High Intensity LEDs Allow New Applications for the Biofluorometer

SI-BF-100The new SI-BF-100 is an LED-based fluorometer for life science applications. With up to seven LED modules (wavelengths), the SI-BF-100 covers many fluorometric applications in both Neuroscience and Cell Biology. This technology significantly cuts the cost of fluorescent imaging without sacrificing resolution or quality. WPI's recent introduction of the high intensity LED version of the Biofluorometer opens a host of new applications for life science researchers. 

In Vivo Applications

  • Monitor electrical activity over large areas of intact brain without the limitations of microelectrode arrays
  • Simultaneously monitor electrical activity and calcium concentration
  • Genetically-encoded fluorescent probes make it possible to monitor neurotransmitter behavior over entire regions of intact tissue
  • Integration with miniature fiber optic arrays or micro-scale endoscopes allow for measurements on animals while conscious and mobile

biofluorometer probeUsing the SI-BF-100 Biofluorometer equipped with high intensity LED modules and an appropriate fiber-optic probe, a researcher can perform many different types of analysis on intact tissue in vivo. Some potential applications include simultaneous measurement of membrane potential and calcium concentrations. With the use of genetically-encoded fluorescence probes, the applications are limited only by the imagination of the researcher.

The use of a probe allows for the measurement of entire areas of the intact brain without the limitations presented by the placement of arrays of microelectrodes. By removing these limitations, the researcher is able to collect data over larger groups of neurons without placing a single electrode.

Miniature fiber-optic arrays and microscale endoscopes have already been developed for use in Neuroscience. Coupling this technology with the SI-BF-100 allows the research to be conducted on conscious and mobile animals. By not requiring the animal to be attached to a traditional light source, more accurate results can be obtained.

The SI-BF-100 eliminates the need for expensive and complicated arrays of microelectrodes to conduct studies in vivo. Larger areas can now be completely analyzed at a fraction of the cost with a significant reduction in experimental complexity.

Microscopic Imaging Applications

  • Microscopic Imaging ApplicationsMonitor membrane potential over small networks in cell culture
  • Monitor surface membrane potential in a single cell
  • Simultaneously monitor electrical activity and calcium concentration
  • Integration of fluorescence microscopy onto existing patch-clamp setups
  • Genetically-encoded fluorescent probes make it possible to monitor neurotransmitter behavior between cells in cell culture samples
  • With the addition of a CCD, traditional spatial imaging can be conducted

Using the SI-BF-100 Biofluorometer equipped with high intensity LED modules and coupling to an inverted microscope, a researcher can perform many different types of analysis on both single cells and small cell cultures. Some potential applications include simultaneous measurement of membrane potential and calcium concentration. With the use of genetically-encoded fluorescence probes, the applications are endless.

The SI-BF-100 uses LED technology, eliminating the need for expensive secondary external light sources and project-specific filter cubes. Now, you can analyze several fluorophores simultaneously, without the need for expensive filter wheels and external timing devices. 

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