Why buy a PUL-1000

NanoFil™ is a unique low volume syringe developed for improved microinjection in mice and other small animals. RPE and IO kits are specially designed for “hands-free” and oil-free sub-microliter injection. Originally developed for retinal pigment epithelium and intraocular injection, they can also be used for other applications.

 

 

The NanoFil syringe system utilizes a seal system that is based on a 0.46 mm shank diameter. Any needle or tubing with an outside diameter of 0.46 mm should fit the NanoFil’s seal system. Here is how to install your needle in a way that prevents damage to the gasket and keeps your needles straight.

PRO TIP: Always use surgical gloves to prevent the oils from your skin from affecting the needle or the gasket.

  1. To change or install a needle, loosen the screw cap by turning it counterclockwise to release the compression of the seal. If there is a needle in place, it should pull out easily.

  2. Remove the screw cap and the gasket from the syringe.

  3. Install the replacement needle by pushing the shank of the needle into the open hole in the screw cap.

    TIP: Clean the thick part of the needle shank that goes into the gasket with alcohol to remove any oils left behind by handling and prevent the needle from slipping or moving. Otherwise, residual lubricants from your hands can allow the needle to slip or move from the zero set position.
  1. Place the gasket on the needle.

  2. Push the gasket up into the screw cap.
  3. Place the needle into the syringe body and align it to the zero mark. Push the needle in far enough to observe the shank through the glass syringe body. In general, the needle should be pushed in until the end of the shank is positioned at the zero mark on the scale. Going beyond this position will not damage the syringe or the needle, but it will prevent the plunger from being able to travel down to the zero mark.

  4. Once the shank is in the desired position, tighten the screw cap finger tight. Then test the security of the seal by pulling on the needle. It should not come out easily.

The NanoFil syringe plunger should only be used when the barrel is wet. Otherwise, friction can damage the Teflon® needle of the plunger. Do not pull the plunger repeatedly when the barrel is dry.

IF the needle is clogged, the plunger can generate a very high pressure exceeding 10,000 PSI due to the small cross sectional area of the piston. The gasket in the syringe will not be able to hold the needle in position under such a high pressure, so it may move or shoot out like a bullet. Therefore, never point the syringe needle at a person, and always use protective glasses when using the very small needle.

 

If you have any questions, give us a call at (866) 606-1974 (Toll free in the USA) or email us at [email protected].

 

Buy NANOFIL

 

NanoFil™ is a unique low volume syringe developed for improved microinjection in mice and other small animals. RPE and IO kits are specially designed for “hands-free” and oil-free sub-microliter injection. Originally developed for retinal pigment epithelium and intraocular injection, they can also be used for other applications.

 

RPE and IO kits are specially designed for “hands-free” and oil-free sub-microliter injection. Originally developed for retinal pigment epithelium and intraocular injection, they can also be used for other applications. The injection volume is controlled by UMP3’s program and is activated by a foot switch. This lets you use your “free hand” to place the needle into the injection site. These kits need to be used with a NanoFil syringe and UMP3 to achieve accurate, repetitive and oil-free injection in the sub-microliter range. The RPE kit has blunt needles, and the IO kit has the tri-surface beveled needles. 

SilFlex

SilFlex is flexible, low dead volume tubing made of fused silica with an OD of 460 µm, and It is 35 cm long. The dead volume of the tubing section is about 3 µL. The low dead volume, consistent inside diameter and flexibility are essential for the system. Handling this tubing gently. Sharp bending can crack the glass and damage the tubing permanently. Do not bend it with a radius less than 30 mm or against a sharp corner.

Needle Holder Nuts

The holder connects the SilFlex tubing with the injection needle. It has the same coupling mechanism used on the NanoFil syringe. The inside diameter of the barrel and gaskets are identical to the one on the syringe. Install both needle and tubing by loosening the screw, inserting the tubing and tightening the nut finger-tight. Insert the SilFlex into the red nut on the back of the holder. Insert your needle of choice through the front cap. Adjust the length of the needle by sliding the needle up into the holder. The needle and SilFlex should be very close to each other inside the holder barrel, but not touching. Because the tubing will move forward as the screw is tightened, a gap between the needle and the tubing is essential to avoid crushing the tubing. The gap can be as large as 1 mm while only increasing dead volume by 0.17μL. Because the SilFlex tubing is very soft, it might be difficult to “fish” it through the gasket during the installation. To solve this problem, unscrew the red nut and remove the silicone gasket. Thread the nut and the gasket onto the SilFlex tubing. Then, with the red nut and gasket on, fit the SilFlex into the back hole of the holder. Hold the SilFlex in the holder, slide the gasket and red nut into the holder and tighten it. 

Gasket

The gasket is yellow, and it has both a narrow and a wide opening to make the needle insertion easier.

 

If you have any questions, give us a call at (866) 606-1974 (Toll free in the USA) or email us at [email protected].

 

Buy NANOFIL