FluoroDish Cell Culture Dish - 35mm, 23mm well, pkg of 100

Item#: Make Selection

FD35-100, FD35B-100

Cover-glass bottom for observing and growing cells for imaging

  • Optical quality glass bottom for better imaging quality (RI=1.525)
  • Low sample volume for expensive chemicals
  • Lowest access angle for micropipette
  • Black wall available for low background fluorescent measurement
  • Qty: 100
  • Choose color: Clear (FD35-100) or Black (FD35B-100)

Unit Price: varies



For pricing, Customers outside of the US and Canada, please Contact your distributor

See the current Data Sheet.


Part # Color
FD35-100 Clear
FD35B-100 Black

See the current Data Sheet.


  • Multiple sizes and designs to suit your application
  • Optional Poly-D-Lysine coating for neurons
  • Dishes designed for low volumes or large growth areas


  • High resolution image analysis
  • Microinjection
  • Electrophysical recording of fluorescent-tagged cells
  • Black wall available for use with confocal microscopes

WPI’s FluoroDish™ tissue culture dishes provide exceptional imaging quality for many applications requiring the use of inverted microscopes such as high resolution image analysis, microinjection and electrophysical recording of fluorescent-tagged cells. Taking advantage of WPI’s extensive experience with low toxicity adhesives, FluoroDish™ uses a specially formulated adhesive that is optically clear, durable and with extremely low toxicity. Tests by an independent laboratory have shown that the 96-hour surviving rate of embryos is 100% when kept in FluoroDish™, substantially better than other brands. The bottom glass has superior UV transmission (30% transmission at 300 nm, compared to less than 7% for the most popular German glass). Stringent quality control ensures that glass thickness is 0.17 ±0.01 mm.

Conventional plastic dishes and chambers limit the use of the inverted scope, because the thick plastic bottom requires a long working distance objective available only in lower magnifications. Each WPI dish has a flat (0.17mm thick), optical quality glass bottom, allowing the use of a much shorter working distance, larger numerical aperture (NA) and higher magnification (up to 100X). The larger NA and higher magnification provide superior quality imaging for both classical and fluorescence microscopy. Higher effective NA yields brighter images for fluorescence and higher resolution in image analysis. The glass bottom permits the use of immersion objectives with media such as water, glycerin or oil for the highest magnification possible. To optimize heat-exchange, WPI’s glass-bottom dish is designed to be flush (flat) with the microscope stage or heating unit, eliminating the air gap that exists with modified plastic dishes where a cover slip was inserted.

Multiple sizes

We have a 50 mm diameter dish and two types of 35 mm diameter dishes. An inner well is created within the dish by the glass bottom and the tissue culture grade polystyrene which forms the sides of the dish. They are individually packed and gamma sterilized.

The 35 mm dish has outside dimensions similar to that of a Corning 35 mm dish and has ø23.5 mm glass window (FD35) or ø10 mm glass window (FD3510). Most heaters and perfusion adapters designed for the Corning 35 mm dish also fit this dish.

Poly-D-Lysine coating or uncoated

The 23.5 mm glass dish is available uncoated or poly-D-lysine-coated. Some cell lines (e.g., PC3 and HEK) adhere well to the uncoated glass bottom dish. The poly-D-lysine coating reportedly improves the adhesion of neuron cells. You may also apply any special coating that is best for your cell line to uncoated dishes.

Low volume dishes or large growth areas

The 10 mm glass dish (FD3510) has low sidewalls for easy microelectrode access and low solution volume. The low microelectrode access angle is the lowest among all of 35 mm dishes on the market (very close to that of a 50 mm dish). The dish needs only about 115 µL to cover the bottom well, an important feature when using expensive drugs and chemicals.

The 50 mm dish (FD50) has a large growth area (35 mm well diameter), a low access angle for microelectrodes, and grips for easy handling.

FluoroDish Cell Culture Dish Measurements

Measurements and specifications of the FD35 cell culture dish.

ID: 33 mm
OD: 35.5 mm
Glass Diameter Φ: 23.5 mm
height (inside): 7.8 mm
Height (outside): 9 mm
Access angle: 29°


Choose clear or black cell culture dishes


The 35mm dish has outside dimensions similar to that of a Corning 35mm dish and has ø23.5 mm glass window. Most heaters and perfusion adapters designed for the Corning 35mm dish will also fit this FluoroDish. Certain types of cell lines, like the PC3 and HEK, adhere well to the uncoated glass bottom dish. You can also apply to the uncoated dish any special coating that is best for your cell line.

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