WPI's new generation pressure microinjectors are quickly replacing the very popular legacy line of pnuematic valve PicoPumps Pressure Injectors. The PV820 and PV830 have been a staple in the microinjection market for decades. The three new injectors facilitate any type of microinjecion that involves glass micropipettes.

• MICRO-ePUMP - Microinjector with Integrated Cell Penetrator and Internal Pressure Source
• μPUMP - Microinjector with Internal Pressure Source
• PV850 - Microinjector for Use with External Pressure Source 

One common microinjection method uses a glass micropipette to inject a liquid substance into a living cell or into the intercellular space, typically using an inverted microscope. The glass microinjection needle tip can vary in size from sub-micron to about 5-6µm in diameter. It will be larger if you are injecting stem cells into an embryo. The needles penetrate the cell membrane or even the nuclear envelope, in order to introduce a vector into a cell or nucleus. The vector could be a DNA molecule that carries foreign genetic material into the host cell where it replicates. By altering the embryo, microinjection can be used to genetically modify various animals like mice, fish (zebrafish, medaka, etc.), C. elegans, rats, rabbits, and even large domesitcated animals  like cattle, sheep and pigs.

 

Features

	MICRO-ePUMP	uPUMP	PV850
Regulated compensation and injection pressure
Pressure output: 0.3–87 PSI
Low volume tubing assembly
Injection using foot switch (included)
Injection using a TTL pulse from external computer or pulse generator (Cable to connect to pulse generator included)
Manual injection using touch screen interface
Integrated pressure source
2-Stage foot switch to activate voltage for injection and MICRO-ePORETM
Onboard MICRO-ePORETM (The Pinpoint Cell Penetrator for targeted microinjection and increased viability of injected embryos has an audio continuity tone indicating active probe, an injection counter to indicate total number of injections, and user-adjustable frequency and voltage through a touch screen.)

 

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Benefits 

• Convenient internal pressure source (MICRO-ePUMP and uPUMP only)
• Intuitive user-interface
• Small footprint takes up very little bench space
• Easy to navigate with touch screen and control knob
• High precision for reproducible injections – microliters to picoliter
• All inclusive system 
• Plug-and-play system to begin working as quickly as possible
• Compensation pressure comparisson for accuracy 

  

Applications

• Explosive sensing with insect-based biorobots
• Electron transfer kinetics on mono and multilayer graphene
• Creation of transgenic animals
  • Any nucleic acid can be introduced into any kind of cell
  • No length limitation (100’s of kb’s of DNA) on the transfer of foreign genes
  • Used to genetically modify animals like mice, fish, C. elegans, rats, rabbits and large domestic animals such as cattle, sheep, and pigs
    
    
    
    
• Injecting substances into cells
  • Nucleic acids
  • Organelles
  • Kinases
  • Histochemical markers (such as horseradish peroxidase or fluorescent yellow)
  • Proteins, antibodies
  • Metabolites
  • Ions
• Microperfusion, which is the precise delivery of a small amount (pL to µL) of drug to a single cell or group of cells, such as a pharmacological drug test
• Analysis of early development processes like gastrulation, organogenesis, etc.  

 

All Your Microinjection Accessories

Let WPI be your one-stop shop for all your microinjection needs. From microscopes and manipulators to pre-pulled glass pipettes, WPI offers many options for your microinjection system. 

TAKE A LOOK AT OUR MICROINJECTION ONE-STOP-SHOP!

 

Who Is WPI?

With over 50 years in the business of serving scientists and researchers, you can trust us.

 

* This offer is valid on USA orders only.

Testimonials

I recently purchased WPI’s New STX2-PLUS Electrode with 99675 adapter to use with EVOM2 and measured in 24-well Falcon individual inserts for my experiments. Oh boy, it worked fantastic! I was so amazed how easy handling and great stability on measurements!!!! I have to measure 51 wells + controls for each experiments. I cannot thank you enough for WPI and you (Jim) for developing such a fantastic probe with affordable price!!!!! May 29, 2020

 

The WPI Nanoliter 2010 with SMARTouch controller is a great step forward from the previous model Nanoliter 2000, providing easier handling, an improved sealing gasket for the micropipette, and much easier control of injection volumes and stepless injection rate. We use it for stereotaxic viral vector and tracer injections into the mouse brain and have found that it gives excellent results with no spillage at the entry site. In addition, WPI’s customer service has been very attentive and helpful. Definitely a five-out-of-five-star rating. Dr. Quirin Krabichler Institute of Anatomy and Cell Biology, Universität Heidelberg, Germany.  September 30, 2019

 

Found your website via Google, setting up the account was super fast and easy. Found what i was looking for and buying it was a breeze. I ordered one to test it, loved it so much i ordered a second one.  Cary & Tanya Markham

 

Thank you, Cathy! I think your company representatives and customer care is terrific. When I called to make changes and update the quote and Matt Frasure was unavailable, Tammy Villadoniga stepped up and took care of what we needed to move this the quote along. Then, Larry Burns, took great care with processing the order itself and you have just finalized things with the email notification of charges and an invoice. The speed, efficiency and friendly factor is outstanding. Tracey Purcell University of Arizona

 

Jessica, THAT'S an excellent solution! If that works for your end, great! And, THANK YOU SO MUCH!! WOW! What excellent customer service!  Victoria Sample Missouri Western State University