FluoroDish Cell Culture Dish - 35mm, 10mm well, pkg of 100

Order code

Cover-glass bottom for observing and growing cells for imaging

  • Optical quality glass bottom for better imaging quality (RI=1.525)
  • Low sample volume for expensive chemicals
  • Lowest access angle for micropipette
  • Qty: 100


Improve Research Results

with Fluorodish Optical Grade Glass Bottom Culture Dishes

FluoroDish Cell Culture Dish

Get the highest quality images and video for your research with FluoroDish Cell Culture dishes.  Their optical quality glass bottom is as thin as a coverslip, which ensures the least amount of distortions and excellent heat transfer without any of the autofluoresence issues so common with plastic dishes.  

Choose the style that suits your application. For live cell imaging, embryo research, and life science researchers working with small sample volumes, the 35mm Fluorodish with a 10mm well (FD3510) is ideal. Researchers working with expensive chemicals or experimental drugs choose the FD3510. They are also an excellent choice for microinjection applications, because they are designed with the lowest access angle for easier insertion of a micropipette during cellular microinjection. Fluorodishes are also available in 35mm (FD35) or 50mm (FD5040) sizes for cell culturing applications. For better adhesion of neurons, try the 35mm Fluorodish that is coated with poly-D-lysine (FD35PDL).

Cited in 575 reference articles (in the NIH PubMed Central® alone), our optical grade, glass bottom dishes are unique in the marketplace and conform to strict quality control standards.

  Fluorodish application icons



When you need quality glass bottom cell culture dishes with less optical distortion, excellent UV transmission, low cytotoxicity and guaranteed sterility, order WPI Fluorodishes. 

  • Optical quality glass bottom for shorter working distances, larger numerical aperture and higher magnification
  • Non-fluorescent glass so you can discern weaker signals
  • Allows the use of immersion objectives
  • Flat bottom optimizes heat exchange
  • Less optical distortion and superior UV transmission
  • Low cytotoxicity adhesive to ensure cells' survival
  • Individually packaged and gamma sterilized
  • Multiple sizes and designs to suit your application
  • Optional Poly-D-Lysine coating for neurons
  • Dishes designed for low volumes or large growth areas


Optical Grade Glass Bottom for Exceptional Imaging Quality 

The FluoroDish™ bottom glass has superior UV transmission (30% transmission at 300 nm, compared to less than 7% for the most popular German glass). Stringent quality control ensures that glass thickness of the dish bottom is 0.17 ±0.01 mm. That is as thin as a coverslip. The thin glass lets you use a higher magnification so you get the highest possible image resolution. Glass avoids the problems of inherent autofluorescence present in plastics and yields a higher signal-to-noise ratio by avoiding interference.


Specially Formulated Low Toxicity Adhesive

FluoroDish™ uses a specially formulated adhesive that is optically clear, durable and with extremely low toxicity. Tests by an independent laboratory have shown that the 96-hour survival rate of rat embryos typically exceeds 95% when kept in a FluoroDish™. 


See the current Data Sheet.


  • Optical quality glass bottom for shorter working distances, larger numerical aperture and higher magnification
  • Non-fluorescent glass so you can discern weaker signals
  • Allows the use of immersion objectives
  • Flat bottom optimizes heat exchange
  • Less optical distortion and superior UV transmission
  • Low cytotoxicity adhesive to ensure cells' survival
  • Individually packaged and gamma sterilized
  • Multiple sizes and designs to suit your application
  • Optional Poly-D-Lysine coating for neurons
  • Dishes designed for low volumes or large growth areas


  • High resolution image analysis
  • Microinjection
  • Electrophysical recording of fluorescent-tagged cells

WPI’s FluoroDish™ tissue culture dishes provide exceptional imaging quality for many applications requiring the use of inverted microscopes such as high resolution image analysis, microinjection and electrophysical recording of fluorescent-tagged cells. Taking advantage of WPI’s extensive experience with low toxicity adhesives, FluoroDish™ uses a specially formulated adhesive that is optically clear, durable and with extremely low toxicity. Tests by an independent laboratory have shown that the 96-hour surviving rate of embryos is 100% when kept in FluoroDish™, substantially better than other brands. The bottom glass has superior UV transmission (30% transmission at 300 nm, compared to less than 7% for the most popular German glass). Stringent quality control ensures that glass thickness is 0.17 ±0.01 mm.

Conventional plastic dishes and chambers limit the use of the inverted scope, because the thick plastic bottom requires a long working distance objective available only in lower magnifications. Each WPI dish has a flat (0.17mm thick), optical quality glass bottom, allowing the use of a much shorter working distance, larger numerical aperture (NA) and higher magnification (up to 100X). The larger NA and higher magnification provide superior quality imaging for both classical and fluorescence microscopy. Higher effective NA yields brighter images for fluorescence and higher resolution in image analysis. The glass bottom permits the use of immersion objectives with media such as water, glycerin or oil for the highest magnification possible. To optimize heat-exchange, WPI’s glass-bottom dish is designed to be flush (flat) with the microscope stage or heating unit, eliminating the air gap that exists with modified plastic dishes where a cover slip was inserted.

Multiple sizes

We have a 50 mm diameter dish and two types of 35 mm diameter dishes. An inner well is created within the dish by the glass bottom and the tissue culture grade polystyrene which forms the sides of the dish. They are individually packed and gamma sterilized.

The 35 mm dish has outside dimensions similar to that of a Corning 35 mm dish and has ø23.5 mm glass window (FD35) or ø10 mm glass window (FD3510). Most heaters and perfusion adapters designed for the Corning 35 mm dish also fit this dish.

Poly-D-Lysine coating or uncoated

The 23.5 mm glass dish is available uncoated or poly-D-lysine-coated. Some cell lines (e.g., PC3 and HEK) adhere well to the uncoated glass bottom dish. The poly-D-lysine coating reportedly improves the adhesion of neuron cells. You may also apply any special coating that is best for your cell line to uncoated dishes.

Low volume dishes or large growth areas

The 10 mm glass dish (FD3510) has low sidewalls for easy microelectrode access and low solution volume. The low microelectrode access angle is the lowest among all of 35 mm dishes on the market (very close to that of a 50 mm dish). The dish needs only about 115 µL to cover the bottom well, an important feature when using expensive drugs and chemicals.

The 50 mm dish (FD50) has a large growth area (35 mm well diameter), a low access angle for microelectrodes, and grips for easy handling.


The 35mm dish has outside dimensions similar to that of a Corning 35mm dish and has ø23.5 mm glass window. Most heaters and perfusion adapters designed for the Corning 35mm dish will also fit this FluoroDish. Certain types of cell lines, like the PC3 and HEK, adhere well to the uncoated glass bottom dish. You can also apply to the uncoated dish any special coating that is best for your cell line.


FluoroDish Cell Culture Dish Measurements

This image shows the measurements of the FD3510 cell culture dish.

Measurements and specifications of the FD3510 cell culture dish

NOTE: 17º angle of incline on the dish.


Style ID (mm) OD (mm) Glass Ø (mm) Height (inside) Height (outside) Access Angle
FD35 33 35.5 23.5 7.8 9 29°
FD5040 47.5 49.82 35 7.25 7.4 17°

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