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  2. Versatile Microinjection Syringe Pump (UMP3T) for Injections in the Nanoliter to Milliliter Range
    May 15, 2020
    The UltraMicroPump3 is a versatile microinjection syringe pump designed to support the needs of a busy lab. The UMP3 Microinjector is suitable for delivery of media from nanoliters to milliliters. the UMP3 injecotor’s user interface and system design enables delivery of highly accurate and repeatable injections. The choice for thousands of scientists, it is widely cited in research papers.   Intuitive Touchscreen Display for Easy Setup The SMARTouch controller has an intuitive touch screen interface and an easy-to-use menu for quick configuration of the syringe size, volumes and flow rates. The total system calibration is designed to eliminate human errors and reduce experimental processing time. Configuration can be saved and subsequently imported as a script via the USB port. Control 2 Injectors Simultaneously The SMARTouch controller can independently control two microinjection syringe pumps (microinjectors) simultaneously, providing complete freedom in the experimental procedures. You can trigger the injection directly on the touchscreen or using the optional footswitch to achieve a “hands-free” start and stop operation. Feature-Rich Graphic Interface Thanks to the color touchscreen, researchers get vital feedback during the experiments. You can follow the injections with a graphical indication of the flow and the volume remaining in each syringe at any time. Other views offer a range of alternate information screens to suit experimental needs. User-Configurable Mounting Bar Depending on yo
  3. Why Choose an EVOM3 Over an EVOM2 for TEER Measurement
    May 15, 2020
    After 12 years, the next generation of the EVOM is born. Used for measuring Transepithelial Electrical Resistance (TEER) and monitoring the confluence of cell monolayers in culture well plates the EVOM3 TEER Measurement System enables researchers to carry out experiments more efficiently by improving the workflow, and increasing the stability and accuracy of readings over that of the EVOM2.  Here are six of the EVOM3 improvements.    
  4. 7 Reasons to Love the New EVOM3 for TEER Measurement
    May 15, 2020
    The NEW EVOM3 Epithelial Volt/Ohm Meter delivers improved workflow efficiency, increased stability and more repeatable measurements than traditional Trans Epithelial Electrical Resistance (TEER) meters. Here are seven things you will love about the new EVOM3.     1. It has an intuitive Touchscreen Display Providing users with vital feedback during experimental measurements, the EVOM3’s large touch screen offers a range of informational views. The new graphical displays for trend analysis and measurement values helps you deliver a simple, stepwise methodology during experimental measurements. The touch screen interface has an intuitive, easy-to-use menu for configuration and calibration that is designed to eliminate human errors and reduce experimental processing time. 2. EVOM3 offers Auto Data Logging Eliminating the need to log data by hand, the EVOM3 writes the resistance or voltage information to a USB drive in CSV format for easy transfer to spreadsheets and data analysis programs. 3. With the EVOM3 you get Hands Free Recording When used with the footswitch, it enables hands-free recording of measurements. 4. You can make Quick, Easy, Reliable Readings At the heart of the EVOM3 is our latest processor and circuitry, providing users with quick, easy and reliable readings due to its fast stabilization, automatic twenty times sampling average and low noise d
  5. Why Choose a Reusable Face Mask?
    April 23, 2020
    WPI offers the S9 self-priming respirator, a critical component of your personal protective equipment (PPE). The S9 is a reusable industrial face mask designed to KN95 standards. This means that it will block >95% of all particulate matter in the air larger than 2 µm in size. The face mask employs inexpensive replaceable filters. Looking at the initial cost, you may wonder if the resusable mask is worth the higher cost. This mask pays for itself very quickly when you consider how many disposable masks it replaces. Reuseable Face Mask Lasts Longer The long length of service of a WPI industrial reusable face mask (as opposed to disposable masks that use the same sort of filter material) is the result of its design. A plastic filter shield is securely attached to a silicone face mask. Together the face mask and filter shield can last up to two (2) years. The plastic filter shield houses the actual filter cartridge, which is disposable. With a disposable mask, people are constantly readjusting the mask. Their hands are usually what transfers pathogens from the surfaces they touch to their faces, noses and mouths. That’s why the CDC advises that we constantly wash our hands and not touch our faces. Each time we adjust a disposable mask with our hands, we contaminate it. The outside of every disposable mask should be considered "dirty." It is likely contaminated. In the case of WPI's reusable face mask, the filter is protected from our hands by a plastic housing which does not obstruct airflow. The mask is easily taken on and off without directly touching the filters. In a normal environment, the filters should be replaced every seven (7) to ten (10) days. In high risk environments, change the filters every three (3) to five (5) days.  To clean the face mask, simply wipe it with hot water (75°C) or alcohol. Watch the video below to see how easy it is to replace the filters in the reusable face mask.   Save Money with Reusable Mask Let's compare WPI's reusable face mask with typical disposable face masks. In a normal environment, you should change a disposable mask twice a day. WPI's face mask with a s
  6. Significance of Transepithelial/ Transendothelial Electrical Resistance (TEER) Measurement in Lung In vitro Models 
    April 13, 2020
      Epithelial and endothelial cells are known for their barrier function like selective permeability. Lung tissue is comprised of epithelial cellular layers, residing adjacent to endothelial layers and allowing exchange of oxygen and carbon dioxide between lung and blood. Currently, the biomedical research community’s major focus area is to understand the details of the infection caused by this novel coronavirus (2019-nCoV) or by similar viruses. This new virus can disrupt the lung’s normal cleaning ability to get rid of foreign particles in the lung, and the selective permeability or barrier function of the lung may become severely impaired. The adverse physiological effect may be sustained once the inflammatory cascade is initiated. Researchers in collaboration with clinicians are trying to develop vaccines and drugs to
  7. 8 Good Reasons to Choose the MICRO-ePUMP Microinjector for your Applications
    April 10, 2020
    Do you need a precision microinjector with an integrated pressure source for injections in the picoliter range? WPI’s new MICRO-ePUMP, which is comparable to the Ependorf Femtojet, is a portable solution for injecting DNA, RNA and proteins, as well as pre- and post- implantation in embryos. Whether you are doing disease modeling with zebrafish or viral research for SARS-CoV2, the MICRO-ePUMP has much to offer. Here are eight good reasons to choose the MICRO-ePUMP for your applications.   Convenient internal pressure source The convenient internal pressure source i
  8. Genetic Transfer (viral or non-viral vector) Using Microprocessor-Controlled Injector
    March 02, 2020
    With the first approved human gene therapy trial in 19891 (Rosenberg, et.al.), gene therapy has come a long way in modern medicine and is making inroads in clinics and the market in general.2,3 2017 was an important year for gene therapy when Luxurna, the first human gene therapy drug for an inherited retinal dystrophy, was approved by Food and Drug Administration (USA).4 Now several drugs are undergoing clinical trials. With an estimated $11 billion (USD) market in the next 10 years, both clinical trials and the pharmaceutical industry are expected to benefit immensely from gene therapy.5 With improved use of viral vectors (Adeno-associated virus (AAV), adenovirus, lentivirus, retrovirus, HSV) or non-viral vectors, research and clinical trials on the development of therapeutic genes have witnessed great
  9. Benefits of Nitric Oxide Detection using the WPI Free Radical Analyzer
    February 25, 2020
    Nitric oxide (NO) is an essential signaling molecule and is known to play a significant role in a multitude of physiological systems including the central nervous system (CNS), the cardiovascular system, the gastrointestinal tract, the immune system, and the renal system. 1-5 However, being highly reactive, detection and quantification of NO is very difficult.6,7 It requires a sensor that is sensitive, selective to NO, and easy to calibrate. WPI’s Free Radical Analyzer (4-channel TBR4100  and single-channel TBR1025) and the LabTrax Data Acquisition System with the options of using nano NO sensors, micro NO sensors, flexible micro sensors, and macro nitric oxide sensors enable amperometric (electrical based) detection of NO dissolved in liquids.  Features & Benefits of the Free Radical Analyzer and Nitric Oxide Detection System Rapid response time: < 5 sec enhances detection capability of the highly reactive, short-lived NO molecules High sensitivity: ≤2 pA/nM – enables detection of low levels of NO Excellent selectivity to NO: NaNO2 (10-6 or better) prevents contamination with other isoforms Nano and microsensors & macrosensors are available: Nano and microsensors for measurement of tiny volume or space application Macrosensors with permeable sleeve to prevent protein deposition and interference from other constituents Every sensor is quality tested and sold with a calibration certificate Simple and easy calibration and detection method User friendly software for data recording (Labtrax) Numerous citations in the literature for detection in biological tissues, cell cultures and liquids. Tips for Efficient and Accurate Detection Make fresh calibration solutions. Calibration solution, such as SNAP can be stored at 4°C for 1 week away from light (covered in aluminum foil). When precise detection is critical, perform daily calibrations before measuring unknown samples. Changes in temperature and pH are known to affect the electrical read outs. Therefore, calibration should be performed at the same temperature and pH as the sample. Follow the long-term and short-term storage instruction for maintaining functional longevity of the sensor. All the sensors have a detection range (concentration) posted in the website. Determine from literature the expected concentration of nitric oxide in your sample (e.g., produced in cell culture by specific cell type) then select the sensor that can detect the lowest concentration. If the sample concentration is high, it is possible to bring it within the detection range by diluting the sample. WPI’s Free Radical Analyzer (4-channel TBR4100 and single-channel TBR1025) and the LabTrax Data Acquisition System can also be used for detection of other free radicals, including pH, oxygen (O2), hydrogen peroxide (H2O2), carbon dioxide (CO2), and hydrogen sulfide (H2S). For more information contact WPI Technical Support or one of our Field Applications Specialist.     More Info   References Calabrese, V.; Mancuso, C.; Calvani, M.; Rizzarelli, E.; Butterfield, D.A.; Giuffrida Stella, A.M. Nitric oxide in the central nervous system: Neuroprotection versus neurotoxicity. Nat. Rev. Neurosci. 2007, 8, 766.  Loscalzo, J.; Welch, G. Nitric oxide and its role in the cardiovascular system. Prog. Cardiovasc. Dis. 1995, 38,87–104. Lanas, A. Role of nitric oxide in the gastrointestinal tract. Arthritis Res. Ther. 2008, 10, S4.  Bogdan, C. Nitric oxide and the immune response. Nat. Immunol. 2001, 2, 907–916.  Mount, P.F.; Power, D.A. Nitric oxide in the kidney: Functions and regulation of synthesis. Acta Physiol. 2006,187, 433–446. Butler, A.R.; Flitney, F.W.; Williams, D.L.H. NO, nitrosonium ions, nitroxide ions, nitrosothiols and iron-nitrosyls in biology: A chemist’s perspective. Trends Pharmacol. Sci. 1995, 16, 18–22. Gaston, B. Nitric oxide and thiol groups. Biochim. Biophys. Acta Bioenerg. 1999, 1411, 323–333. 
  10. New Technology Makes Microinjection More Reliable, Repeatable and Affordable
    January 24, 2020
    Microinjection is the process of transferring genetic materials into a living cell using glass micropipettes or metal microinjection needles. Glass micropipettes can be of various sizes with tip diameters ranging from 0.1 to 10 µm. DNA or RNA is injected directly into the cell’s nucleus. Microinjection has been successfully used with large frog eggs, mammalian cells, mammalian embryos, plants and tissues. Microinjection has been expensive, can be a slow process and requires skilled personnel, but new technologies are making it even more reliable, repeatable and affordable. Pronuclear injection, inserting DNA or RNA into the nucleus of a fertilized oocyte to create transgenic organisms, lets researchers study the role of particular genes. This horizontal gene transfer can insert genetic material from the same species or a different one. When using genetic material from different species, a chimera is created. If the genetic material is NOT integrated into the genome of the offspring, it is considered transient transformation and is not passed to subsequent generations. However, if the new genetic material is transferred to future generations, then we have a stable transformation. In this case, the gene inserted by microinjection is called as the transgene, and the organism that develops after a successful gene transfer is called as transgenic. So, transgenic animals are the results of experimentation that integrates genetic material (DNA) into their germ line. These transgenic animals are invaluable in the quest to identify the functions of specific factors in homeostatic systems through the over-expression or under-expression of a modified gene. In many cases transgenic animals are dependent on the laboratory environment for survival. Microinjection is a highly reproducible and repeatable method of introducing genetic material into a nucleus. (Qingsong Xu. Micromachines for Biological Micromanipulation. Springer Publication, 2018.) Compared with other methods of genetic manipulation, microinjection optimized the materials used and eliminates a lot of waste. (Chow YT, Chen S, Wang R, et al. Single Cell Transfection through Precise Microinjection with Quantitatively Controlled Injection Volumes. Sci Rep. 2016;6:24127. Published 2016 Apr 12. doi:10.1038/srep24127.) Because the materials used are minimized, there is also a reduction in the cost of the materials needed. With the tight control of the microinjection process, the researcher can obtain the precise integration of the recombinant gene in a limited number of copies. Products for Efficient Microinjection WPI offers a broad range of laboratory equipment used for microinjection applications. Our injection systems have been serving scientist for over 30 years. Additionally, WPI offers a variety of accessories for microinjection including pumps, pullers, pipetters, microscopes and more. One of the most popular pumps for zebrafish and adherent cell microinjection is the PV820 Pneumatic PicoPump. Injection Pumps The PV820 and PV830, Pneumatic PicoPumps, were designed to simplify intracellular injection. Researchers get repeatable microinjections in volumes ranging from picoliters to nanoliters. Both of our pneumatic pumps offer eject and hold pressure. To guard against injectant dilution, the hold pressure prevents backfilling of the pipette via capillary action, keeping the injectant meniscus at the pipette tip. In addition, the PV830 also has vacuum pressure, which ensures a secure hold on a Xenopus embryo or other disassociated cell with a vacuum pipette while you inject using the pressure pipette. WPI also has popular pumps for injecting in the picoliter and nanoliter ranges. Read More… Pinpoint Cell Penetrator The new WPI MICRO-ePORE™ pinpoint cell penetrator is a simple and versatile system that can be used for efficient microinjection of a diverse array of compounds and biomolecules into oocytes and pre-implantation stage mammalian embryos. Patent pending Flutter Electrode Technology assists in small, clean, precise membrane penetration without tearing or damaging the membrane when one is working on the microinjection of transgenic animals or cell manipulation. WPI's MICRO-ePORE™ Pinpoint Cell Penetrator offers several advantages over traditional electroporation for the purpose of microinjection. The pinpoint cell penetrator uses a much lower voltage to open a port into the cell membrane. Electroporation is a shotgun approach that opens many pores in the cell membrane. In contrast, the pinpoint cell penetrator targets a specific area of the cell membrane at the very point of microinjection. Survivability of embryos is significantly higher when using pinpoint cell penetration versus electroporation. Read More… Microscope WPI's PZMIII-MI Microscope with Illuminated Base and Articulating Mirror is perfect for microinjection and transfection. It includes a standard stereo microscope head mounted on a research grade Brightfield/Darkfield pole type stand. It has a large stable work surface and a rotatable lens/mirror system which provides transmitted LED intensity illumination. The sliding mirror is gimbaled, allowing for a full range of movement front to back, as well as rotation. The mirror rotates 360º on one axis and can slide for further lighting effect directionally, front to back. A knob on the right of the base adjusts the mirror and a locking ring holds the desired mirror position. Vary the microscope illumination from Brightfield LED to Darkfield LED at an appropriate angle using the articulating mirror. It is an effective tool for viewing live bacteria. At low magnifications, view tissues, cells or embryo transfer where oblique, transmitted illumination is critical. Read More… Get More Information Microinjection is a revolutionary tool in the age of modern science. These methods allow any lab to incorporate the technique of microinjection into their experimental repertoire. Whether DNA, RNA or protein is the molecule of interest, microinjection provides a means of studying function within the context of the living cell. The technology is remarkably accessible and relatively inexpensive, while the possibilities are virtually endless.   More Info
  11. Advanced Flowcell Cleaning for Liquid Waveguide Capillary Cells
    December 20, 2019
    The purpose of this article is to describe a new cleaning procedure for thoroughly cleaning WPI flowcells, including Liquid Waveguide Capillary Cells (LWCCs), UltraPath flowcells and optical cuvettes. The image (right) shows a 3000 series LWCC with a MiniStar pump and the LWCC injection system placed on top of it. Preparation of Chemicals All chemical reagents should be of at least ACS-Grade, preferably HPLC-Grade. This procedure involves the use of caustic and flammable reagents. Consult the manufacturer’s MSDS for necessary safety precautions. Cleaning Solution #1: 0.5M Potassium Hydroxide in 100% Ethanol (e.g.: 7 g KOH in 250mL EtOH). After thoroughly mixing, filter the solution through a 20µm pore size filter. Cleaning Solution #2: 100% Methanol, HPLC grade Cleaning Solution #3: Ultrapure water, Type I per ASTM D1193-99 or equivalent NOTE: Grade 1 ultrapure water per ISO 3696 differs significantly from the above classification. Cleaning Procedure The preferred method of cleaning involves the use of a spectrometer (e.g. Tidas E Base) or photometer based detection system in “monitor mode” throughout the entire cleaning process. This allows the technician to observe the extent of performance improvement as a function of time. The image (right) shows the top view of a MiniStar pump and an LWCC Injection System. The injection system has the DIW and Waste containers at the back, and the three cleaning solutions and a sample bottle on the right side. The simplest cleaning method involves using a peristaltic pump (#MiniStar) to flow each cleaning solution through the sample cell in the appropriate order. A convenient way to perform this task is to use WPI’s LWCC Injection System (#89372). It is recommended that the pump be configured to “pull” through the cell to avoid possib
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