Epithelial Physiology

Epithelial Physiology

During the last 30 years, TEER measurements have become universally established as the most convenient, reliable and non-destructive method of evaluating and monitoring the growth of epithelial tissue culture in vitro. The confluence of the cellular monolayer is quickly determined by a sharp increase in TEER. TEER measurement technology, first introduced to tissue and cell biologist by WPI in the mid '80s has since been perfected and expanded to include a range of TEER related manual and automatic instrumentation.

For epithelial physiology studies, our Ussing systems offer a quick, effective means of making low-resistance electrical connections to the Ussing chamber without the need of long agar bridges. Ag/AgCl half-cells screw into short tubes which plug firmly into place in the chamber's luer ports, eliminating the inconvenience and expense of Calomel half-cells in open liquids.

  1. Epithelial Voltage Clamp
    You can monitor membrane permeability as a function of membrane voltage or applied chemicals using our epithelial voltage clamp (EVC) system. Using this system, you can efficiently clamp either current or voltage on up to 4 sample membranes simultaneously.
  2. TEER Measurement
    In epithelial cell research, there are different types of cells which are typically cultured. We will focus on epithelial cells. Epithelial tissue lines the surface and cavities of an organ. Epithelium includes your skin tissue, but it also lines the alimentary canal, organs and blood vessels. Epithelial cell cultures may be used for drug discovery or other applications that explore the transport or diffusion of a substance through an epithelial monolayer. Such a tissue research study requires a confluent monolayer that completely covers the culture substrate it is grown on. 
  3. Ussing System
    The Ussing system for cell biology research was designed for studying the transport of ions, nutrients and drugs across a whole barrier tissue, like epithelial tissues. The Ussing system consists of the Ussing chamber to hold the tissue and a perfusion system. WPI's Ussing systems were developed for electrophysiology applications. A complete Ussing system would also include a WPI amplifier and data acquisition equipment. Our Ussing systems offer a quick, effective means of making low-resistance electrical connections to the Ussing chamber without the need of long agar bridges. Ag/AgCl half-cells screw into short tubes which plug firmly into place in the chamber's luer ports.   
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