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Blog
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April 22, 2021
The PUL-1000 is a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes. Here we will see how to load a piece of glass into the carriage.
The glass capillary is held by clamps mounted on two movable carriages. Both carriages synchronously slide as a program is executed.
- Manually slide the carriages together when you are loading the glass.
- To open the clamps, place your thumb under the clamp and depress the release button with your index finger.
- Slide the glass capillary into the groove from one side. Slide it through the center of heating filament onto the...more
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April 22, 2021
The PUL-1000 is a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes. Here are the resolutions for some common issues with the puller.
Puller Stops
If the puller stops in the middle of a pull and the unit beeps, one of two issues may be the culprit.
- The unit may be over heating. Allow the unit time to cool down before attempting to pull more glass.
- If a program fails in the middle of a pull, you may have exceeded the parameters of the unit. For example, when you add up the distance of travel for all the stages, you may have exceeded the maximum range of travel. Check your program. You may need to alter the heating or travel parameters to conform with the unit’s maximums.
Glass...more
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April 22, 2021
The PUL-1000 is a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes. We will show you how to run a glass softening test.
You should run the Softening Test when:
- You change the filament
- Lot numbers or capillary types change
- You create or modify a program
- The ambient environment changes
- Press the STOP key to quit any running program. The following window displays.
- Mount a glass capillary on the carriage.
- Press the START key to run the Glass Capillary Softening...more
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April 20, 2021
by Benjamin Dubansky, PhD, Animal Physiologist/Researcher
The EVOM is a special kind of voltmeter for measuring electrical properties across a layer of cells in culture or a biological membrane. Here's a few things I like about the new EVOM3.
There's a couple things I like about the EVOM3 better than the older models.
- You can charge it. These ones had batteries which are fine, and they had a power plug. But when you use the power plug, you get a little bit electrical noise, and the signal's not quite as stable. When you have a good battery in here, the charge lasts for quite some time. It charges pretty quickly. You...more
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April 20, 2021
by Benjamin Dubansky, PhD, Animal Physiologist/Researcher
An Ussing Chamber is used when performing barrier studies across an excised epithelial tissue. Similar measurements can be made in well plates using an EVOM series TEER Measurement Meter, which is used for epithelial tissue that is grown to confluence in a cell culture well. While EVOM measurements are only qualitative, your throughput is much greater with an EVOM3. The EVOMs are portable and much more economical than an Ussing Chamber. Here Ben Dubansky, PhD explains the differences between EVOMs and Ussing chambers.
The EVOM is a special kind of voltmeter for measuring electrical properties across a layer of cells in culture or a biological membrane. We are measuring TEER (TER) - Transepithelial Electrical Resistance. The EVOM3...more
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April 19, 2021
by Benjamin Dubansky, PhD, Animal Physiologist/Researcher
The EVOM is a special kind of voltmeter for measuring electrical properties across a layer of cells in culture or a biological membrane. It is the most commonly used commercial system for measuring across a layer of cultured cells. We are measuring TEER (TER) - Transepithelial Electrical Resistance. The EVOM3 allows us to zoom in and measure either resistance or voltage across these membranes or cells in culture.
Why was it invented?
The EVOM (Epithelial Volt Ohm Meter) was designed in the 80’s. It was invented back when multiwell culture plates were first introduced...more
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April 19, 2021
The Peri-Star Pro is a popular peristaltic pump. Here we will show you how to install the tubing in the pump head so that you can use the pump.
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First, remove the tubing fixture by pressing the Locking Tab on the left side of the fixture and pulling the fixture open.
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Then pull the tubing through the fixture. Make sure the tubing is straight inside the fixture with no kinks.
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Remount the fixture on the pump head. The rounded notch is on the right side of the fixture, and it fits against a steel rod on the Pump Head. When the other end of the fixture is pushed into place, the...more
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April 19, 2021
The Peri-Star Pro is a popular peristaltic pump. Here we will show how to set the system parameters for your application.
Using the Control Knob to Navigate the Menu
Press the Control Knob to enter the setup mode in the main menu, then rotate the Control Knob to toggle between the four functions. Press the Control Knob again when you get to System Setting. Turning the Control Knob toggles between the submenu items.
Define System Parameters
- Tubing ID is the first submenu. To change tubing inside diameter, click the Control Knob. After clicking, a list of tubing IDs appears. Turn to the desired tubing ID and click the Control Knob to select it.
- Test Time sets the flow time when performing a calibration. To change the time...more
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April 09, 2021
Let’s look at the PUL-1000, a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes. Here we will examine the design of the unit.
Tempered Glass Lid
For safety, the lid should be closed whenever you are pulling glass or moving the unit.
Capillary Glass Clamp Pads
These two silicone clamp pads securely hold the glass when you are pulling pipettes. Check the glass OD and install appropriate pads accordingly.
Heating Filament
The PUL-1000 comes with a circular platinum/iridium box filament, which may be easily replaced...more
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April 08, 2021
Vernier scales can be used on microscopes, stereotaxic frames and micromanipulators. The vernier scale was invented by French mathematician Pierre Vernier in 1631 as an upgrade on Pedro Nunes' measurement system for precision astrolobes. With a main scale and a sliding secondary scale, a vernier is used for making precise measurements.
How a Vernier Scale Works
The vernier scale is marked with divisions slightly smaller than the divisions of the main scale. For example, a vernier scale could have 11 markings for every 10 on the main scale. That's 10 divisions on the vernier scale for every 9 on the main scale. This means that the vernier divisions are each 90% of the main scale divisions. In this case, the 0-line and the 10-line...more