter, 23mm Well, Poly-D-Lysine Coat Clear
FD35PLL-100 FluoroDish Cell Culture Dish, Poly-L-Lysine Coat Clear
FD35VN-100 FluoroDish Cell Culture Dish, Vitronectin Coat Clear

Features

  • Optical quality glass bottom for better imaging quality (RI=1.525)
  • Low sample volume for expensive chemicals
  • Lowest access angle for micropipette
  • Qty: 100

 

Improve Research Results

with Fluorodish Optical Grade Glass Bottom Culture Dishes

FluoroDish Cell Culture Dish

Get the highest quality images and video for your research with FluoroDish Cell Culture dishes. Their optical quality glass bottom is as thin as a coverslip, which ensures the least amount of distortions and excellent heat transfer without any of the autofluoresence issues so common with plastic petri dishes.  

Choose the style that suits your application. For live cell imaging, embryo research, and life science researchers working with small sample volumes, the 35mm Fluorodish petri dish with a 10mm well (FD3510) is ideal. Researchers working with expensive chemicals or experimental drugs choose the FD3510. They are also an excellent choice for microinjection applications, because they are designed with the lowest access angle for easier insertion of a micropipette during cellular microinjection. Fluorodishes are also available in 35mm (FD35) or 50mm (FD5040) sizes for cell culturing applications. For better adhesion of neurons, try the 35mm Fluorodish that is coated with poly-D-lysine (FD35PDL).

Cited in 575 reference articles (in the NIH PubMed Central® alone), our optical grade, glass bottom petri dishes are unique in the marketplace and conform to strict quality control standards.

Fluorodish application icons

Optical Grade Glass Bottom for Exceptional Imaging Quality 

The FluoroDish™ bottom glass has superior UV transmission (30% transmission at 300 nm, compared to less than 7% for the most popular German glass). Stringent quality control ensures that glass thickness of the petri dish bottom is 0.17 ±0.01 mm. That is as thin as a coverslip. The thin glass lets you use a higher magnification so you get the highest possible image resolution. Glass avoids the problems of inherent autofluorescence present in plastics and yields a higher signal-to-noise ratio by avoiding interference.

Specially Formulated Low Toxicity Adhesive

FluoroDish™ uses a specially formulated adhesive that is optically clear, durable and with extremely low toxicity. Tests by an independent laboratory have shown that the 96-hour survival rate of rat embryos typically exceeds 95% when kept in a FluoroDish™. 

Benefits

When you need quality glass bottom cell culture dishes with less optical distortion, excellent UV transmission, low cytotoxicity and guaranteed sterility, order WPI Fluorodishes. 

  • Optical quality glass bottom for shorter working distances, larger numerical aperture and higher magnification
  • Non-fluorescent glass so you can discern weaker signals
  • Allows the use of immersion objectives
  • Flat bottom optimizes heat exchange
  • Less optical distortion and superior UV transmission
  • Low cytotoxicity adhesive to ensure cells' survival
  • Individually packaged and gamma sterilized
  • Multiple sizes and designs to suit your application
  • Optional Poly-D-Lysine coating for neurons
  • Dishes designed for low volumes or large growth areas

Applications

  • High resolution image analysis
  • Microinjection
  • Electrophysical recording of fluorescent-tagged cells

WPI’s FluoroDish™ tissue culture dishes provide exceptional imaging quality for many applications requiring the use of inverted microscopes such as high resolution image analysis, microinjection and electrophysical recording of fluorescent-tagged cells. Taking advantage of WPI’s extensive experience with low toxicity adhesives, FluoroDish™ uses a specially formulated adhesive that is optically clear, durable and with extremely low toxicity. Tests by an independent laboratory have shown that the 96-hour surviving rate of embryos is 100% when kept in FluoroDish™ petri dishes, substantially better than other brands. The bottom glass has superior UV transmission (30% transmission at 300 nm, compared to less than 7% for the most popular German glass). Stringent quality control ensures that glass thickness is 0.17 ±0.01 mm.

Conventional plastic petri dishes and chambers limit the use of the inverted scope, because the thick plastic bottom requires a long working distance objective available only in lower magnifications. Each WPI dish has a flat (0.17mm thick), optical quality glass bottom, allowing the use of a much shorter working distance, larger numerical aperture (NA) and higher magnification (up to 100X). The larger NA and higher magnification provide superior quality imaging for both classical and fluorescence microscopy. Higher effective NA yields brighter images for fluorescence and higher resolution in image analysis. The glass bottom permits the use of immersion objectives with media such as water, glycerin or oil for the highest magnification possible. To optimize heat-exchange, WPI’s glass-bottom dish is designed to be flush (flat) with the microscope stage or heating unit, eliminating the air gap that exists with modified plastic dishes where a cover slip was inserted.

Multiple sizes

We have a 50 mm diameter petri dish and two types of 35 mm diameter petri dishes. An inner well is created within the dish by the glass bottom and the tissue culture grade polystyrene which forms the sides of the dish. They are individually packed and gamma sterilized.

The 35 mm petri dish has outside dimensions similar to that of a Corning 35 mm petri dish and has ø23.5 mm glass window (FD35) or ø10 mm glass window (FD3510). Most heaters and perfusion adapters designed for the Corning 35 mm dish also fit this petri dish.

Poly-D-Lysine coating or uncoated

The 23.5 mm glass petri dish is available uncoated or poly-D-lysine-coated. Some cell lines (e.g., PC3 and HEK) adhere well to the uncoated glass bottom petri dish. The poly-D-lysine coating reportedly improves the adhesion of neuron cells. You may also apply any special coating that is best for your cell line to uncoated dishes.

Low volume dishes or large growth areas

The 10 mm glass petri dish (FD3510) has low sidewalls for easy microelectrode access and low solution volume. The low microelectrode access angle is the lowest among all of 35 mm petri dishes on the market (very close to that of a 50 mm dish). The petri dish needs only about 115 µL to cover the bottom well, an important feature when using expensive drugs and chemicals.

The 50 mm petri dish (FD50) has a large growth area (35 mm well diameter), a low access angle for microelectrodes, and grips for easy handling.

Features

  • The 35mm petri dish has outside dimensions similar to that of a Corning 35mm petri dish and has ø23.5 mm glass window. Most heaters and perfusion adapters designed for the Corning 35mm dish will also fit this FluoroDish. Certain types of cell lines, like the PC3 and HEK, adhere well to the uncoated glass bottom petri dish. You can also apply to the uncoated dish any special coating that is best for your cell line.
  • The 50mm cell culture dish has a large growth area (35mm well diameter), a low access angle for microelectrodes, and grips for easy handling.

Choose clear or black cell culture dishes

NOTE: Due to supply constraint, this product is currently non-returnable/non-refundable. For details, please refer to the WPI Terms & Conditions.

More Information
SKU VAR-2827

FluoroDish Certification

Video

Protect Cell Survival and Improve Research Results with WPI Fluorodishes Cell Culture Dishes

 

Standard Fluorodish

Style ID (mm) OD (mm) Glass Ø (mm) Height (inside) Height (outside) Access Angle
FD35 33 35.5 23.5 7.8 9 29°
FD5040 47.5 49.82 35 7.25 7.4 17°

Standard Fluorodish

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Andersen, J. P., Zhang, J., Sun, H., Liu, X., Liu, J., Nie, J., & Shi, Y. (2020). Aster-B coordinates with Arf1 to regulate mitochondrial cholesterol transport. Molecular Metabolism, 42, 101055. https://doi.org/10.1016/j.molmet.2020.101055

Mateus, R., Holtzer, L., Seum, C., Hadjivasiliou, Z., Dubois, M., Jülicher, F., & Gonzalez-Gaitan, M. (2020). BMP Signaling Gradient Scaling in the Zebrafish Pectoral Fin. Cell Reports, 30(12), 4292-4302.e7. https://doi.org/10.1016/j.celrep.2020.03.024

Ibrahim, A. F. M., Shen, L., Tatham, M. H., Dickerson, D., Prescott, A. R., Abidi, N., … Hay, R. T. (2020). Antibody RING-Mediated Destruction of Endogenous Proteins. Molecular Cell, 79(1), 155-166.e9. https://doi.org/10.1016/j.molcel.2020.04.032

Fore, S., Acuña-Hinrichsen, F., Mutlu, K. A., Bartoszek, E. M., Serneels, B., Faturos, N. G., … Yaksi, E. (2020). Functional properties of habenular neurons are determined by developmental stage and sequential neurogenesis. Science Advances, 6(36). https://doi.org/10.1126/sciadv.aaz3173

Alijevic, O., Bignucolo, O., Hichri, E., Peng, Z., Kucera, J. P., & Kellenberger, S. (2020). Slowing of the Time Course of Acidification Decreases the Acid-Sensing Ion Channel 1a Current Amplitude and Modulates Action Potential Firing in Neurons. Frontiers in Cellular Neuroscience, 14, 41. https://doi.org/10.3389/fncel.2020.00041

Van Der Meulen, K. L., Vöcking, O., Weaver, M. L., Meshram, N. N., & Famulski, J. K. (2020). Spatiotemporal Characterization of Anterior Segment Mesenchyme Heterogeneity During Zebrafish Ocular Anterior Segment Development. Frontiers in Cell and Developmental Biology, 8. https://doi.org/10.3389/fcell.2020.00379

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Bolado-Carrancio, A., Rukhlenko, O. S., Nikonova, E., Tsyganov, M. A., Wheeler, A., Garcia-Munoz, A., … Kholodenko, B. N. (2020). Periodic propagating waves coordinate rhogtpase network dynamics at the leading and trailing edges during cell migration. ELife, 9, 1–34. https://doi.org/10.7554/eLife.58165

Ecke, M., Prassler, J., Tanribil, P., Müller-Taubenberger, A., Körber, S., Faix, J., & Gerisch, G. (2020). Formins specify membrane patterns generated by propagating actin waves. Molecular Biology of the Cell, 31(5), 373–385. https://doi.org/10.1091/mbc.E19-08-0460

Mulier, M., Van Ranst, N., Corthout, N., Munck, S., Vanden Berghe, P., Vriens, J., … Moilanen, L. (2020). Upregulation of TRPM3 in nociceptors innervating inflamed tissue. ELife, 9. https://doi.org/10.7554/eLife.61103

Rohani, L., Borys, B. S., Razian, G., Naghsh, P., Liu, S., Johnson, A. A., … Rancourt, D. E. (2020). Stirred suspension bioreactors maintain naïve pluripotency of human pluripotent stem cells. Communications Biology, 3(1). https://doi.org/10.1038/s42003-020-01218-3

Surewicz, W., & Babinchak, W. (2020). Studying Protein Aggregation in the Context of Liquid-liquid Phase Separation Using Fluorescence and Atomic Force Microscopy, Fluorescence and Turbidity Assays, and FRAP. BIO-PROTOCOL, 10(2). https://doi.org/10.21769/bioprotoc.3489

Gao, X., Jiang, Y., Lin, Y., Kim, K. H., Fang, Y., Yi, J., … Tian, B. (2020). Structured silicon for revealing transient and integrated signal transductions in microbial systems. Science Advances, 6(7), 2760. https://doi.org/10.1126/sciadv.aay2760

Shao, W., Yang, J., He, M., Yu, X. Y., Lee, C. H., Yang, Z., … Shi, S. H. (2020). Centrosome anchoring regulates progenitor properties and cortical formation. Nature, 580(7801), 106–112. https://doi.org/10.1038/s41586-020-2139-6

Chronopoulos, A., Thorpe, S. D., Cortes, E., Lachowski, D., Rice, A. J., Mykuliak, V. V., … del Río Hernández, A. E. (2020). Syndecan-4 tunes cell mechanics by activating the kindlin-integrin-RhoA pathway. Nature Materials, 19(6), 669–678. https://doi.org/10.1038/s41563-019-0567-1

Beletkaia, E., Dashtbozorg, B., Jansen, R. G., Ruers, T. J. M., & Offerhaus, H. L. (2020). Nonlinear multispectral imaging for tumor delineation. Journal of Biomedical Optics, 25(09). https://doi.org/10.1117/1.jbo.25.9.096001

Ndao, O., Puech, P. H., Bérard, C., Limozin, L., Rabhi, S., Azas, N., … Dumètre, A. (2020). Dynamics of Toxoplasma gondii Oocyst Phagocytosis by Macrophages. Frontiers in Cellular and Infection Microbiology, 10. https://doi.org/10.3389/fcimb.2020.00207

Otis, J. P., & Farber, S. A. (2016). High-fat Feeding Paradigm for Larval Zebrafish: Feeding, Live Imaging, and Quantification of Food Intake. Journal of Visualized Experiments, (116), e54735–e54735. http://doi.org/10.3791/54735 

Arnold, W. D., Sheth, K. A., Wier, C. G., Kissel, J. T., Burghes, A. H., & Kolb, S. J. (2015). Electrophysiological Motor Unit Number Estimation (MUNE) Measuring Compound Muscle Action Potential (CMAP) in Mouse Hindlimb Muscles. Journal of Visualized Experiments, (103), e52899–e52899. http://doi.org/10.3791/52899 

Gindrat, A.-D., Quairiaux, C., Britz, J., Brunet, D., Lanz, F., Michel, C. M., & Rouiller, E. M. (2015). Whole-scalp EEG mapping of somatosensory evoked potentials in macaque monkeys. Brain Structure & Function, 220(4), 2121–42. http://doi.org/10.1007/s00429-014-0776-y 

Lee, E., Hong, J., Park, Y.-G., Chae, S., Kim, Y., Kim, D., … Sirota, A. (2015). Left brain cortical activity modulates stress effects on social behavior. Scientific Reports, 5, 13342. http://doi.org/10.1038/srep13342 

Nunes, P., Guido, D., & Demaurex, N. (2015). Measuring Phagosome pH by Ratiometric Fluorescence Microscopy. Journal of Visualized Experiments, (106), e53402–e53402. http://doi.org/10.3791/53402 

Pothoven, K. L., Norton, J. E., Hulse, K. E., Suh, L. A., Carter, R. G., Rocci, E., … Schleimer, R. P. (2015). Oncostatin M promotes mucosal epithelial barrier dysfunction, and its expression is increased in patients with eosinophilic mucosal disease. Journal of Allergy and Clinical Immunology, 136(3), 737–746.e4. http://doi.org/10.1016/j.jaci.2015.01.043 

Rees, M. D., & Thomas, S. R. (2015). Using Cell-substrate Impedance and Live Cell Imaging to Measure Real-time Changes in Cellular Adhesion and De-adhesion Induced by Matrix Modification. Journal of Visualized Experiments, (96), e52423–e52423. http://doi.org/10.3791/52423 

Srinivasan, B., Kolli, A. R., Esch, M. B., Abaci, H. E., Shuler, M. L., & Hickman, J. J. (2015). TEER measurement techniques for in vitro barrier model systems. Journal of Laboratory Automation, 20(2), 107–26. http://doi.org/10.1177/2211068214561025 

Steinritz, D., Schmidt, A., Balszuweit, F., Thiermann, H., Ibrahim, M., Bölck, B., & Bloch, W. (2015). Assessment of Endothelial Cell Migration After Exposure to Toxic Chemicals. Journal of Visualized Experiments, (101), e52768–e52768. http://doi.org/10.3791/52768 

Al-Sadi, R., Ye, D., Boivin, M., Guo, S., Hashimi, M., Ereifej, L., … Yaguchi, A. (2014). Interleukin-6 Modulation of Intestinal Epithelial Tight Junction Permeability Is Mediated by JNK Pathway Activation of Claudin-2 Gene. PLoS ONE, 9(3), e85345. http://doi.org/10.1371/journal.pone.0085345 

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Blanchard, E., Zlock, L., Lao, A., Mika, D., Namkung, W., Xie, M., … Richter, W. (2014). Anchored PDE4 regulates chloride conductance in wild-type and ΔF508-CFTR human airway epithelia. FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology, 28(2), 791–801. http://doi.org/10.1096/fj.13-240861 

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Hubbard, D., Ghandehari, H., & Brayden, D. J. (2014). Transepithelial Transport of PAMAM Dendrimers across Isolated Rat Jejunal Mucosae in Ussing Chambers. Biomacromolecules, 15(8), 2889–2895. http://doi.org/10.1021/bm5004465 

Jung, E. S., Park, J., Gee, H. Y., Jung, J., Noh, S. H., Lee, J.-S., … Lee, M. G. (2014). Shank2 mutant mice display a hypersecretory response to cholera toxin. The Journal of Physiology, 592(8), 1809–21. http://doi.org/10.1113/jphysiol.2013.268631 

Ko, E.-A., Jin, B.-J., Namkung, W., Ma, T., Thiagarajah, J. R., & Verkman, A. S. (2014). Chloride channel inhibition by a red wine extract and a synthetic small molecule prevents rotaviral secretory diarrhoea in neonatal mice. Gut, 63(7), 1120–9. http://doi.org/10.1136/gutjnl-2013-305663 

Lomasney, K. W., Cryan, J. F., & Hyland, N. P. (2014). Converging effects of a Bifidobacterium and Lactobacillus probiotic strain on mouse intestinal physiology. AJP: Gastrointestinal and Liver Physiology, 307(2), G241–G247. http://doi.org/10.1152/ajpgi.00401.2013 

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Markov, A. G., Falchuk, E. L., Kruglova, N. M., Rybalchenko, O. V., Fromm, M., & Amasheh, S. (2014). Comparative analysis of theophylline and cholera toxin in rat colon reveals an induction of sealing tight junction proteins. Pflügers Archiv - European Journal of Physiology, 466(11), 2059–2065. http://doi.org/10.1007/s00424-014-1460-z 

Meenach, S. A., Anderson, K. W., Hilt, J. Z., McGarry, R. C., & Mansour, H. M. (2014). High-Performing Dry Powder Inhalers of Paclitaxel DPPC/DPPG Lung Surfactant-Mimic Multifunctional Particles in Lung Cancer: Physicochemical Characterization, In Vitro Aerosol Dispersion, and Cellular Studies. AAPS PharmSciTech, 15(6), 1574–1587. http://doi.org/10.1208/s12249-014-0182-z 

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Perathoner, S., Daane, J. M., Henrion, U., Seebohm, G., Higdon, C. W., Johnson, S. L., … Levin, M. (2014). Bioelectric Signaling Regulates Size in Zebrafish Fins. PLoS Genetics, 10(1), e1004080. http://doi.org/10.1371/journal.pgen.1004080 

San Martín, A., Ceballo, S., Baeza-Lehnert, F., Lerchundi, R., Valdebenito, R., Contreras-Baeza, Y., … Barros, L. F. (2014). Imaging mitochondrial flux in single cells with a FRET sensor for pyruvate. PloS One, 9(1), e85780. http://doi.org/10.1371/journal.pone.0085780 

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Suntornsaratoon, P., Kraidith, K., Teerapornpuntakit, J., Dorkkam, N., Wongdee, K., Krishnamra, N., & Charoenphandhu, N. (2014). Pre-suckling calcium supplementation effectively prevents lactation-induced osteopenia in rats. American Journal of Physiology - Endocrinology and Metabolism, 306(2).

Tradtrantip, L., Ko, E.-A., Verkman, A. S., Walker, C., Rudan, I., Liu, L., … Shen, H. (2014). Antidiarrheal Efficacy and Cellular Mechanisms of a Thai Herbal Remedy. PLoS Neglected Tropical Diseases, 8(2), e2674. http://doi.org/10.1371/journal.pntd.0002674 

Turnbull, L., Strauss, M. P., Liew, A. T. F., Monahan, L. G., Whitchurch, C. B., & Harry, E. J. (2014). Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy (f3D-SIM). Journal of Visualized Experiments, (91), e51469–e51469. http://doi.org/10.3791/51469 

Vajn, K., Suler, D., Plunkett, J. A., Oudega, M., Becker, C., Lieberoth, B., … Umeda, K. (2014). Temporal Profile of Endogenous Anatomical Repair and Functional Recovery following Spinal Cord Injury in Adult Zebrafish. PLoS ONE, 9(8), e105857. http://doi.org/10.1371/journal.pone.0105857 

Wagley, S., Hemsley, C., Thomas, R., Moule, M. G., Vanaporn, M., Andreae, C., … Titball, R. W. (2014). The twin arginine translocation system is essential for aerobic growth and full virulence of Burkholderia thailandensis. Journal of Bacteriology, 196(2), 407–16. http://doi.org/10.1128/JB.01046-13 

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Welling, S. H., Hubálek, F., Jacobsen, J., Brayden, D. J., Rahbek, U. L., & Buckley, S. T. (2014). The role of citric acid in oral peptide and protein formulations: Relationship between calcium chelation and proteolysis inhibition. European Journal of Pharmaceutics and Biopharmaceutics, 86(3), 544–551. http://doi.org/10.1016/j.ejpb.2013.12.017 

Xue, N., Li, X., Bertulli, C., Li, Z., Patharagulpong, A., Sadok, A., & Huang, Y. Y. S. (2014). Rapid patterning of 1-D collagenous topography as an ECM protein fibril platform for image cytometry. PloS One, 9(4), e93590. http://doi.org/10.1371/journal.pone.0093590 

Yao, M., Goult, B. T., Chen, H., Cong, P., Sheetz, M. P., & Yan, J. (2014). Mechanical activation of vinculin binding to talin locks talin in an unfolded conformation. Scientific Reports, 4, 4610. http://doi.org/10.1038/srep04610 

Yusef, Y. R., Thomas, W., & Harvey, B. J. (2014). Estrogen increases ENaC activity via PKCδ signaling in renal cortical collecting duct cells. Physiological Reports, 2(5).

Zhang, J., Jiang, D., & Peng, H.-X. (2014). A pressurized filtration technique for fabricating carbon nanotube buckypaper: Structure, mechanical and conductive properties. Microporous and Mesoporous Materials, 184, 127–133. http://doi.org/10.1016/j.micromeso.2013.10.012 

Zhou, Y., Chu, W., Lei, M., Li, J., Du, W., & Zhao, C. (2014). Application of a continuous intrinsic dissolution–permeation system for relative bioavailability estimation of polymorphic drugs. International Journal of Pharmaceutics, 473(1), 250–258. http://doi.org/10.1016/j.ijpharm.2014.07.012 

Treating diseases mediated by blockade of the epithelial sodium channel with pyrazine-2-carboxamide derivatives. (2014).

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