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Blog
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January 03, 2019
The Butantan Institute, an agency linked to the State Department of Health of São Paulo and one of the largest biomedical research centers in the world, held the 4th edition of the Zebrafish Creation and Management university extension course in December where they exhibited WPI's Zebrafish Microinjection System.
The training is aimed at professionals and post-graduate students involved in zebrafish research as an animal model. The program addressed the physical characteristics, the environment of origin and the way of reproduction and growth of this fish. The coordinators are the researchers Mônica Lopes Ferreira and Carla Lima, responsible for the Zebrafish Platform of CeTICS Butantan whose current capacity holds 6,000 adult fish.
https://www.redezebrafish.com.br/parceiros
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November 28, 2018
Selection of Electrodes for TEER Measurements
Transepithelial electrical resistance (TEER), also referred as the transepithelial resistance (TER) is used to monitor cellular health. TEER is comprised of measurements of the transcellular pathway (i.e., resistance due to an individual cell) and paracellular pathway (i.e., resistance due to the formation of the cellular junctions). TEER is commonly used to monitor cellular confluence. TEER values can indicate changes in the cellular monolayer permeability, showing the monolayer barrier function of cells such as, endothelial (brain microvessel) and epithelial (alveolar, kidney, and intestinal) cells. High TEER values generally reflect tighter cellular monolayers or cellular junctions (Lewis 1996, Matter and Balda 2003, Denker and Sabath 2011). A few major benefits of WPI TEER measurement systems are described below. The TEER values (electrophysiological analysis) can be combined with other analysis methods to further understand...more
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October 08, 2018
Measuring CDOM with a Fiber Optic Liquid Waveguide Capillary Cell
One's imagination is the limit with practical uses of WPI's Liquid Wavelength Capillary Cells(LWCC), also referred to in the fiber optic spectroscopy community as a Long Pathlength Flow Cell. This fiber optic sampling accessory for absorbance measurements combines increased optical pathlengths with small sample volumes making them ideal for water analysis such as CDOM.
Two optical fibers couple to this sampling accessory one to send light to the sample cell and the second to send light to a spectrometer. The length of the fiber optic cable can vary allowing investigators to either spot test CDOM in the lab from collected water samples or continuously monitor CDOM of a body of water under investigation with this sampling accessory coupled to a fiber optic spectrometer and...more
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October 08, 2018
Jonas de Jesus, from WPI Brasil visit the winner of a contest to name a mascot for the Zebrafish Network. Jonas presented the winner with a surgical kit for zebrafish research.
The project "The Zebrafish Network" is a result of the partnership of the Center for Toxins, Immune Response and Cell Signaling (CeTICS) of the Butantan Institute, and aims to promote communication and integration, as well as foster collaborations between researchers studying the fish "Paulistinha ", Which has been used for many scientific studies because of its genetic similarity with humans (70%).
"The idea is that everyone who belongs to the network can divulge their work, interact with other researchers and inform about what is happening, such as publication of articles, lectures and congresses on the subject," explains Mônica Lopes , director of the Special Laboratory of Toxicology and coordinator of the Zebrafish Platform.
Since the Zebrafish Platform opened in the IB in 2015, a network...more
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August 22, 2018
Clean, Consistent Cut Every Time
When you need to quickly take minimally invasive, small samples, the biopsy punch is an easy choice. The biopsy punch is a hand held, pencil-shaped instrument with a slender, pencil-like body. It is lightweight with a hollow, circular, stainless steel, cutting tip.
In 1887 Edward Lawrence Keyes, the first president of the American Association of Genitourinary Surgeons, was the first doctor who documented the importance of using the biopsy punch for dermatological diagnostics. He observed that the skin tissue samples can be obtained without complications, minimal bleeding and no need for suturing.
Uses for Biopsy Punches
Punches are not solely used in dermatology. Biopsy punches are also designed for therapeutics, cosmetic...more
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July 16, 2018
This 900A video series of 6 videos is designed to help familiarize you with the basic operation of the 900A Micropressure System. The 900A was designed for measuring pressures in kidney tubules, and it has many applications. It has recently been used for measuring...more -
March 13, 2018
When you are selecting surgical instruments for a procedure, here are a few key points to consider - What procedure are you performing? Published research papers usually indicate which instruments other researchers have used for similar procedures. The correct surgical instrument for a particular procedure makes a difference on the outcome of that technique.
- What is the size of your subject? An instrument that is perfect for a 200–300 g rat (about 22–25 cm long) may not be the best choice for a neo-natal mouse of about 15 g (about 1–2.5 cm long).
- How often will the instrument be used? If you perform more than 100 cuts per day, a pair of titanium scissors or a pair of scissors with tungsten carbide inserts would be worth considering. They stay sharp longer.
In this article we will consider some of...more
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February 13, 2018
In this video you'll see how to replace the gasket in a Nanoliter2010.
If you have questions, contact us at 866.606.1974 (US Toll Free) or at [email protected]. To request a formal quote, please email us at [email protected].
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June 27, 2017
Absorption of light correlates to the energy of a photon that is taken-up by electrons of the substance atom. The electromagnetic energy is transformed into internal energy of the absorbent substance. The absorbance of a substance quantifies how much of the incident light is absorbed by it (instead of being reflected or refracted). Precise measurements of the absorbance at many wavelengths allow the identification of a substance via absorption spectroscopy, where a sample is illuminated from one side, and the intensity of the light that exits from the sample in every direction is measured (see Fig. 1). A few examples of absorption are ultraviolet–visible (UV-Vis) spectroscopy or infrared (IR) spectroscopy....more
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June 27, 2017
The use of fluorescent probes in cell physiology has emerged as indispensable tool in the analysis of cell functioning over recent years. The physics underlying fluorescence is illustrated by the electronic-state diagram (so-called Jablonski diagram, see Fig. 1), showing the three-stage process to create the fluorescent signal (Excitation - Excited/State Lifetime - Fluorescence Emission) in a fluorophore/indicator and simplified described below.
Fig. 1– Jablonski diagram illustrating the processes of fluorescence by absorption of higher photon energy by a fluorophore and subsequent emission of lower photon energy, resulting in fluorescence during the fluorescence-lifetime.
Fluorescence...more