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Laboratory Equipment Blog

  1. Surgical Instrument Care and Handling
    June 19, 2015
    The proper care and handling of your valuable surgical instruments will improve their longevity and function. Choose a protocol appropriate for your environment from the cleaning techniques below. See the videos here. RINSING Immediately after use, rinse instruments under warm or cool running water to remove all blood, body fluids and tissue. Dried soils may damage the instrument surface and make cleaning very difficult. Do not use hot water as this will coagulate proteinous substances. CLEANING TECHNIQUES Time, temperature, and agitation play important roles in the cleaning process. Time — the efficiency of cleaning chemicals is often time dependant Temperature — higher temperature cleaning solutions result in better cleaning Agitation — whether manual or ultrasonic, it is helpful in loo
  2. When a Bigger Bolus is Better
    June 12, 2015
    First, let's consider volumes. A milliliter (mL) is one thousandth the volume of a liter (L) or 10-3L A microliter (µL) is one thousandth the volume of a mL (10-6L) A nanoliter (nL) is one thousandth the volume of a µL (10-9L) A picoliter (pL) is one thousandth the volume of a nL (10-12L) This is graphically represented at the right. Notice that the mL is one trillion times larger than the picoliter. The table (right) shows that the side of a cube with a volume of 1mL is 1cm long. Likewise, the side of a cube with a volume of 1pL is 10µm long. Just for comparison, it shows that the diameter of a sphere with a volume of 1mL is 1.24cm, and the volume of a sphere with a volume of 1pL is 12.4µm. With these volume comparisons in
  3. Born Before the Test Tube Baby
    May 14, 2015
    One of WPI's articles was posted on ALN's site. If you are interested in the history of surgical instruments, take a look at Advancements in Surgery Through the Ages. But, there's more. We ran out of space to tell you about the barber surgeons. Read the exclusive here! Surgery in the Middle Ages It’s hard to imagine that surgery wasn’t always the prestigious profession it is today. But, in medieval Europe, physicians didn’t practice surgery, because such things were handled by lesser men… or even by women. Often, the local barber took care of minor surgeries. He would travel from town to town, and you could stop in to get a haircut and shave, and at the same time get a tooth pulled or have a minor surgery. In the 1500s, women were prohibited from bec
  4. Light Engine - Turning Light into Science
    April 20, 2015
    If you have seen a rainbow, you have seen the visible part of the light spectrum spread out. Each color we see is actually a different wavelength of light. In scientific applications, we are often interested in only one or two wavelengths of light. For example, a muscle tissue can be stained with Indo-1 or Fluo-4 causing intra-cellular calcium to fluoresce when excited by the appropriate wavelength of light. Or, a neuronal cell can be stained with di-4-ANEPPS and di-8-ANEPPS, causing a fluorescent signal that corresponds with the firing of an action potential. WPI’s new SI-BF-100 Biofluorometer is a light engine that generates quick pulses of light in one (or
  5. Setting Up Your Automated TEER Measurement Robot
    April 14, 2015
    Now that your REMS system has arrived, are you trying to figure out how to set it up? Check out this 5-minute video where we show you how, step-by-step. More Info
  6. Using WPI's PUL-1000 Micropipette Puller
    March 06, 2015
    World Precision Instruments' PUL-1000 is a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes used in intracellular recording, microperfusion and microinjection. It offers programmable sequences of up to four steps with complete control over the heating, force, movement and cooling time. This allows graduated cycles for a variety of applications. PUL-1000 can produce pipettes with tip diameters from less than 0.1µm to 10+ µm.  More Info
  7. FAQs about TEER Measurement
    February 02, 2015
    Here are some frequently asked questions (FAQ) about TEER measurement using an EVOM2. Are the electrical resistance and transepithelial electrical resistance (TEER) the same thing? What is an EndOhm chamber? How is an EVOM2 used for measuring confluence? Why use an EndOhm instead of a STX? How do I clean my electrode? What about electrode preconditioning? Can you give me a simple data acquisition system for TEER? What are the TEER measurement challenges that I may encounter? Can you suggest some experimental parameters that can be controlled to obtain consistent TEER results?
  8. Low Flow Dampening Kit for a Pump
    December 08, 2014
    Parts List Qty Part Number Description 2 13163 MALE LUER FITTING 5/32 2 13161 MALE LUER FITTING FOR 3/32 2 13157
  9. Recording TEER Measurements from an EVOM2
    November 11, 2014
    The EVOM2 is the classic, hand-held instrument for making Trans Epithelial Electrical Resistance (TEER) measurements. The REMS system adds a robot and data recording for automated TEER measurements. There may be times when you don't have a robot, but would still like to have the data recording capabilities of the REMS system. With a little ingenuity, you can do just that. Here, we will show you how you can add data recording to your standard EVOM2
  10. Using a DAM50 for EEG Recordings in Rodents
    October 21, 2014
    A low-noise amplifier like the DAM50 is an excellent choice for EEG recording in rodents. WPI’s amplifiers were engineered for the bio-medical researcher. While 20-30μV of noise is common in bio-amplifiers, WPI’s DAM series amplifiers generate 0.4μV RMS (root mean squared) at 0.1-100Hz. (That’s equal to about 2μV peak to peak.) This setup shows one way such recordings could be made. The RC1 electrode works well for rats, and the EP1 is more suitable for mouse cranial application. For this application, you will need the following equipment: (1) DAM50 amplifier (1) MD4R micromanipulator (2) M
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