Recording TEER Measurements from an EVOM2

Recording TEER Measurements from an EVOM2The EVOM2 is the classic, hand-held instrument for making Trans Epithelial Electrical Resistance (TEER) measurements. The REMS system adds a robot and data recording for automated TEER measurements. There may be times when you don't have a robot, but would still like to have the data recording capabilities of the REMS system. With a little ingenuity, you can do just that. Here, we will show you how you can add data recording to your standard EVOM2.

  1. Open Labscribe3 on your computer. Let the program identify the data recorder. In our example, we will use a Lab-Trax-4.
    Recording TEER Measurements from an EVOM2
  2. Choose the default settings.
  3. Setup the preferences for one channel. If you like, you may add five points of smoothing.
    Recording TEER Measurements from an EVOM2 
  4. In the marks section of the main window, select A1 (channel one). This records a data point directly into the marks records window. Alternatively, the smoothed graph C1 may be selected.
  5. In the top graph, right click and choose Units>Simple to calibrate the graph to ohms.
  6. Enter these values for the EVOM2 conversion:
    • 2 point calibration
    • Apply units to new data
    • Value at Cursor 1: 0.0000 => 0.0000
    • Value at Cursor 2: 1.0000 => 1000
    • Unit Name = Ohms
  7. To connect the EVOM2 to the data recorder, connect a BNC cable from the Output BNC on the front of the EVOM2 to the Channel 1 input BNC on your data recorder.
  8. In the Marks window, enter A1 or Control. Move the electrode to the control well, and allow it to stabilize. Then, press Enter. Otherwise, it is difficult to hold the electrodes still while typing.

  9. For each additional well, enter in the number A2…, A3 into the Marks field. Then, move the electrode to that well and press the enter key after the reading stabilizes.

  10. Stop the recording and save the file.
  11. Open up the marks editor in Labscribe (View>Marks>Marks). The dialog box shows the time mark data and resistance values.
      
  12. Press the Export button and choose “All Marks.” Give your export file a name. The export files is a text file that may be import into Microsoft Excel or another spreadsheet application.

 

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